Modifying Enzymes
Thermo Scientific™ DNase I, RNase-free (supplied with MnCl2) (1 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Thermo Scientific™ DNase I, RNase-free, HC (50 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)
Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.
Thermo Scientific™ T4 DNA Ligase, LC (1 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Invitrogen™ SUMO Protease
A recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae
Invitrogen™ CorrectASE™ Enzyme
CorrectASE™ enzyme removes mismatches caused by oligonuceotide synthesis errors, leading to a 3–10 fold reduction in mutations in your synthetic genes or fragments.
Invitrogen™ Proteinase K, recombinant
Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.
Thermo Scientific™ Modifying Enzymes
Thermo Scientific enzymes for in vitro transcription are designed for efficient and reliable RNA synthesis. These enzymes cover the entire synthesis workflow from template preparation to post-transcriptional modifications, ensuring optimal performance and high yields of synthesized RNA.
Invitrogen™ Terminal Deoxynucleotidyl Transferase (TdT) Buffer
Supplied with Terminal Deoxynucleotidyl Transferase (TdT)
Invitrogen™ T4 DNA Ligase (1 U/μL)
Ideal for cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA
Thermo Scientific™ Terminal Deoxynucleotidyl Transferase (20 U/μL)
Terminal Deoxynucleotidyl Transferase (TdT) catalyzes the template-independent addition of deoxyribonucleotides to the 3'-OH terminus of DNA molecules.
MP Biomedicals™ Hyaluronidase, Bovine Testes
Bovine Testes
Invitrogen™ CorrectASE™ Enzyme
CorrectASE™ enzyme removes mismatches caused by oligonuceotide synthesis errors, leading to a 3–10 fold reduction in mutations in your synthetic genes or fragments.