Real Time PCR Master Mixes
Sigma Aldrich Fine Chemicals Biosciences fastStart TaqManR Probe Ma
Hot start protocols have been shown to significantly improve the specificity sensitivity and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of fastStart Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore there is no elongation during the period when primers can nonspecifically bind. The fastStart Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e. a pre-incubation step at 95C).
New England Biolabs, Inc. LunaScript RT Master Mix Kit (Primer-free) - 25 rxn
LunaScript RT Master Mix Kit (Primer-free) is an optimized master mix containing all the necessary components for first strand cDNA synthesis except for primers. The mix is compatible with random primers, oligo dT primers, and gene-specific primers enabling maximum cDNA synthesis flexibility. LunaScript RT Master Mix (Primer-free) features the thermostable Luna Reverse Transcriptase which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is included to protect template RNA from degradation. In addition, the presence of a blue tracking dye provides a visual indicator for the RT step as well as downstream applications.
Sigma Aldrich Fine Chemicals Biosciences fastStart SYBR Green Maste
fastStart SYBR Green Master Instructions for UsefastStart(TM) SYBR(R) Green Master is a ready-to-use hot start reaction mix without ROX for quantitative polymerase chain reaction (qPCR) and reverse transcription (RT)-qPCR on real-time PCR systems other than the LightCycler(R) instruments. This master mix simplifies the preparation of reactions for DNA detection and analysis. In combination with a real-time PCR instrument suitable PCR primers and a hydrolysis probe fastStart(TM) TaqMan(R) Probe Master allows very sensitive detection and quantification of defined DNA sequences.SYBR(R) Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis the SYBR(R) Green I dye included in the reaction mix binds to the amplified PCR products. The amplicon can be detected by its fluorescence.Hot start protocols have been shown to significantly improve the specificity sensitivity and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of fastStart(
Sigma Aldrich Fine Chemicals Biosciences KOD OneTM PCR Master Mix -
KOD One(TM) PCR master Mix -Blue- is a ready-to-use 2 x PCR master mix containing a novel genetically modified KOD DNA polymerase (UKOD) along with a new elongation accelerator enabling fast PCR with an extension time of 5 sec/ kb for template DNA 10kb. This master mix has greater efficiency and flexibility than conventional PCR enzymes with an ability to amplify from crude specimens such as blood and mouse cell lysates. In addition to crude samples it can be used on templates containing uracils (dU). The master mix can use primers containing both inosines (dI) and dU for amplification.This hot start KOD polymerase is antibody-inactivated with two types of antibodies that prevent the 5-3 polymerase activity and 3-5 exonuclease activity. Upon heat-activation the 3-5 proof-reading ability of the enzyme generates blunt-end PCR products.
GenDEPOT amfiSure ONE PCR Master Mix(2X), P7000, 5 x 1 mL
amfiSure ONE PCR Master Mix is ready-to-use mixtures that include all of the reagents needed for PCR. amfiSure PCR Master Mix provides superior yields in both routine and challenging PCR application such as direct PCR using crude extracted DNA, GC rich region PCR, colony PCR, multiplex PCR and etc.
New England Biolabs, Inc. Luna Universal qPCR Master Mix – 1000 rxn (10 x 1 ml)
The NEB Luna Universal qPCR Master Mix is an optimized 2X reaction mix for real-time qPCR detection and quantitation of target DNA sequences using the SYBR/FAM channel of most real-time qPCR instruments. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap with fluorescent dyes used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR, and existing as well as commercial qPCR assay primer sequences can be used.
New England Biolabs, Inc. Luna Universal Probe qPCR Master Mix – 200 rxn (2 x 1 ml)
The NEB Luna Universal Probe qPCR Master Mix is a 2X reaction mix optimized for real-time qPCR detection and quantitation of target DNA sequences using hydrolysis probes. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap fluorophores commonly used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers/probes and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR and existing as well as commercial qPCR assay primer/probe sequences can be used.
New England Biolabs, Inc. Luna Universal qPCR Master Mix – 2500 rxn (1 x 25 ml)
The NEB Luna Universal qPCR Master Mix is an optimized 2X reaction mix for real-time qPCR detection and quantitation of target DNA sequences using the SYBR/FAM channel of most real-time qPCR instruments. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap with fluorescent dyes used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR, and existing as well as commercial qPCR assay primer sequences can be used.
Bio Rad Laboratories SsoAdvanced Universal SYBR Green Supermix, 2 ml (2 x 1 ml vials), 200 x 20 μl reactions, 2x qPCR mix, Sso7d fusion protein technology
SsoAdvanced Universal SYBR Green Supermix, 2 ml (2 x 1 ml vials), 200 x 20 μl reactions, 2x qPCR mix, Sso7d fusion protein technology