HPLC Columns
Waters Corp XTerra Prep RP18 Column, 19 x 300 mm, 10 μm
WATERS CORPORATION XTerra Prep RP18 Column, 19 x 300 mm, 10 μm
Waters Corp CORTECS C18+ Column, 90à , 1.6 µm, 3 mm X 150 mm, 3/pk
CORTECS C18+ Column, 90à , 1.6 µm, 3 mm X 150 mm, 3/pk
Waters Corporation C4 Column, 300 Å, 5 μm
Symmetry columns are designed to provide confidence in the long-term compliance of HPLC methods. They ensure consistent peak shape, selectivity, column-to-column and batch-to-batch reproducibility, and other critical performance characteristics. This column primarily supports legacy methods.
- Features C4 chemistry and reversed phase separation mode
- Silica particle substrate with a spherical shape
- pH range of 2 to 8
- Maximum pressure of 6000 psi (415 Bar)
- Endcapped with low silanol activity
- Molecular weight range of 1000 to 30000
- Pore size of 300 Å and particle size of 5 μm
- Dimensions: 2.1 mm inner diameter and 150 mm length
- 2.8 % carbon load
- Suitable for protein applications
- One unit per package
Waters Corp Waters Viridis Silica OBD Prep Column, 5 μm, 30 mm x 150 mm
Viridis Silica columns are engineered for high reproducibility and predictability, achieved through stringent product specifications and minimal metal content. They are suitable for both analytical screening and preparative purification, allowing optimization and scale-up of separation methods to preparative OBD columns.
- Spherical silica particles: 5 μm particle size, 0.9 cc/g pore volume, 100 Å pore size, and 340 m²/g surface area.
- High reproducibility and predictability from tight specifications and low metal content.
- Suitable for both analytical screening and preparative purification.
- Methods are scalable to preparative OBD columns.
- Good retention, peak shape, and selectivity in achiral SFC separations, with or without additives.
Waters Corp Spherisorb ODS1 Column, 80Å, 3 µm, 2 mm X 150 mm, 1/pk
Spherisorb ODS1 columns are general purpose, silica-based, reversed-phase C18 columns. The ODS1 packing features low ligand density with a specified carbon load of 6.2%.
Waters Corp Waters XTerra Phenyl Column, 125 Å, 3.5 μm, 3.9 mm × 150 mm
This XTerra Phenyl Column is a hybrid-based, reversed-phase column offering superior pH stability and unique selectivity for complex mixture separations, particularly for aromatic rings. It utilizes Hybrid Particle Technology for high separation efficiency and enhanced pH stability.
- Enhanced pH stability (1 to 12 pH range) due to its hybrid particle substrate.
- Provides unique selectivity for aromatic compounds and complex mixture separations.
- Ensures reproducible retention and excellent peak shape; features low silanol activity.
- Endcapped for improved performance, offering high efficiency and rugged mechanical strength.
- Compatible with mass spectrometry and designed for long lifetimes.
- Facilitates easy scale-up and allows for increased mass loading at high pH.
Waters Corp XTerra Shield RP18 Prep Column, 125Å, 5 µm, 7.8 mm X 150 mm, 1/pk
XTerra Preparative RP18 columns are hybrid-based, reversed-phase C18 columns that incorporate Waters patented embedded polar group technology to improve peak shape performance for highly basic compounds. These first generation columns provide superior pH stability compared to a silica-based column.
Waters Corp Waters BioSuite CM Cation-exchange Column, 1000Å, 10 μm, 7.5 mm x 75 mm
The BioSuite CM Cation-Exchange (CXC) Column features a strong sulfopropyl ligand coupled to a pH-stable (pH 2 to 12) methacrylic ester-based polymeric resin. These ion-exchange columns are designed for protein and peptide separations, offering flexibility for chromatographers to develop methods based on minor charge differences.
- Enables outstanding protein and peptide resolution and recovery.
- Offers scalability from analytical to lab-scale preparative applications.
- Incorporates high-performance chemistries for biomolecule isolation, analysis, and characterization.
- Provides flexibility for separating proteins/peptides based on charge variations.
- Available in nonporous and porous IEX options for varied resolution and binding capacity.
