Cellular Imaging Instrumentation Accessories
Sigma Aldrich Fine Chemicals Biosciences 3dGROTM Lung Organoid Bran
The lung epithelium undergoes a complex series of endodermndashmesoderm-mediated signaling events to generate a complex arborized network of conducting airway (bronchi bronchioles) and gas-exchanging units (alveoli). Lung epithelium differentiation stages include anterior/posterior and dorsal/ventral patterning lung specification lung budding branching morphogenesis and maturation. The 3dGRO(TM) Lung Organoid Branching Medium is a chemically defined/serum-free ready-to-use media used to differentiate Sox2/Pax6 anterior foregut endoderm (AfE) cells into lung bud organoids in suspension cultures. Additionally the 3dGRO(TM) Lung Organoid Branching Medium can be used to culture AfE cells on fibronectin coated cultureware. The 3dGRO(TM) Lung Organoid Branching Medium is a component of the 3dGRO(TM) Human Lung Organoid Culture System which can be used to differentiate human iPS cells into mature lung organoids that structurally resemble the in vivo branching airway and early alveolar s
Sartorius Incucyte Labeling Kit
Extracellular vesicles EVs are heterogeneous lipid-bound particles 50 – 1000 nm naturally secreted by cells into the extracellular space. Exosomes a subclass of EVs are defined by their size 40 to 160 nm and biogenesis endosomal origin . EVs which transport biomolecules like proteins lipids and nucleic acids are of interest to researchers for diagnostic and therapeutic purposes. The Incucyte Exofluor Green EV Labeling Kit allows for real-time evaluation of cellular EV uptake and is validated for use with the Incucyte Live-Cell Analysis System.
Alkali Scientific CellPro™ Spheroid Dish, PS / PE, 35mm x 10mm, Sterile 15/Cs, .20mL Low Binding Treatment
The CellPro™ Spheroid Dish is a specialized dish designed for spheroid formation in cell culture experiments. With a low binding treatment and a 0.20mL capacity, this dish ensures optimal performance in laboratory settings. It is 35mm x 10mm in size and comes in sterile packaging with 15 dishes per case. Ideal for research on cellular interactions and behavior, this product provides reliable and high-quality results for spheroid-related studies.
Sartorius Incucyte S-Series Incustore
Ideal if you use your Incucyte intensively as it keeps data on-line and at your fingertips for longer. The RAID 6 configuration of 8 x 6TB hard disks protects all data. If a drive fails you can rest assured that there's no downtime. Simply swap out the drive from the front of the unit and allow the RAID to rebuild.
Alkali Scientific CellPro™ Spheroid Forming Unit, PC / HDPE / PTFE / PP / PET, 17mm x 120mm, 15mL, Sterile, 90/Cs
The CellPro™ Spheroid Forming Unit, designed for cell culture applications, is a sterile unit made from high-quality materials such as PC, HDPE, PTFE, PP, and PET. With a 15mL capacity and 17mm x 120mm dimensions, it is perfect for spheroid formation in laboratory experiments. This unit ensures high-quality results in cell-based research, providing a reliable platform for researchers to study cell behavior and interactions.
FUJIFILM Biosciences sOFTWARE
Software that supports data processing based on the protocols complying with three types of pharmacopeia USP EP JP for bacterial endotoxin testing compliant with FDA 21 CFR Part 11 ERES electronic records/electronic signature and offers excellent audit trail capabilities can provide statistical processing of means standard deviations and has robust data processing functions The data processing functions can be performed by defining sample types such as standards controls and test samples
Sartorius Incucyte Imagelock plate
The Incucyte Imagelock 96-well Plate is a specially designed 96-well microtiter plate that contains fiducial marks that allow for precise image registration of the same field of view even when the plates are manually removed and replaced within the Incucyte Live-Cell Analysis System. However these markings are subtle enough to not interfere with images acquired at the cell plane. This enables accurate image analysis that is required for scratch wound cell migration and invasion assays when used with the Incucyte Live-Cell Analysis System and software.
