Real Time PCR Master Mixes
New England Biolabs, Inc. WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) – 500 reactions (25 µl vol)
The WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow. This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15-40 minutes.
Bio Rad Laboratories SYBR Green Supermix, 100 reactions, 2.5 ml
SYBR Green Supermix, 100 reactions, 2.5 ml
Bioneer AccuPower PCR PreMix Tube, 20 μl reaction volume, 0.2 ml thin-wall 8-strip tubes, 480 tubes, Lyophilized premix pellet
AccuPower PCR PreMix Tube, 20 μl reaction volume, 0.2 ml thin-wall 8-strip tubes, 480 tubes, Lyophilized premix pellet
VWR INTERNATIONAL MultiPlex qPCR SuperMix, 200 x 50 uL
PerfeCTa Multiplex qPCR SuperMix, Low ROX is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers, probe(s), and template for real-time quantitative PCR on Applied Biosystems 7500 or Stratagene MX series of real-time PCR systems.
Alkali Scientific Radiant™ SYBR Green Fluorescein qPCR Kit, 2,000 Reactions, (20 x 1mL)
Radiant™ SYBR Green Flourescein qPCR Kit: Next-generation PCR buffer formulations for maximum PCR efficiency and reaction speed. Novel hot-start chemistry for improved specificity and sensitivity. Broad range detection for increased reliability in low-copy assays Versatile 2X mixes, suitable for both ultra-fast and standard cycling methods Compatible with BioRad iCycler™, MyIQ™ & IQ5™
New England Biolabs, Inc. WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG)- 100 rxn
The WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) is designed to provide a simple one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provide rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This mix contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized buffer solution. The inclusion of thermolabile UDG prevents carryover contamination where unintended product of a previous amplification can serve as the substrate of a subsequent reaction. The WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) is compatible with multiple detection methods including turbidity detection real-time fluorescence detection and end-point visualization.
New England Biolabs, Inc. WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG)- 500 rxn
The WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) is designed to provide a simple one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provide rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This mix contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized buffer solution. The inclusion of thermolabile UDG prevents carryover contamination where unintended product of a previous amplification can serve as the substrate of a subsequent reaction. The WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) is compatible with multiple detection methods including turbidity detection real-time fluorescence detection and end-point visualization.
New England Biolabs, Inc. LunaScript RT Master Mix Kit (Primer-free) - 25 rxn
LunaScript RT Master Mix Kit (Primer-free) is an optimized master mix containing all the necessary components for first strand cDNA synthesis except for primers. The mix is compatible with random primers, oligo dT primers, and gene-specific primers enabling maximum cDNA synthesis flexibility. LunaScript RT Master Mix (Primer-free) features the thermostable Luna Reverse Transcriptase which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is included to protect template RNA from degradation. In addition, the presence of a blue tracking dye provides a visual indicator for the RT step as well as downstream applications.
New England Biolabs, Inc. LunaScript RT SuperMix - 500 rxns
LunaScript RT SuperMix is an optimized master mix containing all the necessary components for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It features the thermostable Luna Reverse Transcriptase which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is included to protect template RNA from degradation. LunaScript RT SuperMix contains random hexamer and poly-dT primers allowing even coverage across the length of the RNA targets. In addition LunaScript RT SuperMix contains dNTPs and a blue tracking dye providing a visual indicator that can be followed throughout the two-step RT-qPCR process. This product offers robust linear and sensitive detection using total RNA inputs as high as 1 ug and as low as single copies of RNA.
Roche Diagnostics KAPA SYBR FAST qPCR Kits - 1000 x 20 uL reactions (Universal), 10 mL
KAPA SYBR FAST qPCR Kits - 1000 x 20 uL reactions (Universal), 10 mL. *This item is non returnable.