Trifluoroacetic Acid
Trifluoroacetic acid is a corrosive organofluorine compound that is structurally analogous to, but stronger than, acetic acid. Available in various quantities and reagent grades, it is used in NMR spectroscopy, mass spectrometry, organic synthesis, etc.
Almost 100,000-fold more acidic than acetic acid, TFA is widely used in organic chemistry. TFA is used as a reagent in organic synthesis because of its properties: volatility, organic solvent solubility, and acidic strength. It is less oxidizing than sulfuric acid and is more readily available in its anhydrous form than some other acids . Other features include:
- Used in acid-catalyzed reactions, especially peptide synthesis (to cleave esters)
- Dissolves protein when mixed with liquid SO2
- Removes t-butyl derived side-chain protecting groups in Fmoc peptide synthesis
- Can remove the t-butoxycarbonyl protecting group in organic synthesis
- At low concentrations, acts as an ion-pairing agent for peptides and small proteins in organic compound liquid chromatography
- For acid-stable materials, can be a solvent for NMR spectroscopy
- Acts as a calibrant in mass spectrometry
- Used to produce trifluoroacetate salts
- An ingredient in adhesives, sealants, paints, and coatings
When TFA combines with bases and metals, especially light metals, a strong exothermic reaction occurs. When mixed with lithium aluminum hydride (LAH), the reaction is explosive.
Although nonflammable, TFA is corrosive to skin, eyes, and mucous membranes and requires careful use and handling. It is harmful when inhaled, causes severe skin burns and eye damage, and is toxic to aquatic organisms at even low concentrations.
TFA is also a product of the metabolic breakdown of the anesthetic agent halothane. It is the suspected cause of halothane-induced hepatitis.
![Medchemexpress LLC (2S)-2-amino-3-[(3-fluorophenyl)-(2-phenylmethoxyphenyl)methoxy]propanoic acid; TFA | 00-00-0 | 99.8% | 509.45 g·mol⁻¹ | C25H23F4NO6 | 5 MG](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000263712.png-250.jpg)
Medchemexpress LLC (2S)-2-amino-3-[(3-fluorophenyl)-(2-phenylmethoxyphenyl)methoxy]propanoic acid; TFA | 00-00-0 | 99.8% | 509.45 g·mol⁻¹ | C25H23F4NO6 | 5 MG
ALX-1393 TFA is the trifluoroacetate salt of a selective glycine transporter 2 (GlyT2) inhibitor used as a research reagent to investigate glycinergic neurotransmission and nociception. It is supplied as a purified solid for laboratory use and has reported antinociceptive activity in rodent pain models.
- Selective GlyT2 inhibitor for probing glycinergic neurotransmission.
- Research-grade solid suitable for in vitro and in vivo studies.
- High purity (99.83%) supporting reproducible results.
- Molecular formula C25H23F4NO6 and molecular weight 509.45 g·mol⁻¹.
- Store at -20°C to maintain stability and minimize moisture exposure.
Medchemexpress LLC LLVK TFA | 585.66 | 25 MG
LLVK TFA is a selective IκB phosphorylation inhibitor. It reduces LPS-induced pro-inflammatory cytokines (IL-1β, IL-6, TNF-α). LLVK TFA is promising for research of inflammatory bowel disease and rheumatoid arthritis.
- Selective IκB phosphorylation inhibitor
- Reduces LPS-induced pro-inflammatory cytokines (IL-1β, IL-6, TNF-α)
- Promising for research of inflammatory bowel disease
- Promising for research of rheumatoid arthritis
![Medchemexpress LLC 2-Furancarboxamide, 5-nitro-N-[4-[7-(1,2,3,6-tetrahydro-4-pyridinyl)imidazo[1,2-a]pyridin-3-yl]phenyl] | 25 MG](https://assets.fishersci.com/TFS-Assets/CCG/product-images/default.jpg-250.jpg)
Medchemexpress LLC 2-Furancarboxamide, 5-nitro-N-[4-[7-(1,2,3,6-tetrahydro-4-pyridinyl)imidazo[1,2-a]pyridin-3-yl]phenyl] | 25 MG
W1131 TFA is a potent STAT3 inhibitor that induces ferroptosis. It inhibits cancer progression in subcutaneous xenograft, organoid, and PDX models of gastric cancer. It effectively alleviates cancer cell chemoresistance to 5-FU, and regulates the cell cycle, DNA damage response, oxidative phosphorylation, the IL6-JAK-STAT3 pathway, and the ferroptosis pathway.