Sartorius Incucyte 3D Nanowell Plate
The Incucyte 3D Nanowell Plates facilitate high-throughput organoid studies by creating a 3D microenvironment that mimics in vivo conditions supporting organoid growth and differentiation. These plates feature an array of hydrogel-printed microwells each optimized to foster uniform and reproducible organoid formation. Additionally each well contains a dedicated pipetting port for seamless medium changes that can be integrated with automated processes thus enhancing efficiency and reproducibility in large-scale screening assays. Together with the Incucyte Live-Cell Analysis System and Incucyte Organoid Analysis Software Module 9600-0034 these plates enable high-throughput long-term imaging and analysis of organoids providing insights into their growth and viability dynamics. This plate also requires systems to be operating on Incucyte Base Software v2024A or later.
Sartorius Incucyte Neuroactive Kit
The Incucyte Neuroactive Kit contain cryopreserved vials of rat cortical neurons and astrocytes isolated from embryonic stage E18 Sprague-Dawley rats as well as sufficient Incucyte Neuroburst Orange Lentivirus to perform one 96-well experiment.The Incucyte Neuroburst Orange Lentivirus is a lentiviral based live-cell neuronal labeling reagent driven by a synapsin promoter resulting in the long term expression of a genetically encoded orange fluorescent calcium indicator mRuby-based in neuronal cells.
INTACT GENOMICS INC ig Recombinase Polymerase Amplification (RPA) Kit v2, 25 Reactions
The improved IG Recombinase Polymerase Amplification Version 2 Kit (IG RPA Kit v2) amplifies DNA at a single and constant temperature (25°C – 42°C) using a recombinase (e.g. UvsX), primers, a single-stranded DNA binding protein (SSB), and a strand displacing DNA polymerase. T4 UvsX is used in combination with its accessory protein, UvsY. The recombinase interacts with the primers to form nucleoprotein filaments. This complex can bind with homologous double-stranded DNA through a strand exchange. After the exchange, a single-stranded binding protein, T4 gp32, stabilizes the displaced strand. Finally, Bsu/Sau DNA polymerase extends the DNA from the primers, creating a new complete copy of the template, and amplification can continue as in Polymerase Chain Reaction (PCR). IG RPA v2 offers More robust amplification and more specific amplification of the primary target than Version 1 for some applications. It may reduce background amplification of off-target sites.
Sartorius Incucyte rAstrocytes
Incucyte rAstrocytes are isolated from the cortex of Sprague Dawley Rats at day 18 of gestation and cryopreserved at 2 000 000 per vial. The ready-to-use vials are intended for use within the Incucyte Live-Cell Analysis System in 96-well co-culture assays with Incucyte rCortical Neurons Cat. No. 4753 for live-cell analysis of Incucyte Neuronal Activity or Incucyte Neurite Analysis.
INTACT GENOMICS INC ig Recombinase Polymerase Amplification (RPA) Kit v2, 100 Reactions
The improved IG Recombinase Polymerase Amplification Version 2 Kit (IG RPA Kit v2) amplifies DNA at a single and constant temperature (25°C – 42°C) using a recombinase (e.g. UvsX), primers, a single-stranded DNA binding protein (SSB), and a strand displacing DNA polymerase. T4 UvsX is used in combination with its accessory protein, UvsY. The recombinase interacts with the primers to form nucleoprotein filaments. This complex can bind with homologous double-stranded DNA through a strand exchange. After the exchange, a single-stranded binding protein, T4 gp32, stabilizes the displaced strand. Finally, Bsu/Sau DNA polymerase extends the DNA from the primers, creating a new complete copy of the template, and amplification can continue as in Polymerase Chain Reaction (PCR). IG RPA v2 offers More robust amplification and more specific amplification of the primary target than Version 1 for some applications. It may reduce background amplification of off-target sites.