Real Time PCR Master Mixes
Takara Bio SYBR_ PREMIX EX TAQ_ (TLI RNAS
Reagent, PCR, Real-time; SYBR Premix Ex Taq DNA Polymerase (Tli RNase H Plus); Storage: 4 deg.C; 400 rxns
Alkali Scientific Radiant™ SYBR Green Fluorescein qPCR Kit, 10,000 Reactions, (100 x 1mL)
Radiant™ SYBR Green Flourescein qPCR Kit: Next-generation PCR buffer formulations for maximum PCR efficiency and reaction speed. Novel hot-start chemistry for improved specificity and sensitivity. Broad range detection for increased reliability in low-copy assays Versatile 2X mixes, suitable for both ultra-fast and standard cycling methods Compatible with BioRad iCycler™, MyIQ™ & IQ5™
New England Biolabs, Inc. Luna Universal qPCR Master Mix – 2500 rxn (1 x 25 ml)
The NEB Luna Universal qPCR Master Mix is an optimized 2X reaction mix for real-time qPCR detection and quantitation of target DNA sequences using the SYBR/FAM channel of most real-time qPCR instruments. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap with fluorescent dyes used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR, and existing as well as commercial qPCR assay primer sequences can be used.
Biotium Forget-Me-Not™ EvaGreen qPCR Master Mix (High ROX) (500 reactions)
Forget-Me-Not™ EvaGreen qPCR Master Mix is a high performance, hot-start EvaGreen dye-based master mix for use in real time PCR applications and DNA melt curve analysis. Forget-Me-Not™ master mix has a light blue master mix color which helps prevent pipetting mistakes, saving time and reagents. The master mix features EvaGreen Dye which is suitable for HRM analysis and is safer, more environmentally friendly, and more sensitive than SYBR Green. The kit also features Cheetah™ Hotstart Taq for fast cycling protocols and High ROX reference dye. This product has sufficient reagent for 500 PCR reactions.
Biotium Forget-Me-Not™ EvaGreen qPCR Master Mix (High ROX) (2000 reactions)
Forget-Me-Not™ EvaGreen qPCR Master Mix is a high performance, hot-start EvaGreen dye-based master mix for use in real time PCR applications and DNA melt curve analysis. Forget-Me-Not™ master mix has a light blue master mix color which helps prevent pipetting mistakes, saving time and reagents. The master mix features EvaGreen Dye which is suitable for HRM analysis and is safer, more environmentally friendly, and more sensitive than SYBR Green. The kit also features Cheetah™ Hotstart Taq for fast cycling protocols and High ROX reference dye. This product has sufficient reagent for 2000 PCR reactions.
Alkali Scientific Radiant™ SYBR Green Fluorescein qPCR Kit, 2,000 Reactions, (20 x 1mL)
Radiant™ SYBR Green Flourescein qPCR Kit: Next-generation PCR buffer formulations for maximum PCR efficiency and reaction speed. Novel hot-start chemistry for improved specificity and sensitivity. Broad range detection for increased reliability in low-copy assays Versatile 2X mixes, suitable for both ultra-fast and standard cycling methods Compatible with BioRad iCycler™, MyIQ™ & IQ5™
Medchemexpress LLC HY-K0501 1 mL (100 rxns) Medchemexpress, SYBR Green qPCR Master Mix CAS: Purity:>98%
Medchemexpress, HY-K0501 1 mL (100 rxns) SYBR Green qPCR Master Mix CAS: MCE SYBR Green qPCR Master Mix is provided as a simple-to-use, stabilized 2X formulation that includes all components for qPCR except sample DNA, primers and water. Purity:>98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
Biochain Institute Inc Power Eva QPCR SuperMix Kit, 200 rxn/PK
BioChain's QPCR SuperMix is a 2x concentration of premix reagent including Hotstart DNA polymerase and specially formulated real time buffer designed for real-time PCR with intercalator format. Eva and SYBR Green QPCR Supermix is in double-stranded cDNA detection format, while Pro QPCR Supermix is in the format of fluorescent probe-based detection assays, such as TaqMan or molecular beacons.
Sigma Aldrich Fine Chemicals Biosciences fastStart TaqManR Probe Ma
Hot start protocols have been shown to significantly improve the specificity sensitivity and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of fastStart Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore there is no elongation during the period when primers can nonspecifically bind. The fastStart Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e. a pre-incubation step at 95C).
New England Biolabs, Inc. NEBNext Q5 Hot Start HiFi PCR Master Mix – 50 reactions
NEBNext Q5 Hot Start HiFi PCR Master Mix is the original hot start NEBNext formulation of Q5 High-Fidelity DNA polymerase. This formulation is also included as a component in all of the original NEBNext Ultra kits, for DNA and RNA library preparation. However, the NEBNext Ultra II Q5 Master Mix is now available, and this more recent hot start formulation further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions.