Modifying Enzymes
New England Biolabs, Inc. NotI-HF® – 500 units
A high fidelity restriction endonuclease that recognizes the sequence GC^GGCC_GC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
- Reduced star activity
- Supplied with 1 vial of Gel Loading Dye, Purple (6X)
New England Biolabs, Inc. Uracil-DNA Glycosylase (UDG) – 5000 units
E. coli Uracil-DNA Glycosylase (UDG) catalyses the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases).
New England Biolabs, Inc. Fpg – 2500 units
Fpg (formamidopyrimidine [fapy]-DNA glycosylase) (also known as 8-oxoguanine DNA glycosylase) acts both as a N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged purines from double stranded DNA, generating an apurinic (AP site). The AP-lyase activity cleaves both 3' and 5' to the AP site thereby removing the AP site and leaving a 1 base gap. Some of the damaged bases recognized and removed by Fpg include 7, 8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, fapy-guanine, methy-fapy-guanine, fapy-adenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine and 5-hydroxy-uracil.
New England Biolabs, Inc. SP6 RNA Polymerase – 2000 units
Bacteriophage SP6 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the SP6 phage promoter. The 98.5 KD polymerase catalyzes in vitro RNA synthesis from a cloned DNA template under the SP6 promoter. RNA synthesized using the SP6 RNA Polymerase is suitable for many applications in research and biotechnology. Reaction Conditions: 1X RNAPol Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP and DNA template containing the SP6 RNA Polymerase promoter. Incubate at 37°C.
New England Biolabs, Inc. ShortCut RNase III – 200 units
ShortCut RNase III, used with its manganese-containing reaction buffer, converts long double-stranded RNA into a heterogeneous mix of short (18-25 bp) interfering RNAs (siRNA) suitable for RNA interference in mammalian cells. 1.5 units (1 µl) of ShortCut RNase III is sufficient to convert 1µ g of dsRNA into siRNA suitable for RNA interference in mammalian cells.
New England Biolabs, Inc. T4 DNA Ligase – 100,000 units – 2,000,000 units/ml
The industry standard for performance and quality. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.
- Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA
- Joins blunt and cohesive end termini as well as repair single stranded nicks in duplex DNA
- T4 DNA Ligase variants: Salt-T4, Hi-T4, Instant Sticky-end Ligase MM, Blunt/TA Ligase MM
New England Biolabs, Inc. Exonuclease I (E.coli) – 3000 units
Exonuclease I is a DNA-specific endonuclease that catalyzes the removal of nucleotides from linear single-stranded DNA in the 3' to 5' direction. Note: See also Exo-CIP Rapid PCR Cleanup Kit.
New England Biolabs, Inc. T4 DNA Ligase – 20,000 units – 2,000,000 units/ml
The industry standard for performance and quality. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.
- Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA
- Joins blunt and cohesive end termini as well as repair single stranded nicks in duplex DNA
- T4 DNA Ligase variants: Salt-T4, Hi-T4, Instant Sticky-end Ligase MM, Blunt/TA Ligase MM
New England Biolabs, Inc. Exonuclease I (E.coli) – 15000 units
Exonuclease I is a DNA-specific endonuclease that catalyzes the removal of nucleotides from linear single-stranded DNA in the 3' to 5' direction. Note: See also Exo-CIP Rapid PCR Cleanup Kit.
New England Biolabs, Inc. Klenow Fragment (3'-5' exo-) – 200 units
Klenow Fragment (3' to 5' exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5' to 3' exonuclease activity and has mutations (D355A, E357A) which abolish the 3' to 5' exonuclease activity.
New England Biolabs, Inc. USER® Enzyme – 50 units
USER (Uracil-Specific Excision Reagent) Enzyme generates a single nucleotide gap at the location of a uracil. USER Enzyme is a mixture of Uracil DNA glycosylase (UDG) and the DNA glycosylase-lyase Endonuclease VIII. UDG catalyses the excision of a uracil base, forming an abasic (apyrimidinic) site while leaving the phosphodiester backbone intact. The lyase activity of Endonuclease VIII breaks the phosphodiester backbone at the 3 and 5 sides of the abasic site so that base-free deoxyribose is released.Applications of USER include:
- Directional RNA Seq
- NEBNext adaptor cleavage
- USER cloning
New England Biolabs, Inc. α1-6 Mannosidase - 800 units
a1-6 Mannosidase is a highly specific exoglycosidase that removes unbranched a1-6 linked mannose residues from oligosaccharides. When used in conjunction with a1-2,3 Mannosidase, the a1-6 Mannosidase will cleave a1-6 mannose residues from branched carbohydrate substrates.
New England Biolabs, Inc. Enterokinase, light chain – 480 units
Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate.
New England Biolabs, Inc. Lambda Protein Phosphatase – 100000 units
Lambda Protein Phosphatase is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues.
New England Biolabs, Inc. MscI – 1250 units
A restriction endonuclease that recognizes the sequence TGG_^CCA.