Bioluminescence Assays and Reagents
Biotium Steady-Luc Firefly HTS Assay Kit (Lyophilized)
Firefly luciferase is a widely used reporter for studying gene function and regulation in mammalian cells or tissues as they lack endogenous luciferase activity. Light production from the reaction however, causes build-up of toxic adenyl-oxyluciferin at the enzyme surface that shortens half-life of light emission (flash-type) and makes batch-analysis or high-throughput screening (HTS) impossible. The Steady-Luc™ HTS assay is a proprietary formulation that extends the signal half-life to ~3h (steady-glow) by preventing adenyl-oxyluciferin build-up. The high-sensitivity, homogeneous, glow-type reporter gene assay allows high-throughput quantification of luciferase expression in mammalian cells in microplates. The lyophilized format allows room temperature shipping and -20°C storage. It provides excellent performance at an economical priced to compared to other brands.
Invivogen Quanti-Luc, Luciferase Reporter Gene Assay and Transfection System
Quanti-Luc is a luciferase reporter gene assay system from InvivoGen used to study gene expression. It contains a luciferase reporter gene under the control of an NF-κB-inducible ELAM-1 promoter that allows real-time monitoring of NF-κB activation. This kit includes a HEK-Blue Null1 cell line that stably expresses the Quanti-Luc reporter construct. When NF-κB is activated, the luciferase gene is expressed, allowing quantification of NF-κB activation by measuring luciferase activity with Quanti-Luc reagent. This assay provides a fast, sensitive system to monitor NF-κB activation and screen immunomodulatory compounds.
Cayman Chemical THUNDRTotal STAT5 TRFRET 9600
An MCT4 inhibitor (IC50 1.3 nM) selectively inhibits lactate influx in NCI H358 cells (IC50 6.1 nM) which highly express MCT4 and do not express MCT1 over lactate influx in K562 cells (IC50 >30 UM) which highly express MCT1 and do not express MCT4 inhibits the proliferation of NCI H358 cells (IC50 26 nM) reduces tumor growth in an NCI H358 mouse xenograft model at 100 mg/kg twice per day in combination with cediranib
Cayman Chemical THUNDRTotal STAT5 TRFRET 4800
An MCT4 inhibitor (IC50 1.3 nM) selectively inhibits lactate influx in NCI H358 cells (IC50 6.1 nM) which highly express MCT4 and do not express MCT1 over lactate influx in K562 cells (IC50 >30 UM) which highly express MCT1 and do not express MCT4 inhibits the proliferation of NCI H358 cells (IC50 26 nM) reduces tumor growth in an NCI H358 mouse xenograft model at 100 mg/kg twice per day in combination with cediranib
Revvity Health Sciences Inc twinlite Firefly and Renilla Luciferase Reporter Gene Assay System, 1,000 Assay Points
twinlite Firefly and Renilla Luciferase Reporter Gene Assay System, 1,000 Assay Points
Cayman Chemical Cy5 FIrefly LucIfRase mRNA 1mg
An amine-functionalized hydrophilic polymer a polysarcosine-labeled form of DSPE polysarcosines have been used as alternatives to PEGylation in LNPs and to reduce the immunogenicity of administered proteins in vivo
Cayman Chemical Firefly Luciferase mRNA Cap-
A Pim-3 kinase inhibitor (IC50 0.05 UM) selective for Pim-3 over Pim-1 and Pim-2 (IC50s 2.5 UM for both) inhibits the growth of DU145 and MIA PaCa-2 cells (IC50s 0.9 and 3.9 UM respectively) inhibits IL-6-induced STAT3 phosphorylation in DU145 cells at 10 UM
Cayman Chemical CyprIdIna LucIfRIntrIfluor
A natural non-caloric sweetener and hexose monosaccharide metabolized by gut bacteria in the colon of pigs
Cayman Chemical MyrIcetIn 3-galactosIde 10mg
A stimulator of soluble guanylate cyclase and an active metabolite of riociguat increases phosphorylation of the PKG substrate VASP in isolated rat aortic smooth muscle cells (EC50 353 nM) induces vasodilation in rat aortic rings (EC50 75 nM) decreases urine output and cardiac hypertrophy as well as improves survival in spontaneously hypertensive stroke-prone rats fed a high-salt high-fat diet at 3 mg/kg improves erectile responses to electrical stimulation of the cavernous nerve in a rat model of cavernous nerve crush injury at 0 03 and 0 3 mg/kg