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A wide variety of assay products for identifying and analyzing of proteins and their functions. Products utilize various detection methods and sample types and can be used for applications including medical, cellular, molecular, and protein research.
Generate C-Terminal Fusions to NanoLuc™ Luciferase Reporter. The pFC32A and pFC32K Nluc CMV-neo Flexi™ Vectors use a directional cloning method based on two rare-cutting restriction enzymes, SgfI and PmeI, and contain a mammalian selectable marker to create a stable line.
The Nano-Glo™ Dual-Luciferase Reporter (NanoDLR) Assay System is a homogeneous reagent system that allows you to sequentially detect the activities of firefly (Photinus pyralis) luciferase and NanoLuc luciferase (Nluc) from a single sample.
The Nano-Glo In-Gel Detection System directly detects the luminescence of NanoLuc fusion proteins in polyacrylamide gels after protein separation by PAGE under native or denaturing SDS conditions (native PAGE or SDS-PAGE).
The promoter-driven NanoLuc (Nluc) control vectors can be used to co-transfect with experimental firefly luciferase vectors when using the Nano-Glo™ Dual-Luciferase Reporter (NanoDLR) Assay System.
DNA-Dependent Protein Kinase Peptide Substrate is a highly specific substrate for DNA-PK, with the sequence Glu-Pro-Pro-Leu-Ser-Gln-Glu-Ala-Phe-Ala-Asp-Leu-Trp-Lys-Lys.
pHAb Amine Reactive Dye exhibits very low fluorescence at pH > 7 and much higher fluorescence as the pH of the solution becomes acidic. pHAb Dyes are designed specifically for antibody labeling.
The Proteasome-Glo™ Cell-Based Assays are homogeneous, luminescent assays that measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in a purified enzyme preparation.
Provides a biologically relevant and specific mechanism-of-action-based measure of ADCC without the complex workflow and variability inherent in primary cell-based assays. The bioassay also can be used to quantify effects of antibody glycosylation on Fc effector function.