Protein Analysis Reagents
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Invitrogen™ MAX Efficiency™ DH5αF'IQ Competent Cells
MAX Efficiency DH5αF'IQ Competent Cells are high transformation efficiency chemically competent cells prepared by a patented modification of the procedure of Hanahan. This strain is a derivative of the DH5α strain, sharing the same benefits.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Cytiva HisTrap™ excel Prepacked Columns
For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.
| Type | Pre-packed Column |
|---|---|
| Content And Storage | 4°C to 30°C |
| Description | HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). |
| Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
| pH Range | 2 to 12 |
| Material | Polypropylene |
| Support Type | 6% Highly Cross-linked Agarose |
Cytiva Ni Sepharose™ 6 Fast Flow
Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.
| Particle Size | 90μm |
|---|---|
| Buffer | 0.1M HCl, 0.1M NaOH, 8M Urea |
| Flow Rate | 600 cm/hr. |
| Height (Metric) | 250mm |
| Content And Storage | 4° to 30°C |
| pH Range | 3 to 12 |
| Product Type | Ni Sepharose 6 FF chromatography media |
| dimMatrix | Ni-Sepharose |
| Support Type | 6% Cross-linked Agarose |
| Max. Pressure | 1 bar |
| Height (English) | 9.84 in. |
Cytiva Ni Sepharose™ excel
Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
| Particle Size | 90μm |
|---|---|
| Buffer | 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol |
| Flow Rate | 150 to 600cm/hr. |
| Content And Storage | 4° to 30°C |
| pH Range | 2 to 12 |
| Product Type | Ni Sepharose excel chromato graphy media |
| dimMatrix | Ni-Sepharose |
| Support Type | 6% Highly Cross-linked Agarose |
Tocris Bioscience™ Deschloroclozapine
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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High affinity and highly potent activator of hM3Dq and hM4Di DREADDs; rapidly blood brain barrier penetrant
| Target | DREADD Activators |
|---|---|
| CAS | 7-7-1977 |
| Purity | 0.98 |
| Chemical Name or Material | 11-(4-Methyl-1-piperazinyl)-5 H-dibenzo[b,e][1,4 ]diazepine |
| Recommended Storage | Store at RT |
| Molecular Formula | C18H20N4 |
Thermo Scientific™ Pierce™ ChIP-Grade Protein A/G Plus Agarose
Chromatin immunoprecipitation (chromatin IP, ChIP) assay kit containing all reagents, agarose affinity beads, and a simple, fast, reliable protocol.
| Stationary Phase | Protein A/G |
|---|---|
| Type | Agarose |
| Target | IgG Antibody |
| Content And Storage | Upon receipt store at 4°C |
| Format | Bottle |
| Form | Liquid Suspension |
| Column Type | Affinity, Agarose Resin |
| Product Line | Pierce |
Cytiva Disposable PD-10 Columns
Use for gravity flow purification. Disposable PD-10 Columns are economical, plastic columns that can be packed with a wide range of bulk media.
Cytiva HisTrap™ FF Columns: 16 x 25mm
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
| Flow Rate | <20 mL/min. |
|---|---|
| Type | Pre-packed Column |
| Content And Storage | 4°C to 30°C, 20% Ethanol |
| Diameter (English) Inner | 0.6 in. |
| Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
| Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
| pH Range | 3 to 12 |
| Diameter (Metric) Inner | 16 mm |
| Material | Polypropylene |
| Support Type | 6% Cross-linked Agarose |
Cytiva PD SpinTrap™ G-25 Desalting Columns
Use for convenient and fast small-scale cleanup of proteins and other large biomolecules (>5000 Mr). Cytiva Life Sciences™ PD SpinTrap™ G-25 Desalting Columns provide the option of running multiple samples in parallel.
Cytiva HiTrap™ SP Sepharose Fast Flow IEX Columns
Use for small-scale protein purifications Cytiva Life Sciences™ HiTrap™ SP Sepharose Fast Flow IEX Columns are strong cation exchanger columns for rapid separations either alone or connected in series.
| Content And Storage | 4°C to 30°C, 0.2M Sodium Acetate in 20% Ethanol |
|---|---|
| Description | HiTrap SP FF |
Cytiva HiTrap™ NHS-Activated HP Columns
Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.
| Particle Size | 34 μm |
|---|---|
| Ligand Type | 10μM NHS groups/mL medium |
| Height (Metric) | 2.5cm |
| Content And Storage | 4° to 8°C |
| pH Range | 3 to 12 |
| Product Type | NHS-Activated HP Column |
| For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
| Material | Polypropylene |
| For Use With (Application) | Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines |
| Support Type | 6% Highly Cross-linked Agarose |
| Max. Pressure | 42psi |
| Height (English) | 0.98 in. |
Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
| Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
|---|---|
| Type | Pre-packed Column |
| Content And Storage | 4°C to 30°C |
| Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
| Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
| pH Range | 3 to 12 |
| Material | Polypropylene |
| Support Type | 6% Highly Cross-linked Agarose |
Cytiva Streptavidin Sepharose™ High Performance Medium
Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium.
Cytiva His GraviTrap™ Columns
Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.
Cytiva HiTrap™ Heparin HP Columns
Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.
| Particle Size | 34 μm |
|---|---|
| Ligand Type | 10mg Heparin/mL medium |
| Height (Metric) | 2.5cm |
| Content And Storage | 4° to 30°C |
| pH Range | 5 to 10 |
| Product Type | Heparin HP Column |
| For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
| Material | Polypropylene |
| For Use With (Application) | Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins |
| Support Type | 6% Highly Cross-linked Agarose |
| Max. Pressure | 42psi |
| Height (English) | 0.98 in. |