Waters Corporation BioSuite Phenyl Column, 10 μm, 1000 Å, 7.5 mm x 75 mm
Phenyl HIC columns provide an alternative to reversed-phase chromatography (RPC) for proteins and peptides sensitive to high organic solvents. Hydrophobic Interaction Chromatography (HIC) uses a weakly hydrophobic surface and salt gradient for precise, non-denaturing separation. The 10 μm column media features a phenyl group bonded to a methacrylic ester-based polymeric resin with a 1000 Å pore size, accommodating proteins up to 5,000,000 Daltons.
- Separates proteins and peptides based on hydrophobic characteristics.
- Offers a viable alternative to reversed-phase chromatography (RPC).
- Combines non-denaturing characteristics with HPLC precision.
- Phenyl group bonded to a methacrylic ester-based polymeric resin.
- Large 1000 Å pore size.
- Accommodates proteins up to 5,000,000 Daltons.
Waters Corp Waters SunFire Prep Column, C8, 100Å 10 μm, 30 × 250 mm
SunFire C8 columns are designed for general purpose method development at low pH. The C8 ligand offers less hydrophobicity and retentivity compared to C18, making it suitable for specific assays. These OBD (Optimum Bed Density) prep columns ensure robust performance.
- C8 chemistry for general purpose method development.
- Reversed phase with silica substrate.
- pH range: 2 to 8.
- Temperature limit: 45 °C.
- Max pressure: 6000 psi (415 Bar).
- Endcapped with low silanol activity.
- Spherical particle, 10 μm size.
- 100 Å pore size; 340 surface area.
- For HPLC preparative systems; LC/MS, LC techniques.
- L7 USP classification.
- Dimensions: 30 mm ID, 250 mm length.
- 12% carbon load.
- 1 unit per package.
Waters Corp WATERS CORPORATION Column, Shield RP8, 50 × 150 mm, 10 μm
The XTerra Shield RP8 Column is a preparative column designed for chromatography applications. It features specific dimensions and particle size for efficient separation.
- Preparative column type
- Dimensions of 50 × 150 mm
- Particle size of 10 μm
- Shield RP8 column type
Waters Corp Waters OBD Prep Column, BEH Shield RP18, 5 μm, 130 Å, 30 mm × 150 mm
XBridge BEH Shield RP18 columns are essential tools for HPLC method development, offering alternate selectivity particularly for phenolic compounds. An embedded carbamate group improves peak shape for basic compounds. Utilizing BEH particle technology, these columns are stable across a wide pH range and at high temperatures.
- Alternate selectivity, beneficial for phenolic compounds.
- Improved peak shape for basic compounds with embedded carbamate group.
- BEH technology for universal compatibility and excellent peak shape.
- Stable across a pH range of 2 to 11.
- Stable at temperatures up to 50°C (low pH) and 45°C (high pH).
- Maximum pressure of 6000 psi (415 Bar).
- Spherical particles, 5 μm size, 130 Å pore size.
- Compatible with LC/MS and LC techniques.
Waters Corp Waters Column, C8, 3.5 μm, 4.6 mm X 50 mm, 100 Å
This column is designed for general purpose method development at low pH. It offers high efficiency and enhanced sensitivity, utilizing a C8 ligand that is less hydrophobic and less retentive than C18, making it suitable for specific assays. Its excellent low pH stability ensures a longer column lifetime compared to other silica-based HPLC columns.
- Ideal for low pH method development.
- C8 ligand for less hydrophobic, less retentive separations.
- Extended column lifetime from low pH stability.
- High efficiency and sensitivity via patented silica synthesis.
- Supports mass spectrometry; delivers sharp peaks, low bleed.
- Achieves excellent resolution, reduced back pressure, and speed.
- Reliable and reproducible chromatographic results.
- Manufactured with high-purity materials under strict quality control.
GL Sciences Inc Reversed Phase Columns (C18 Column), 3 um, 4.6 mm, 150 mm
Generally, silica based columns are mechanically stable and provide high efficiencies, however, they cannot be used under alkaline conditions and their residual silanol groups tend to adsorb organic bases. InertSustain C18 employs a radically new type of silica, in which the surface of the silica is uniquely modified, enabling precise control of the silica properties.
