Enzymes and Inhibitors
New England Biolabs, Inc. I-SceI – 500 units
A restriction endonuclease that recognizes the sequence TAGGG_ATAA^CAGGGTAAT. I-SceI is an intron-encoded endonuclease. The intron encoding I-SceI is present in the mitochondria of Saccharomyces cerevisiae.
New England Biolabs, Inc. XbaI – 15000 units
A restriction endonuclease that recognizes the sequence T^CTAG_A.
New England Biolabs, Inc. PmlI – 10000 units
A restriction endonuclease that recognizes the sequence CAC_^GTG.
Apexbio Technology LLC Dpn I(Synonyms: DpnI, DpnI Restriction Enzyme, Dam methylation-sensitive restriction enzyme DpnI), 500U.
Dpn I is a restriction endonuclease sensitive to Dam methylation specifically recognizing and cleaving the adenine N6-methylated sequence GA/TC within DNA Cleavage is dependent on adenine methylation status mammalian CpG methylation overlapping Dpn I sites blocks cleavage and methylation by Dcm is not recognized by this enzyme It is routinely used for the rapid digestion of plasmid DNA PCR fragments and genomic DNA and finds broad utility in genetic typing molecular cloning Southern blotting SNP genotyping and restriction fragment length polymorphism (RFLP) analyses
New England Biolabs, Inc. SacI-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence G_AGCT^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
ABclonal Technology BstEII
5...G/ G T N A C C...3 3...C C A N T G/ G...5 Isoschizomers: Eco91I BstPI EcoO65I PspEI
Cayman Chemical BAM-22Phumnmouseratb 5mg
An endogenous neuropeptide derived from proenkephalin A; expressed in the adrenal medulla and at lower concentrations throughout the brain; binds the µ-, κ-, and δ-opioid receptors (Kis = 0.9, 2.9, and 7.2 nM, respectively); intracerebroventricular administration induces analgesia in a tail-flick assay in mice (ED50 = 6.4 nM); decreases the frequency of tail flicking, as well as paw lifting and licking in a rat model of formalin-induced nocifensive behavior at 5 nM/animal
New England Biolabs, Inc. HpaI – 500 units
A restriction endonuclease that recognizes the sequence GTT_^AAC.
New England Biolabs, Inc. MscI – 1250 units
A restriction endonuclease that recognizes the sequence TGG_^CCA.
New England Biolabs, Inc. BglII – 2000 units
A restriction endonuclease that recognizes the sequence A^GATC_T.
New England Biolabs, Inc. BglII – 10000 units
A restriction endonuclease that recognizes the sequence A^GATC_T.
New England Biolabs, Inc. EcoRI – 10000 units
A restriction endonuclease that recognizes the sequence G^AATT_C.