Restriction Enzymes
New England Biolabs, Inc. BstAPI – 1000 units
A restriction endonuclease that recognizes the sequence GCAN_NNN^NTGC.
New England Biolabs, Inc. BsaI-HF®v2 – 1000 units
BsaI-HFv2 is a Type IIS restriction enzyme that has been optimized for Golden Gate Assembly and in protocols requiring DNA cutting by BsaI. Works well for any protocol requiring DNA cutting by BsaI. Recommended enzyme for any purpose requiring digestion at the recognition sequence ...5-GGTCTC(N1)/(N5)-3...
- Engineered for improved performance
- Used in Golden Gate Assembly; maintains high activity levels in T4 DNA Ligase Buffer
- Type IIS REs recognize asymmetric DNA sequences; cleave outside their recognition sequence
- 100% activity in rCutSmart Buffer
- Time-Saver qualified for digestion in 5-15 minutes
New England Biolabs, Inc. Nb.BssSI – 1000 units
A restriction endonuclease that recognizes the sequence CACGAG.
New England Biolabs, Inc. EcoRI-HF – 50000 units
A high fidelity restriction endonuclease that recognizes the sequence G^AATT_C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. BsaI-HF®v2 – 5000 units
BsaI-HFv2 is a Type IIS restriction enzyme that has been optimized for Golden Gate Assembly and in protocols requiring DNA cutting by BsaI. Works well for any protocol requiring DNA cutting by BsaI. Recommended enzyme for any purpose requiring digestion at the recognition sequence ...5-GGTCTC(N1)/(N5)-3...
- Engineered for improved performance
- Used in Golden Gate Assembly; maintains high activity levels in T4 DNA Ligase Buffer
- Type IIS REs recognize asymmetric DNA sequences; cleave outside their recognition sequence
- 100% activity in rCutSmart Buffer
- Time-Saver qualified for digestion in 5-15 minutes
New England Biolabs, Inc. NgoMIV – 1000 units
A restriction endonuclease that recognizes the sequence G^CCGG_C.
New England Biolabs, Inc. AvrII – 100 units
A restriction endonuclease that recognizes the sequence C^CTAG_G.
New England Biolabs, Inc. EcoRV – 4000 units
A restriction endonuclease that recognizes the sequence GAT_^ATC.
New England Biolabs, Inc. XhoI – 25000 units
A restriction endonuclease that recognizes the sequence C^TCGA_G.
New England Biolabs, Inc. AflII – 2000 units
A restriction endonuclease that recognizes the sequence C^TTAA_G.
New England Biolabs, Inc. PmeI – 500 units
A restriction endonuclease that recognizes the sequence GTTT_^AAAC.
New England Biolabs, Inc. PshAI – 1000 units
A restriction endonuclease that recognizes the sequence GACNN_^NNGTC.
New England Biolabs, Inc. MseI – 500 units
A restriction endonuclease that recognizes the sequence T^TA_A.
New England Biolabs, Inc. BsiHKAI – 1000 units
A restriction endonuclease that recognizes the sequence G_WGCW^C.
New England Biolabs, Inc. BsrFI-v2 – 1000 units
A restriction endonuclease that recognizes the sequence R^CCGG_Y.