- Potent STAT3 inhibitor
- Induces ferroptosis
- Inhibits cancer progression in subcutaneous xenograft, organoid, and PDX models of gastric cancer
- Alleviates cancer cell chemoresistance to 5-FU
- Regulates the cell cycle
- Regulates DNA damage response
- Regulates oxidative phosphorylation
- Regulates the IL6-JAK-STAT3 pathway
- Regulates the ferroptosis pathway
Medchemexpress LLC Balixafortide TFA (POL6326 TFA) | 1051366-32-5 | 99.9% | 1978.16 | 25 MG
Balixafortide TFA (POL6326 TFA) is a potent, selective, and well-tolerated peptidic CXCR4 antagonist with an IC50 less than 10 nM. It demonstrates significant selectivity for CXCR4, approximately 1000-fold, over various other receptors including CXCR7. This compound effectively blocks β-arrestin recruitment and calcium flux with IC50s less than 10 nM, functioning as a potent hematopoietic stem and progenitor cell (HSPC) mobilizing agent with observed anti-cancer effects.
- Potent and selective CXCR4 antagonist
- Demonstrates high selectivity over other receptors like CXCR7
- Blocks β-arrestin recruitment and calcium flux
- Functions as a potent hematopoietic stem and progenitor cell (HSPC) mobilizing agent
- Exhibits anti-cancer effects
- Optimized for favorable mouse ADME properties
Medchemexpress LLC Hexa-His TFA | 1456728-20-3 | 99.8% | 954.87 | 25 MG
Hexa-His TFA is a peptide consisting of 6 His residues, used as a metal binding site for the recombinant protein.
- Consists of 6 His residues
- Used as a metal binding site
- Suitable for recombinant protein applications
- Molecular weight of 954.87
- Purity of 99.8%
- Appearance is solid, white to off-white
- Recommended storage for powder: -80°C for 2 years or -20°C for 1 year
- Recommended storage in solvent: -80°C for 6 months or -20°C for 1 month
- Store sealed and away from moisture
Medchemexpress LLC Angiopeptin TFA | 2478421-60-0 | 99.01% | 1326.39 | 25 MG
Angiopeptin TFA is a cyclic octapeptide analogue of somatostatin. It acts as a weak sst2/sst5 receptor partial agonist with IC50 values of 0.26 nM and 6.92 nM, respectively. It is a potent inhibitor of growth hormone release and insulin-like growth factor-1 (IGF-1) production. Angiopeptin TFA also inhibits adenylate cyclase or stimulates extracellular acidification, making it valuable for research in coronary atherosclerosis.
- Acts as a partial agonist on the release of tritium on CHO hsst2 cells (in vitro).
- Significantly inhibits neointimal formation (in vivo).
- Significantly inhibits coronary artery myointimal proliferation in cardiac allografts (in vivo).
Medchemexpress LLC Apstatin trifluoroacetate | 99.4% | 573.56 g/mol | C25H34F3N5O7 | 5 MG
Apstatin TFA is the trifluoroacetate salt of apstatin, a potent aminopeptidase P (APP) inhibitor with reported Ki values of 2.6 μM (rat) and 0.64 μM (human). It has been used in preclinical studies showing cardioprotective effects and is supplied as a solid research compound for biochemical and pharmacological applications.
- Potent aminopeptidase P inhibitor with reported Ki values of 2.6 μM (rat) and 0.64 μM (human).
- Demonstrated cardioprotective effects in preclinical studies.
- Supplied as a solid with reported purity of 99.43%.
- Molecular formula C25H34F3N5O7 and molecular weight 573.56 g/mol.
- Recommended storage: powder -80°C (2 years) or -20°C (1 year); protect from moisture.
- Available in small research pack sizes suitable for preclinical experiments.
