Enzymes and Inhibitors
Biotium Coelenterazine (ALSO KNOWN AS NATIVE COE
Coelenterazines are luminescent enzyme substrates for apoaequorin and Renilla luciferase. Apoaequorin is used as a calcium indicator whereas Renilla luciferase is commonly used as a reporter of transcription regulation. Coelenterazine binds apoaequorin to form an aequorin complex which emits light upon calcium binding, and can measure a broad range of calcium concentrations from ~0.1 uM to >100 uM. Renilla luciferase is widely used as a reporter protein and as a donor in bioluminescence resonance energy transfer (BRET) to study protein-protein interactions. Coelenterazines can also be used for chemiluminescent detection of superoxide anion and peroxynitrite in cells or tissues.Coelenterazine (native): Yellow solid soluble in MeOH or EtOH; Store at -20°C and protect from light; C26H21N3O3; MW: 423; [55779-48-1]. See also other Coelentrazine analogs and water-soluble Aquaphile™ Coelenterazines (native and analog h) for in vivo use.
Abcam D-(+)-Cellobiose, beta-glucosidase substrate, 5G
MW 342.3 g/mol, Purity >99%. Disaccharide consisting of two β-glucose molecules linked by a β-1,4 glycosidic bond. Used as a substrate of β-glucosidase.
The product is subject to the following: Abcam Restricted Use Statement
Immunology Consultants Lysosomal Acid Lipase (Host Cell Protein) ELISA Kit – CHO (Chinese Hamster Ovary)
The Lysosomal Acid Lipase (Host Cell Protein) ELISA Kit – CHO (Chinese Hamster Ovary) is a highly sensitive immunoassay designed to detect Lysosomal Acid Lipase (LAL), a lipolytic enzyme involved in the breakdown of cholesteryl esters and triglycerides within lysosomes. As a residual host cell protein released from CHO cells during biologics manufacturing, LAL may contribute to polysorbate degradation and product stability risks if not effectively removed during purification.This assay enables precise quantification of residual LAL in bioprocess samples and supports manufacturers seeking greater visibility into high-risk lipase contaminants. This kit is ideal for HCP monitoring, bioprocess development, and biopharmaceutical quality control, helping manufacturers better detect problematic lipases that may impact formulation stability and product quality.
Research Products International Corp X-GAL and IPTG Ready to Use Non-Toxic Solution 10mg/ml, 500 Milligrams
Stable solution of X-GAL and IPTG. Does not contain DMSO or DMF. Solution concentration is 10 mg/ml X-Gal and 8 mg/ml IPTG.
Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. WARNING! This product can expose you to 1-Methyl-2-Pyrrolidon, a chemical which is known to the State of California to cause developmental harm. For more information go to www.P65Warnings.ca.gov.
New England Biolabs, Inc. Antarctic Thermolabile UDG – 100 units
Antarctic Thermolabile UDG (Uracil- DNA Glycosylase) catalyzes the release of free uracil from uracil-containing single-stranded or double-stranded DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperature and high pH. This enzyme is sensitive to heat and can be rapidly and completely inactivated at temperatures above 50C.
New England Biolabs, Inc. MspJI – 1000 units
A restriction endonuclease that recognizes the sequence CNNRNNNNNNNNN^NNNN_.
Gold Biotechnology Inc D Luciferin Potassium Salt 3g
L uciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2 as cofactors to emit a characteristic yellow-green emission in the presence of oxygen which shifts to red light in vivo at 37 C Through the utilization of ATP the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain
New England Biolabs, Inc. BmtI-HF – 1500 units
A high fidelity restriction endonuclease that recognizes the sequence G_CTAG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
Sigma Aldrich Fine Chemicals Biosciences Lipase from human pancreas
Pancreatic lipase fulfills a key function in dietary fat absorption by hydrolyzing triglycerides into diglycerides and subsequently into monoglycerides and free fatty acids.
Sigma Aldrich Fine Chemicals Biosciences Ribonuclease H RNase H fro
Ribonuclease H (RNase H) is a nonspecific endoribonuclease localized to the nucleus and cytoplasm. It is ubiquitously found and widely present among many organisms including viruses and human.
Sigma Aldrich Fine Chemicals Biosciences D Luciferin sodium salt ly50MG
D-Luciferin is mainly produced in bioluminescent organisms. It can simply circulate across the blood testis barrier blood brain barrier and the blood placenta barrier. This small molecule is sensitive to light and oxygen. D-Luciferin is also sensitive to moisture in its powder form.