Modifying Enzymes
Cayman Chemical N-Oleoyl GlycIn 100mg
An endogenous lipoamino acid; increases triglyceride levels in 3T3-L1 adipocytes, an effect that can be reversed by SR141716; selectively induces the expression of mRNA encoding the CB1 receptor over mRNA encoding the CB2 receptor in the same cells at 50 µM; potentiates glycine-induced chloride currents in Xenopus oocytes expressing α1-, α1β-, α2-, α2β-, or α3 subunit-containing glycine receptors but not those expressing α3β subunit-containing glycine receptors at 1 µM; increases c-Fos protein levels, intracellular calcium levels, and AgRP secretion in mHypoE-38 cells, effects that can be reversed by AM251; reduces binge-like ethanol intake in alcohol-preferring mice without affecting total fluid intake and does not affect sucrose intake in the sucrose preference test in mice at 60 mg/kg; reduces balance impairments, thermal hyperalgesia, mechanical allodynia, aggressiveness, and immobility time in the tail suspension test in a mouse model of traumatic brain injury at 50 mg/kg
Medchemexpress LLC Lipase from Pseudomonas fluorescens (immobilized) | 9001-62-1 | MFCD00131509 | 1g
Lipase Pseudomonas fluorescens (Immobilized) comes from Pseudomonas fluorescens and shows good applicability in ester synthesis of food industry the biofuel synthesis the synthesis of enantiomeric pure chiral drugs and so on[1]
Aldevron Guanylyltransferase, 1750ug
1750ug dispense of Guanylyltransferase. Guanyltransferase (GT), also known as Vaccinia Capping Enzyme (VCE), catalyzes addition of 7-methylguanylate cap structures (Cap 0) to the 5’ end of RNA in three steps:
Reactions 1 & 2: Conversion of an RNA substrate pppN(pN)n to GpppN(pN)n with increasing amounts of enzyme was visualized by gel electrophoresis (uncapped, lower band; capped, upper band). One unit (U) is the amount of enzyme activity that caps 50% of a 5’-ppp oligo in 30 minutes at 37°C.
Reaction 3: Methyl transfer from SAM to GpppN(pN)n producing SAH and cap-0 mRNA was measured using the EPIgeneousTM Methyltransferase Assay (Cisbio). One unit (U) is the amount of enzyme activity that produces 1 pmol of SAH from SAM in 30 minutes at 37°C using cap-0 substrate.
Echelon Biosciences Research Labs GLO-PI(3,4,5)P3 BODIPYTMR50ug
BODIPY TMR PI(3,4,5)P3 (BODIPY TMR Phosphatidylinositol 3,4,5-trisphosphate) is a water soluble analog of PI(3,4,5)P3 (PIP3) labeled with the orange fluorophore, Bodipy-TMR, at the sn-1 position.Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 3,4,5-trisphosphate (PIP3), formed from PI(4,5)P2 though phosphorylation by PI 3-kinase, activates numerous signaling pathways resulting in cell proliferation, growth, survival, glucose transport and protein synthesis. High PIP3 levels from disregulation of PI3-K have been demonstrated in cancer and inflammatory diseases. PIP3 is hydrolyzed by the phosphatases PTEN to PI(4,5)P2 and SHIP to PI(3,4)P2. This item is non returnable
LifeSensors USP15 25µg
USP15 (Ubiquitin Specific Protease 15, UNPH4) is a deubiquitinase that associates with the COP9 signalosome (CSN) complex. It regulates various pathways such as the TGF-beta receptor signaling, NFκB and RNF41/NRDP1-PRKN pathways.
Carnabio Usa Inc AMPKA1/B1/G1(PRKAA1/B1/G1)/10U
AMPK1/1/1(PRKAA1/B1/G1), Full-length, Wild type, Amino Acid 1-550, NP_006242.4, Expressed in Insect (sf21); 10microg
Carnabio Usa Inc WNK3/10UG/CATALYTIC
WNK3, Catalytic domain, Wild type, Amino Acid 1-434, NP_065973.2, Expressed in Insect (sf21); 10microg
ABclonal Technology T7 DNA Ligase
T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7. It can catalyze the formation of a phosphodiester bond between adjacent 5 phosphate and 3 hydroxyl termini in double-stranded DNA. T7 DNA ligase can catalyze ligation of cohesive ends and repair nicks, but not blunt end ligation. This makes a good choice for applications in which blunt and cohesive ends of DNA are present but only the cohesive ends are to be joined .
Sigma Aldrich Fine Chemicals Biosciences Catalase from human erythrocytes >=90% (SDS-PAGE), buffered aqueous solution, >=30,000 units/mg protein | 9001-05-2 | MFCD00081483 | 0.5MG
Catalase from human erythrocytes >=90% (SDS-PAGE), buffered aqueous solution, >=30,000 units/mg protein | Purity: >=90% (SDS-PAGE) | 9001-05-2 | MFCD00081483 | 0.5MG
Cayman Chemical Oleoyl SerotonIn-d17 500UG
An internal standard for the quantification of oleoyl serotonin by GC- or LC-MS
Cayman Chemical Oleoyl SerotonIn-d17 100UG
An internal standard for the quantification of oleoyl serotonin by GC- or LC-MS
Enzo Life Sciences PARP-1 (catalytic domain) (human), (recombinant) (His-tag) (20µg)
Alternative name: Poly(ADP-ribose) polymerase 1. Purity: ≥90% (SDS-PAGE). MW: ~46kDa. Formulation: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 300mM sodium chloride, 250mM imidazole, 2mM β-mercaptoethanol, 0.2% NP-40 and 10% glycerol. Source: Produced in SF21 insect cells. Human PARP-1 (aa 656-1014) is fused to a C-terminal His-tag. Produced in a baculovirus expression system. Long Term Storage: -80°C. Handling: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Sigma Aldrich Fine Chemicals Biosciences Ribonuclease H RNase H fro
Ribonuclease H (RNase H) is a nonspecific endoribonuclease localized to the nucleus and cytoplasm. It is ubiquitously found and widely present among many organisms including viruses and human.
Medchemexpress LLC Vu0360172 | 1310012-12-4 | 98.1% | 5 MG
Small-molecule positive allosteric modulator of the metabotropic glutamate receptor 5 (mGlu5) supplied for research use in small milligram quantities.
- High purity (98.1%).
- Molecular weight 294.32 g/mol.
- Molecular formula C18H15FN2O.
- Suitable for in vitro pharmacology and receptor modulation studies.
- Available in small milligram sizes for assay development.
Aldevron 2'O-Methyltransferase, 3400ug
3400ug dispense of 2'O-Methyltransferase. 2'-O-Methyltransferase adds a methyl group to the 2'-OH of the first nucleotide adjacent to the m7Gppp5'N cap. 2'-O-Methyltransferase utilizes S-adenosylmethionine as the methyl group donor.