Gel Filtration Media and Columns

Gel Filtration Media and Columns
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Filtered Search Results

Cytiva Gel Filtration Calibration Kits

Calibrate gel filtration columns simply, reliably. Cytiva Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards.
Content And Storage | 2° to 8°C |
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Product Type | Calibration Kit |
For Use With (Equipment) | High-resolution media and prepacked column |
Quantity | Each |
For Use With (Application) | Calibration of high-resolution gel filtration media and column such as Superdex™, Superose and Sephacryl |
Cytiva Superdex 30 PG size exclusion chromatography media prepacked into HiLoad 26/600 column

Superdex 30 prep grade (PG) size exclusion chromatography (gel filtration) media prepacked into a HiLoad 26/600 column is optimized for the separation of proteins and peptides, but can also be used with success to separate oligosaccharides.
Cytiva Sephadex™ G-25 Media
Use of Sephadex™ is well established as a gel filtration medium for desalting and buffer exchange in industrial applications. Cytiva Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step.
Height (Metric) | 30cm |
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Content And Storage | 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH |
Bed Volume | 4 to 6mL/g in Distilled Water |
Format | Solid Spherical Bead |
pH Range | 2 to 13 |
Product Type | Sephadex G-25 Media |
Diameter (Metric) Inner | 2.6cm |
For Use With (Application) | Desalting and Buffer exchange and removal free label in industrial applications |
Support Type | Cross-linked Dextran |
Height (English) | 11.81 in. |
Cytiva HiLoad™ Superdex™ 200 PG XK Column
Prepacked XK columns designed for high-resolution preparative gel filtration chromatography.
Content And Storage | 4 to 30°C, 20% Ethanol |
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Cytiva Sephadex™ G-50 and G-100 DNA Grade Agarose Gels

Used for purification of DNA from small molecules. Cytiva illustra™ Sephadex™ G-50 and G-100 DNA Grade Agarose Gels purify DNA by gel filtration.
Content And Storage | 4° to 30°C |
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Format | Beaded Gel |
pH Range | 2 to 10 |
Product Type | G-50 and G-100 DNA Grade Agarose Gels |
For Use With (Equipment) | Auto Seq G-50 column, ProbeQuant G-50 micro column, MicroSpin G-50 column and NICK column |
Sample Type | DNA |
Support Type | Cross-linked Dextran with Epichlorohydrin |
Cytiva Superdex™ 75 Prep Grade
Offers high resolution fractionation for separation, polishing and product formulation of recombinant proteins
Cytiva Sephadex™ G50 Superfine Media
Use as a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. Sephadex™ G50 Superfine Media's small bead size gives higher efficiency.
Particle Size | 25 to 75 μm |
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Height (Metric) | 60cm |
Content And Storage | 4°C to 30°C, 20% Ethanol |
pH Range | 3 to 11 |
Product Type | Sephacryl HR Column |
For Use With (Equipment) | Low to medium pressure AKTA™ systems |
Sample Type | Peptides and small proteins |
Quantity | Each |
Material | Polypropylene |
For Use With (Application) | Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix |
Support Type | Allyl Dextran and N, N'-methylenebisacrylamide |
Max. Pressure | 1.5 bar [0.15 MPa] (22 psi) |
Height (English) | 23.62 in. |
Height (Metric) | 10cm |
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Content And Storage | 4 to 30°C |
Bed Volume | 9 to 11mg/g |
pH Range | 2 to 10 |
Diameter (Metric) Inner | 5cm |
For Use With (Application) | For the separation of molecules Mr >30 000 from molecules Mr <1500 such as labeled protein or DNA from unconjugated dyesFor removeing small nucleotides from longer chain nucleic acids |
Max. Pressure | min 150 cm/h, bed height 10 cm, column 5 cm i.d. |
Particle Size | 34 to 208 μm in wet form, 20 to 80 μm in dry form |
Flow Rate | < Darcy´s Law |
Format | Solid Bead |
Product Type | Sephadex G50 Media |
Sample Type | DNA |
Support Type | Cross-linked Dextran |
Height (English) | 3.93 in. |
Cytiva IgG Sepharose™ 6 Fast Flow Medium

Use for rapid purification of protein A and protein A fusion conjugates. IgG Sepharose™ 6 Fast Flow Medium achieves the highest flowrates.
Cytiva HiTrap™ NHS-Activated HP Columns

Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.

Particle Size | 34 μm |
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Ligand Type | 10μM NHS groups/mL medium |
Height (Metric) | 2.5cm |
Content And Storage | 4° to 8°C |
pH Range | 3 to 12 |
Product Type | NHS-Activated HP Column |
For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
Material | Polypropylene |
For Use With (Application) | Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines |
Support Type | 6% Highly Cross-linked Agarose |
Max. Pressure | 42psi |
Height (English) | 0.98 in. |
Cytiva HiTrap™ Capto™ Core 400
Designed for intermediate purification and polishing of viruses and other large biomolecules