Medchemexpress LLC Ant308 (TFA) | 99.6% | 3467.10 (free base) | 1 MG
ANT308 TFA is a vasoactive intestinal polypeptide (VIP receptor) antagonist that significantly enhances the activation and proliferation of T cells. It inhibits the migration and metastasis and induces apoptosis of melanoma tumor cells by inhibiting VIP-VPAC2 signaling. This compound reduces the expression of MCAM and N-cadherin, making it suitable for studies on acute myeloid leukemia (AML) and uveal melanoma (UVM).
- Vasoactive intestinal polypeptide (VIP receptor) antagonist
- Enhances T cell activation and proliferation
- Inhibits migration and metastasis of melanoma cells
- Induces apoptosis in melanoma tumor cells
- Inhibits VIP-VPAC2 signaling
- Reduces MCAM and N-cadherin expression
- Useful for acute myeloid leukemia (AML) research
- Useful for uveal melanoma (UVM) research
Medchemexpress LLC Clovibactin TFA | 97.1% | 903.08 | 100 UG
Clovibactin TFA is the TFA salt form of Clovibactin. It is an antibiotic effective against drug-resistant bacterial pathogens without detectable resistance. Clovibactin TFA inhibits cell wall synthesis by targeting pyrophosphate of peptidoglycan precursors.
- Effective against drug-resistant bacterial pathogens
- Exhibits no detectable resistance
- Inhibits cell wall synthesis
- Targets pyrophosphate of peptidoglycan precursors
Medchemexpress LLC TfR-T12 TFA | 99.1% | 1604.75 | 1 MG
TfR-T12 TFA is a BBB-penetrated transferrin receptor (TfR) binding peptide. It binds to the TfR and is subsequently internalized into TfR-expressing cells, making it suitable for research applications.
- Displays a binding affinity in the nM range.
- Synthetic peptide obtained by phage display.
- Binds to a different site on TfRs compared with transferrin.
- Can be chemically conjugated to construct multifunctional lipid vesicles.
Medchemexpress LLC Mp6 (TFA) | 98.5% | 5 MG
mP6 (Myr-FEEERA-OH) is a myristoylated peptide and an integrin β3 inhibitor. It prevents the interaction of Gα13 with integrin β3 while preserving talin-dependent integrin function. This peptide also blocks the GTP usage of Rac1, Rap1, and Rab7, which effectively inhibits the infection of CHO-A24 cells.
- Myristoylated peptide
- Inhibits Gα13-integrin β3 interaction
- Preserves talin-dependent integrin function
- Blocks GTP usage of Rac1, Rap1, and Rab7
- Inhibits infection of CHO-A24 cells
- For research use only
Medchemexpress LLC Spinorphin (TFA) | 2828433-21-0 | 99.4% | 991.06 | 5 MG
Spinorphin TFA is an inhibitor of enkephalin-degrading enzymes. It inhibits aminopeptidase, dipeptidyl aminopeptidase III, angiotensin-converting enzyme, and enkephalinase. Spinorphin also has an antinociceptive effect.
- For research use only
Medchemexpress LLC Lys-CoA TFA (Lys-coenzyme A) | 98.5% | 25 MG
Lys-CoA TFA is a selective p300 histone acetyltransferase (HAT) inhibitor with an IC50 of 50-500 nM. It demonstrates over 100-fold selectivity for p300 compared to PCAF (IC50=200 μM). This compound inhibits p300 HAT activity-dependent transcriptional activation and is for research use only.
- Selective p300 histone acetyltransferase (HAT) inhibitor
- Displays greater than 100-fold selectivity for p300 over PCAF
- Inhibits p300 HAT activity-dependent transcriptional activation
- Induces a senescent phenotype in human normal melanocytes
Medchemexpress LLC Apraglutide TFA | 1295353-98-8 | 98.7% | 3765.25 | 5 MG
Apraglutide TFA is a synthetic 33-amino-acid peptide and a long-acting GLP-2 analogue. It enhances adaptation and linear intestinal growth in a neonatal piglet model of short bowel syndrome with total resection of the ileum.
- Long-acting GLP-2 analogue
- Enhances adaptation and linear intestinal growth
- Reduces fecal fat and energy losses
- Promotes intestinal lengthening, greater small-intestinal weight, longer villus height, and greater crypt depth