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DNA Vectors

DNA molecules used to transfer foreign genetic material into another cell, where it can be replicated and/or expressed. Products include cloning kits, transfection kits, expression kits, assay kits, and a variety of sequenced plasmids.
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331 – 345 of 1,808 results
331

Promega BTK-NanoLuc Fusion Vector, 20microg

The NanoLuc Kinase Fusion Vectors contain the coding region of the indicated human kinase fused to either the C- or the N-terminus of NanoLuc luciferase. Designed for use with the NanoBRET Target Engagement (TE) Intracellular Kinase Assay.

ENCOMPASS
332

Promega DDR1-NanoLuc Fusion Vector, 20microg

The NanoLuc Kinase Fusion Vectors contain the coding region of the indicated human kinase fused to either the C- or the N-terminus of NanoLuc luciferase. Designed for use with the NanoBRET Target Engagement (TE) Intracellular Kinase Assay.

ENCOMPASS
333

Promega pGEM™ Express Positive Control Template

The pGEM Express Positive Control Template is used to monitor in vitro transcription reactions when using the Riboprobe Systems.

ENCOMPASS
334

Promega pTNT™ Vector

The pCMVTNT and pTNT Vectors are designed for convenient expression of cloned genes in vitro or in vivo. SP6 and T7 promoters allow expression from SP6- or T7-based coupled in vitro transcription/translation systems.

ENCOMPASS
335

Promega pCI and pCI-neo Mammalian Expression Vectors

Contains the CMV immediate-early enhancer/promoter region and is designed to promote constitutive expression of cloned DNA inserts in mammalian cells.

ENCOMPASS
Promoter CMV
Content And Storage -30°C to -10°C
Storage Buffer TE Buffer (pH 8)
Quantity 20 μg
For Use With (Application) Promote constitutive expression of cloned DNA inserts in Mammalian cells, can also be used to synthesize transcripts in-vitro using the T7 RNA Polymerase promoter or generate single-stranded DNA in E. coli using the f1 origin of replication
336

Promega pGL4.21[luc2P/Puro] Luciferase Reporter Vector

The Vectors is optimized for expression in mammalian cells.

ENCOMPASS
337

Promega pGL4.19[luc2CP/Neo] Luciferase Reporter Vector

The Vector offers standard and destablized luciferase with neomycin for stable selection and is optimized for expression in mammalian cells.

ENCOMPASS
338

Promega psiCHECK™ Vectors

psiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference assays.

ENCOMPASS
339

Promega pGL4 Minimal Promoter Driven Luciferase Vectors

Firefly luciferase reporter vectors with response elements.

ENCOMPASS
Promoter SV40
Content And Storage -30°C to -10°C
Storage Buffer 10mM Tris HCl (pH 7.4), 1mM EDTA
Primer RV Primer 3 and RV Primer 4
Protein Degradation Sequence hPEST
Quantity 20 μg
Concentration 1 μg/μL
For Use With (Application) Transcription regulation, virus-cell interactions, compound screening, post-translational modifications, GPCR signaling, cell signaling, promoter analysis, transient transfection experiments
Reporter Gene luc2P
Marker Gene Hygro
340

Promega Monster Green™ Fluorescent Protein phMGFP Vector

The Monster Green Fluorescent Protein encoded by the hMGFP gene is an ideal fluorescent reporter, providing high-level fluorescence and reducing cytotoxicity.

ENCOMPASS
341

Promega Nano-Glo HiBiT Extracellular Detection System, 10ml

Nano-Glo HiBiT Extracellular Detection System quantitates cell surface or secreted protein expression using a simple add-mix-read assay format and the nonlytic Nano-Glo HiBiT Extracellular Detection Reagent.

ENCOMPASS
342

Promega HaloTag™ Vectors

A variety vectors for mammalian, E. coli, and cell-free protein expression

ENCOMPASS
Content And Storage -30°C to -10°C
For Use With (Application) Cell imaging of Protein localization or trafficking in conjunction with the fluorescent HaloTag™ ligands by using expression of HaloTag™ fusion Proteins in E. coli and cell-free Protein expression
343

Promega pBiT3.1-N[CMV/HiBiT/Blast] Vector, 20microg

The pBiT3.1-N, pBiT3.1-C and pBiT3.1-secN [CMV/HiBiT/Blast] Vectors are traditional cloning vectors for inserting proteins of interest. Cloned ORFs contain the HiBiT tag at the N or C terminus; the N terminus for transmembrane or secreted proteins.

ENCOMPASS
344

Promega pRL Family of Renilla Luciferase Control Reporter Vectors

The pRL Vectors are wildtype Renilla luciferase control reporter vectors, which provide constitutive expression of Renilla luciferase and can be used in combination with a firefly luciferase vector to cotransfect mammalian cells.

ENCOMPASS
Content And Storage -30°C to -10°C
Storage Buffer TE Buffer (pH 7.4)
Quantity 20 μg
For Use With (Application) Provide constitutive expression of Renilla reniformis (sea pansy) Luciferase, can be used in combination with a firefly Luciferase vector to co-transfect mammalian cells
Reporter Gene Luciferase
345

Promega pmirGLO Dual-Luciferase miRNA Target Expression Vector

The pmirGlo™ Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3' of the firefly luciferase gene (luc2).

ENCOMPASS