Modifying Enzymes
Ubpbio HUMAN UBE1, 25UG
E1 Activating Enzymes – UbE1 (UBPBio catalog# B1100, B1101) – Purified from sf9 insect cells. UBE1 is an E1 enzyme within the E1, E2, E3 cascade which conjugates Ub to protein substrates. UBE1 activates Ub by catalyzing an ATP – dependent reaction; Ub is conjugated onto the catalytic Cys residue of UBE1 by formation of a thioester bond. The activated Ub is then passed onto a Ub-conjugating enzyme E2; Ub charged E2 binds a Ub ligase E3 that directly binds substrate proteins. Ub is transferred to the HECT domain E3s prior to being conjugated on substrate proteins. For RING domain E3s, Ub is transferred directly from an E2 to substrate proteins.
ACROBiosystems Endoglycosidase H (Endo H) is a recombinant glycosidase cloned from Streptomyces plicatus and overexpressed in E. coli. Endo H cleaves the chitobiose core of high-mannose oligosaccharides and a limited number of hybrid oligosaccharides from
Endoglycosidase H is a recombinant glycosidase which cleaves within the chitobiose core of high mannose and some hybrid oligosaccharides from N-linked glycoproteins. It is a recombinant glycosidase cloned from Streptomyces plicatus and overexpressed in E. coli.
New England Biolabs, Inc. Bst DNA Polymerase, Large Fragment – 8000 units
Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5' to 3' polymerase activity, but lacks 5' to 3' exonuclease activity.
New England Biolabs, Inc. Taq DNA Ligase Reaction Buffer – 6 ml
This is the NEB reaction buffer supplied with Taq DNA Ligase. Taq DNA Ligase requires NAD+ as a cofactor which is incorporated into the buffer. New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
INTACT GENOMICS INC RNase Inhibitor, Murine (Glycerol-free) 20,000 Units
Intact Genomics (ig) Glycerol-free RNase Inhibitor is a 49.8 kDa recombinant protein of murine origin which inhibits RNases A, B and C by binding noncovalently in a 1:1 ratio with high affinity. Moreover, it does not inhibit polymerase activity when used with Taq DNA Polymerase, Reverse Transcriptase. Glycerol-free RNase Inhibitor from Murine is resistant to oxidation compared to the human/porcine RNase inhibitors as it lacks a pair of cysteine residues found in the human/porcine versions. Stable at less than 1 mM DTT.Benefits of Glycerol-Free Enzyme: Optimal for Lyophilization ensuring stability and performance for point-of-care tests like molecular diagnostics. Better Compatibility: Avoids interference. Improved Performance: Enzyme formulations are less viscous. Simplified Integration: Allows for more flexible formulation.Applications: cDNA synthesis RT-PCR/RT-qPCR In vitro transcription RNA labeling Microfluidics assays
New England Biolabs, Inc. AluI Methyltransferase – 100 units
AluI Methyltransferase modifies the cytosine residue (C5) of the sequence AGCT.
New England Biolabs, Inc. α1-2 Fucosidase - 1000 units
a1-2 Fucosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of linear 1-2 linked fucose residues from oligosaccharides
Sigma Aldrich Fine Chemicals Biosciences T4 DNA Ligase recombinant
T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3-hydroxyl- and 5-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.ContentsT4 DNA Ligase supplied with 10x concentrated ligation buffer that includes ATP.
Sigma Aldrich Fine Chemicals Biosciences Isovaleryl coenzyme A lith10MG
Isovaleryl coenzyme A an intermediate of the leucine catabolism. It is synthesized from -methylbutyric acid by the action of enzyme acyl CoA synthetase. The conversion of isovaleryl-CoA to methylcrotonyl-CoA is catalyzed by the enzyme isovaleryl-CoA dehydrogenase in leucine catabolism pathway. The enzyme isovaleryl-CoA oxidase also calalyzes this conversion in fatty acid -oxidation pathway.
Sigma Aldrich Fine Chemicals Biosciences Topoisomerase II alpha hum
Topoisomerase II is encoded by TOP2A gene which is located on 17q21.2 in the human chromosome. It is essential for controlling the topological state of DNA by reversibly breaking the DNA strands during replication recombination and transcription. TOP2A expression increases at late S phase and during G2/M of the cell cycle and decreases after mitosis. Polymorphism with TOP2A causes chromosome instability and tumorigenesis. TOP2A causes tumor proliferation and invasiveness in various cancer types like breast cancer ovarian cancer and prostate cancer.
Sigma Aldrich Fine Chemicals Biosciences N Glycosidase f PNGase f o
Peptide-N-glycosidase f Peptide-N4-(acetyl-beta-glucosaminyl)-asparagine amidase