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Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.
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16 – 30 of 5,102 results
16
Promotions Available

Cytiva HisTrap™ FF Columns: 16 x 25mm
GSA_VA

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose
17

Cytiva GSTrap™ HP Columns

Use to provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins. Cytiva Life Sciences™ GSTrap™ HP Columns are prepacked with Glutathione Sepharose™ High Performance medium.

Particle Size 34 μm
Ligand Type Glutathione
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Description GSTrap HP columns are prepacked with Glutathione Sepharose High Performance resin and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
For Use With (Application) Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins
Support Type 4% Highly Cross-linked Agarose
18

Cytiva™ HiTrap™ Capto™ Core 700

The HiTrap™ Capto™ Core 700 is prepacked with Capto Core 700, a multimodal BioProcess resin for intermediate purification and polishing of viruses and other large biomolecules.

19
Promotions Available

Cytiva His GraviTrap™ Columns

Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.

20

Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents
GSA_VA

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

EDGE
Buffer Lysis Buffer
Shipping Condition Ambient
Form Liquid
Product Type Nuclear and Cytoplasmic Extraction Reagent
Reagent Type Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation
Product Line NE-PER™
21

Thermo Scientific™ Pierce™ Anti-DYKDDDDK Magnetic Agarose

Thermo Scientific™ Pierce™ Anti-DYKDDDK Magnetic Agarose Beads provide a fast, convenient method for purification of DYKDDDK-tagged recombinant proteins

EDGE
dimMatrix Magnetic Agarose
22

Thermo Scientific™ Mitochondria Isolation Kit for Cultured Cells
GSA_VA

Isolate intact mitochondria from cultured mammalian cell samples in about 40 minutes with this versatile, benchtop microcentrifuge-based fractionation kit.

EDGE
23

Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells
GSA_VA

Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.

EDGE
24

Thermo Scientific™ Mitochondria Isolation Kit for Tissue
GSA_VA

Isolate intact mitochondria from soft and hard tissue samples in about an hour with this versatile, benchtop microcentrifuge-based fractionation kit.

EDGE
25

Thermo Scientific™ Pierce™ Agarose ChIP Kit
GSA_VA

Chromatin immunoprecipitation (chromatin IP, ChIP) assay kit containing all reagents, agarose affinity beads, and a simple, fast, reliable protocol.

EDGE
26

Thermo Scientific™ Pierce™ Magnetic RNA-Protein Pull-Down Kit
GSA_VA

Utilize a streamlined, robust method to enrich protein-RNA interactions using end-labeled RNA as the bait with Thermo Scientific™ Pierce Magnetic RNA-Protein Pull-Down Kit.

EDGE
27

Thermo Scientific™ HisPur™ Ni-NTA Chromatography Cartridges, 1 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

EDGE
Stationary Phase Divalent Nickel, Metal Chelate
Type Chromatography Cartridge
Target His-Tagged Protein
Content And Storage Store between 4°C and 8°C. Do not freeze.
Column Type Affinity, Agarose Resin
Product Line HisPur™
28

Thermo Scientific™ Pierce™ GST Protein Interaction Pull-Down Kit

Easily capture and affinity purify binding partners of GST-tagged fusion proteins with these glutathione (GSH) agarose beads and complete reagent set.

EDGE
29

Thermo Scientific™ Detergent Removal Spin Columns & Plates

Rapidly eliminate high concentrations of detergents with minimal sample loss with detergent removal resins. Thermo Scientific HiPPR (High Protein and Peptide Recovery) resin is recommended for protein concentrations up to 100 μg/mL, while Thermo Scientific Pierce resin is suitable for concentrations over 100 μg/mL.

EDGE
Stationary Phase Proprietary Ligand
Column Type Proprietary Resin, Affinity
Purification Target Detergent Removal
30

Thermo Scientific™ Tetraethylene Glycol Monooctyl Ether (C8E4), LC/MS grade

Tetraethylene Glycol Monooctyl Ether (C8E4) is a non-ionic surfactant that possesses unique properties making it suitable for solubilization of sensitive biomolecules without significant denaturation or disruption of their structure and function.

EDGE