Modifying Enzymes
Agilent Technologies PFUTURBO DNA POLYMERASEPFUTUR
PfuTurbo DNA Polymerase, 500U (200 rxn), Contents: PfuTurbo DNA polymerase 10X cloned Pfu buffer, Applications: High performance PCR, For high fidelity PCR and primer-extension reactions, PCR cloning and generating blunt-end amplification p
New England Biolabs, Inc. Human Alkyladenine Glycosylase (hAAG) – 500 units
hAAG excises alkylated and oxidative DNA damaged sites and catalyzes the hydrolysis of the N-glycosidic bond to release the damaged base.
Worthington Biochemical Corporation DIAPHORASE 1 KU
Diaphorase, Code: DILW, Store at 2 deg. to 8 deg.C, Minimum activity: 30U/mg dry weight, Supplied as a dialyzed, lyophilized powder, Unit Definition: 1 Unit equals a decrease in absorbance at 600nm of 1.0 per minute at 25 deg.C, pH 7.5, 1kU
New England Biolabs, Inc. Pyrophosphatase, Inorganic (E. coli) – 50 units
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate:
Sigma Aldrich Fine Chemicals Biosciences M-MLV Reverse Transcriptase Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis | 9068-38-6 | MFCD00283754 | 40KU
M-MLV Reverse Transcriptase Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis | 9068-38-6 | MFCD00283754 | 40KU
Worthington Biochemical Corporation DNASE 11MG FILTER
Filtered through a 0.22 micron membrane and lyophilized in vials. Material is not tested for pyrogenicity.
Sino Biological Recombinant DNase I (RNase-free) 1000 U
DNase I is an endonuclease that can non-specifically cleave DNA, producing dinucleotide, trinucleotide, and oligonucleotide products with 5 ́ phosphorylated and 3 ́ hydroxylated ends. DNase I acts on single-and double-stranded DNA, chromatin, and RNA:DNA hybrid strands. Certified by highly sensitive fluorescence probe assays, our DNase I (RNase-free) demonstrates virtually undetectable RNase activity-a level of purity that surpasses other commercial sources. Trust in this "Ultra-Pure" standard for the ultimate reliability in your RNase-sensitive workflows.
Abcam Dyngo 4a, Novel, highly potent dynamin inhibitor, 5MG
Dyngo 4a, Novel, highly potent dynamin inhibitor (IC50 values are 400 nM and 200 nM at dynamin I and II human-recombinant, respectively). Inhibits clathrin-mediated endocytosis (IC50 = 5.5 µM). Dynasore (ab120192) analog. Prevents botulin neurotoxin A (BoNTs) uptake and internalization, and prevents SNAP25 cleavage. Inhibits BoNTs-induced paralysis and delays botulism onset. - Cited in over 75 publications - Purity > 99% - Soluble in DMSO to 100 mM
The product is subject to the following: Abcam Restricted Use Statement
AMID BIOSCIENCES Amid Biosciences, T4 RNA Ligase 2, Recombinant, RNA Ligation, 1000 Units
T4 RNA Ligase 2 is supplied in a 1000 Units format for RNA ligation workflows that require enzymatic joining of RNA substrates or adapter containing molecules. Applications include Intermolecular and intramolecular RNA strand joining, nick ligation on double stranded RNA, RNA-DNA hybrid ligation, ligation of 3 OH RNA to 5 phosphate DNA in duplex structures. This product includes 1mL of 10X Reaction Buffer, 100 mM Tris-HCl, pH 7.5, 20 mM MgCl2, 20 mM DTT, 5 mM ATP. Requires ATP unless the substrate is preadenylated. Technical specifications are included below.- Quantity - 1000 Units- Organism - Bacteriophage T4- Activity - 10,000 U/mL (greater than 40,000 U/mg)- Buffer - 10 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1 mM DTT, 0.1 mM EDTA, 50% Glycerol- Storage - -20 CThis product is for research use only.
Sigma Aldrich Fine Chemicals Biosciences Beta-Glucuronidase from Helix pomatia | 9001-45-0 | MFCD00130629 | 1 million units
Beta-Glucuronidase from Helix pomatia | 9001-45-0 | MFCD00130629 | 1 million units
New England Biolabs, Inc. Electroligase – 50 reactions
ElectroLigase combines T4 DNA ligase and an optimized, ready-to-use 2X reaction buffer containing a proprietary ligation enhancer and no PEG. This combination is specifically formulated to promote robust ligation of all types of DNA ends (blunt, sticky, TA). It is directly compatible, without desalting or purification, with electrocompetent cells used for transformation by electroporation. No thawing of the buffer is required as it maintains a liquid state during storage at -20C, thereby simplifying reaction set-up. By providing an optimized ratio of enzyme and buffer components, users are able to rapidly ligate all types of DNA ends applying a short incubation time at room temperature. Ligations for subcloning can be carried out in small volumes with low concentrations, allowing users to conserve precious DNA samples. These reactions can be pipetted directly, without purification or dilution, to transform many strains of electrocompetent E. coli.
Supply Solutions RNase A, 25 mg; from bovine pancreas (Roche); pkg of 100 mg (10109169001) pkg of 25 mg (10109142001)
RNase A, 25 mg; from bovine pancreas (Roche); pkg of 100 mg (10109169001) pkg of 25 mg (10109142001)
Agilent Technologies GLYKO SIALIDASE ASAMPLER KIT
Try our lower MW isoforms for challenging sialidase digests.This Sampler Kit contains 1 vial each of 3 isoforms (88, 66 and 51 kDa) of the enzyme Sialidase A. Lower MW sialidases may help desialylate glycans if steric hindrance is an issue with regular sialidase.
LGC GENOMICS LLC (Lucigen) Ampligase DNA Ligase without Buffer, 10,000 U @ 100 U/LHighly thermostable DNA ligase for applications where ligation at high temperature is beneficial or required.
Ampligase Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3-hydroxylated and 5-phosphorylated termini in duplex DNA structures that are stable at high temperaturesHigh thermostability allows ligation using high-stringency hybridization conditionsHigh specificity and stringency permits sensitive detection of SNPsAvailable in kit and "enzyme only" formats11
Sigma Aldrich Fine Chemicals Biosciences Proteinase K from Tritirachium album, 39450-01-6, MFCD00132129, 25 mg
Lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology, Synonym: Endopeptidase K. Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.