Enzymes and Inhibitors
Neogen Diagnostics K-Gold PNPP Substrate, 200 mL, Microwell, AP, Colorimetric
K-Gold Substrate is a one-bottle stabilized chromogenic substrate for use with alkaline phosphatase based immunoassays. K-Gold Substrate utilizes the substrate p-nitrophenyl-phosphate (PNPP), which reacts with alkaline phosphatase to produce the chromophore p-nitrophenol. NEOGENs ready-to-use K-Gold formula does not require mixing of additional ingredients or stabilizing agents, making it an ideal formula for kit manufacturers. Stable for a minimum of 30-months when stored at 2-8C.
Medchemexpress LLC Boc-Lys(Ac)-AMC | 233691-67-3 | MFCD02683946 | 99.5% | C23H31N3O6 | 10MG
Boc-Lys(Ac)-AMC is a cell-permeable fluorogenic substrate for histone deacetylases (HDACs). Upon enzymatic deacetylation and subsequent proteolytic cleavage, it releases 7-amino-4-methylcoumarin (AMC), producing a fluorescent signal suitable for quantitative fluorometric assays and inhibitor screening (Ex/Em = 355 nm/460 nm).
- Cell-permeable fluorogenic histone deacetylase substrate.
- Produces fluorescent AMC upon deacetylation and proteolytic cleavage.
- Excitation/emission: 355 nm/460 nm for sensitive detection.
- High purity suitable for enzymatic activity and inhibitor assays.
- Soluble in DMSO (≈100 mg/mL); sonication may be required.
- Store sealed, away from moisture and light to maintain stability.
Apexbio Technology LLC Ac-IETD-AFC,5mg CAS# 211990-57-7
Ac-IETD-AFC is a fluorogenic substrate of caspase-8 and possesses amino aicd sequence IETD which is recognized by caspase-8-mediated proteolysis. It is also a substrate for caspase-3 processing enzyme, caspase-10, and granzyme B. Caspase activity can be quantified by fluorescent detection of free AFC at Ex. = 400 nm and Em. = 505 nm. Ac-IETD-AFC can be used to identify and quantify caspase-8 activity in apoptotic cell lysates and to study events downstream of caspase-8 activation. Other sizes are also available. Please inqury us for quote.
Cayman Chemical Abl SubstrAT PeptIdetrIflu 5mg
A peptide substrate for Abl; selectively phosphorylated by Abl over c-Src kinase at 20 µM; has been used to quantify Abl kinase activity in vitro
Bioworld pNPP (p-Nitrophenyl Phosphate), Disodium, hexahydrate, 5 g
Colorimetric alkaline phosphatase soluble substrate, p-Nitrophenyl Phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in Enzyme Linked Immunosorbant Assay (ELISA) procedures. The reaction produces a soluble end product that is yellow in color. Can be used to perform kinetic and endpoint determinations. Also used as a phosphorylating agent in nucleotide synthesis.Specifications:CAS Number: 4264-83-9Molecular Weight: 371.14Molecular Formula: C6H4NO6P•2Na•6H2OPhysical Appearance: White to yellow crystalline powderStorage:Store at 4° C, Unstable in solution; reconstitute just prior to use.
Enzo Life Sciences Ac-WEHD-pNA (5mg)
Colorimetric caspase substrate. Combinatorial studies have found the WEHD sequence to be optimal for clevage of tetrapeptide substrate by caspase-1, caspase-4, caspase-5 and caspase-14. Cleavage of the substrate by a caspase releases pNA, which can be detected by its absorbance at 405nm. Alternative name: Caspase 1 substrate (chromogenic), Caspase 5 substrate (chromogenic). Formula: C34H37N9O11. MW: 747.7. Purity: ≥98% (HPLC). Formulation: Lyophilized. Solubility: Soluble in DMSO. Dilute with buffer, pH 7.5. Long Term Storage: -20°C.
