Enzymes and Inhibitors

Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.

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991 – 1,005 of 5,313 results

991

Thermo Scientific™ LanthaScreen™ Tb-DUB Substrate

Deubiquitinating enzymes (DUBs) play housekeeping functions by maintaining pools of active ubiquitin, and regulatory function by rescuing specific target proteins from degradation by the proteasome.

Pricing & Availability

992

Promega T4 Polynucleotide Kinase Buffer Pack

T4 PNK catalyzes the transfer of the gamma-phosphate from ATP to the 5'-terminus of polynucleotides or to mononucleotides bearing a 5'-hydroxyl group.

Pricing & Availability

993

Form	Liquid
Color	Colorless

Promega Restriction Enzymes - Spe I Blue/White Cloning and Genome Qualified (B)

Capable of digesting DNA in 15 minutes or less. (A/CTAG T T GATC\A)

Pricing & Availability
Specifications

Cut Site	A.CTAGT TGATC.A
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	SpeI
Storage Buffer	10mM Tris HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
Concentration	10 U/μL
For Use With (Application)	Provides a higher level of quality control for Enzymes used in cloning applications

995

Promega Restriction Enzymes - Not I Blue/White Cloning and Genome Qualified (D)

Capable of digesting DNA in 15 minutes or less. (GC/GGCC GC CG CCGG\CG)

Pricing & Availability
Specifications

Cut Site	GC.GGCCGC CGCCGG.CG
Form	Liquid
Storage Buffer	10mM Tris HCl (pH 7.4), 500mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/mL BSA, 0.1% Triton™ X-100, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
For Use With (Application)	Capable of digesting DNA in <15 min.

996

Elastase is a serine protease that preferentially cleaves at the C-terminus of alanine, valine, serine, glycine, leucine or isoleucine. Elastase has a unique capability of digesting elastin. Elastase activity is optimal at pH 9.0.

Pricing & Availability

997

Promega Klenow Fragment, DNA Polymerase I

A DNA-dependent DNA polymerase that lacks the 5'→3' exonuclease activity of intact E. coli DNA Polymerase I but retains its 5'→3' polymerase, 3'→5' exonuclease and strand displacement activities.

Pricing & Availability
Specifications

Components	10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, 1mM DTT
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	DNA Polymerase I Large (Klenow) Fragment
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Molecular Weight (g/mol)	68
Color	Colorless
pH	7.2
Purity	>80%
For Use With (Application)	Filling-in or labeling 3' ends of 5' overhanging double-stranded DNA, second-strand cDNA synthesis, DNA sequencing by the Dideoxy method
Source	Recombinant E. coli strain

998

Promega RNase One™

Single-strand-specific RNA endonuclease. Cleaves between all ribonucleotides.

Pricing & Availability
Specifications

Components	10X Reaction Buffer: Tris HCl 100mM (pH 7.5 at 25°C), EDTA 50mM, Sodium Acetate 2M
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	Ribonuclease
Storage Buffer	10mM Tris HCl (pH 8), 200mM NaCl, 50% Glycerol
Molecular Weight (g/mol)	27
Color	Colorless
pH	7.3
Concentration	5 to 10 U/μL
For Use With (Application)	Mapping, RNase protection assay, mismatch detection, selective removal of RNA from DNA preparations
Source	Recombinant E. coli strain

999

Promega Alkaline Phosphatase, Calf Intestinal

Alkaline Phosphatase catalyzes the hydrolysis of 5'-phosphate groups from DNA, RNA and ribo- and deoxyribonucleoside triphosphates.

Pricing & Availability
Specifications

Components	10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	Alkaline Phosphatase
Storage Buffer	10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol
Color	Colorless
pH	8
Quantity	1000 U
For Use With (Application)	Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase
Source	Calf intestinal mucosa

1,000

Promega Restriction Enzymes - Mlu I Blue/White Cloning and Genome Qualified (D)

Genome qualified to ensure optimal performance in genomic analysis applications. This Mlul products is active and capable of digestion directly in GoTaq™ Green Master Mix. (A/CGCG T T GCGC\A)

Pricing & Availability

1,001

Promega Restriction Enzymes - Sgf I Genome Qualified (C)

Genome qualified to ensure optimal performance in genomic analysis applications. Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications. (GCG AT/CGC CGC\TA GCG)

Pricing & Availability

1,002

Promega Restriction Enzymes - Nco I Blue/White Cloning Qualified (D)

Capable of digesting DNA in 15 minutes or less. (C/CATG G G GTAC\C)

Pricing & Availability
Specifications

Cut Site	C.CATGG GGTAC.C
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	NcoI
Storage Buffer	10mM Tris HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
Concentration	10 U/μL
For Use With (Application)	Provides a higher level of quality control for Enzymes used in cloning applications

1,003

Promega Arg-C Sequencing Grade Modifying Enzyme

Arg-C (clostripain) is an endopeptidase that cleaves at the C-terminus of arginine residues, including the sites next to proline. Cleavage also will occur at lysine residues. Arg-C activity is optimal in the pH range of 7.6-7.9.

Pricing & Availability

1,004

Promega Exonuclease III

Exonuclease III is a 3' → 5' exonuclease specific for dsDNA.

Pricing & Availability
Specifications

Components	10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2
Content And Storage	-30°C to -10°C
Inhibitors	50mM NaCl
Form	Liquid
Product Type	Exonuclease III
Storage Buffer	20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol
Molecular Weight (g/mol)	28000
Color	Colorless
pH	7.5
Purity	>90%
Concentration	150 to 200 U/μL
For Use With (Application)	Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA
Source	Recombinant E. coli strain

1,005

Promega DNA Polymerase I

DNA Polymerase I catalyzes the template-directed polymerization of nucleotides into duplex DNA in a 5'→3' direction. Recombinant.

Pricing & Availability
Specifications

Components	10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, DTT 1mM
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	DNA Polymerase I
Storage Buffer	50mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 0.1mM EDTA, 50% Glycerol
Color	Colorless
pH	7.5
Purity	>90%
Concentration	5 to 10 U/μL
For Use With (Application)	Labeling of DNA to high specific radioactivity by nick translation, second-strand cDNA synthesis
Source	Recombinant E. coli strain

Enzymes and Inhibitors

Thermo Scientific™ LanthaScreen™ Tb-DUB Substrate

Promega T4 Polynucleotide Kinase Buffer Pack

Promega Caspase Inhibitors

Promega Restriction Enzymes - Spe I Blue/White Cloning and Genome Qualified (B)

Promega Restriction Enzymes - Not I Blue/White Cloning and Genome Qualified (D)

Promega Elastase

Promega Klenow Fragment, DNA Polymerase I

Promega RNase One™

Promega Alkaline Phosphatase, Calf Intestinal

Promega Restriction Enzymes - Mlu I Blue/White Cloning and Genome Qualified (D)

Promega Restriction Enzymes - Sgf I Genome Qualified (C)

Promega Restriction Enzymes - Nco I Blue/White Cloning Qualified (D)

Promega Arg-C Sequencing Grade Modifying Enzyme

Promega Exonuclease III

Promega DNA Polymerase I

Enzymes and Inhibitors

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