ELISA Substrates and Reagents
Meso Scale Discovery R-PLEX Human MPIF-1/CCL23 ASY
The R-PLEX Human MIF-1 (CCL23) Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures the chemokine Macrophage Migration Inhibitory Factor (MIF-1 or CCL23) in human serum plasma and cell culture supernatants This assay utilizes MSD s electrochemiluminescent detection technology and provides high sensitivity up to 5-logs of dynamic range and low sample volume requirements The kit includes a 96-well plate pre-coated with capture antibody detection antibody read buffer calibrator and sample diluent The assay can detect MIF-1 concentrations as low as 3 5 pg/mL with an upper limit of 100 000 pg/mL
ABclonal Technology Phospho-PPP1CA-T320 Rabbit pAb
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). This broadly expressed gene encodes the alpha subunit of the PP1 complex that associates with over 200 regulatory proteins to form holoenzymes which dephosphorylate their biological targets with high specificity. PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies suggest that PP1 is an important regulator of cardiac function and that PP1 deregulation is implicated in diabetes and multiple types of cancer. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Meso Scale Discovery R-PLEX Human NAP-2/CXCL7 ASYQP
The R-PLEX Human NAP-2 (CXCL7) Assay is a multiplex immunoassay kit from Meso Scale Diagnostics that allows for the quantitative measurement of NAP-2 (CXCL7) in human serum plasma and cell culture supernatants The assay utilizes MSD s electrochemiluminescent detection technology and is provided in a convenient 96-well plate format The assay has a detection range of 0 06-1500 pg/mL and requires 25 L sample size per well The total assay time is 3 5 hours This assay allows for the high-throughput sensitive and reproducible quantification of the neutrophil-activating chemokine NAP-2
ABclonal Technology p-RPS6-S235/236
Ribosomes the organelles that catalyze protein synthesis consist of a small 40S subunit and a large 60S subunit Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit The protein belongs to the S6E family of ribosomal proteins It is the major substrate of protein kinases in the ribosome with subsets of five C-terminal serine residues phosphorylated by different protein kinases Phosphorylation is induced by a wide range of stimuli including growth factors tumor-promoting agents and mitogens Dephosphorylation occurs at growth arrest The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA As is typical for genes encoding ribosomal proteins there are multiple processed pseudogenes of this gene dispersed through the genome
ABclonal Technology Phospho-NF-kB p65/RelA-S468 Rabbit pAb
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Immunology Consultants anti-Hamster (CHO) Legumain Antibody - Affinity Purified
This affinity‑purified antibody targets hamster (CHO) Legumain, a lysosomal cysteine protease involved in protein processing, antigen presentation, and cellular stress responses. Produced in chicken and purified to high specificity, it provides reliable detection of Legumain across CHO‑derived lysates, purified preparations, and process‑related impurity panels. Its robust performance makes it well suited for bioprocess monitoring, host cell protein characterization, and development of quantitative immunoassays. The antibody’s avian origin minimizes background in mammalian systems, supporting sensitive detection in ELISA, Western blotting, and other analytical workflows requiring consistent lot‑to‑lot performance.
Immunology Consultants anti-Hamster (CHO) Nidogen-1 Antibody - Affinity Purified
This affinity‑purified chicken antibody specifically recognizes hamster (CHO) Nidogen‑1, a key basement‑membrane glycoprotein involved in extracellular matrix organization, laminin binding, and structural stabilization of the pericellular environment. Its high specificity supports sensitive detection of Nidogen‑1 in CHO lysates, purified protein preparations, and host cell protein impurity panels. The avian host species minimizes background from mammalian samples, enabling clean performance in ELISA, Western blotting, and orthogonal analytical assays. Consistent purification ensures low nonspecific binding and reliable lot‑to‑lot behavior, making this reagent well suited for bioprocess monitoring, HCP characterization, and development of quantitative assays targeting CHO‑derived matrix proteins.