Medchemexpress LLC HER2/CD340 Human B 50ug | 50ug
The HER2/CD340 protein is a dynamic tyrosine kinase that is essential in the neuregulin receptor complex and regulates microtubule dynamics Upon activation it triggers the MEMO1-RHOA-DIAPH1 pathway inhibits GSK3B and promotes the association of APC and CLASP2 on the cell membrane to achieve microtubule stabilization HER2/CD340 Protein Human (Biotinylated Domain 4 HEK293 His-Avi) is the recombinant human-derived HER2/CD340 protein expressed by HEK293 with C-His-Avi labeled tag
Apexbio Technology LLC D-Luciferin (potassium salt) 115144-35-9 1g
D-Luciferin (potassium salt) a potassium salt derivative of D-luciferin serves as the bioluminescent substrate for firefly luciferase The enzyme-mediated oxidation of D-luciferin in the presence of ATP and oxygen produces bioluminescent emission at approximately 560 nm without the need for external excitation This property is applied extensively in bioluminescence imaging (BLI) experiments including live-cell and small-animal imaging Cells genetically engineered to express luciferase utilize D-luciferin to generate optical signals facilitating non-invasive visualization and tracking of biological events such as tumor growth gene expression dynamics in vivo distribution and therapeutic responses
Medchemexpress LLC HER2/CD340 Human B 20ug | 20ug
The HER2/CD340 protein is a dynamic tyrosine kinase that is essential in the neuregulin receptor complex and regulates microtubule dynamics Upon activation it triggers the MEMO1-RHOA-DIAPH1 pathway inhibits GSK3B and promotes the association of APC and CLASP2 on the cell membrane to achieve microtubule stabilization HER2/CD340 Protein Human (Biotinylated HEK293 His) is the recombinant human-derived Her2/CD340 protein expressed by HEK293 with C-His labeled tag
Medchemexpress LLC HY-15926 5g , ONPG 2-Nitrophenyl β-D-galactopyranoside CAS:369-07-3 Purity:98%
Medchemexpress, HY-15926 5g ONPG 2-Nitrophenyl β-D-galactopyranoside CAS:369-07-3 Formula:C12H15NO8 Purity:98% ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity. Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
Ubpbio A fluorogenic substrate of proteasomes, preferentially cleaved by immunoproteasomes.
Ac-ANW-AMC is a fluorogenic substrate of the chymotrypsin-like activity of proteasomes. This substrate is preferentially cleaved by immunoproteasomes compared to constitutive proteasomes. The AMC fluorescence can be detected by a fluorimeter or a plate reader using excitation/emission wavelengths at 360 nm/460 nm, respectively. This substrate can be used to determine the chymotrypsin-like activity of immunoproteasomes. Working concentration is 50 - 200 µM.
AG Scientific Inc IPTG, 1 G
IPTG known as Isopropyl-Beta-D-Thiogalactopyranoside functions as an inducer of galactosidase activity by binding to and inhibiting the repressor.It is utilized for the induction of expression from the lac promoter and derivates. IPTG is also used to differentiate recombinants from non-recombinants in cloning strategies using vectors containing the Z or Z peptide gene.CAS Number: 367-93-1Molecular Weight: 238.3 DaChemical Formula: C9H18O5SSolubility: Soluble in water (5%), renders clear colorless solutionStorage Temperature: -20CResearch or further manufacturing use only, not for food or drug use.
Medchemexpress LLC Z-Ala-Arg-Arg-AMC | 90468-18-1 | 98.4% | 692.77 g/mol | C33H44N10O7 | 5 MG
Z-Ala-Arg-Arg-AMC is a fluorogenic peptide substrate for quantifying trypsin-like activity of the proteasome. Proteolytic cleavage releases 7-amino-4-methylcoumarin (AMC), producing a fluorescence signal used in biochemical and enzymatic assays.
- Generates a fluorescent AMC signal upon proteolytic cleavage.
- Designed for measuring trypsin-like proteasome activity.
- High purity (~98.4%) suitable for research assays.
- Supplied as a solid; soluble in DMSO at about 50 mg/mL with ultrasonic assistance.
- Store sealed, away from moisture, under recommended low-temperature conditions.
- Available in small research-scale quantities (mg scale).
Medchemexpress LLC Recombinant rat HER2 (ErbB2) protein, HEK293-expressed, C-terminal His tag | >95.0% | 50 UG
Recombinant rat HER2 (ErbB2/CD340) protein expressed in HEK293 cells and supplied lyophilized with a C-terminal 10xHis tag. Apparent molecular weight is ~91 kDa, and purity is greater than 95% by reducing SDS-PAGE. Formulated from 20 mM PB, 150 mM NaCl, pH 7.4, it is intended for research applications such as binding assays, antibody validation, and functional studies. Store lyophilized at -20°C and follow reconstitution guidance.
Medchemexpress LLC L-α-Asparagine, N-acetyl-L-tyrosyl-L-valyl-L-alanyl-N-(4-methyl-2-oxo-2H-1-benzopyran- | 149231-65-2 | 99.6% | 665.69 | 25 MG
Ac-YVAD-AMC is a potent caspase inhibitor, commonly used in research to study programmed cell death (apoptosis) pathways. It acts as a fluorogenic substrate for caspase-1, caspase-4, and caspase-5, enabling the detection and quantification of their enzymatic activity in various biological assays.
- Potent caspase inhibitor
- Useful in apoptosis research
- Fluorogenic substrate for caspase-1, caspase-4, and caspase-5
- Enables detection and quantification of caspase activity