Restriction Enzymes
New England Biolabs, Inc. Exonuclease V (RecBCD) – 5000 units
Exonuclease V, a RecBCD complex from E. coli has several different enzyme activities, including an ATP-dependent single-stranded DNA endonuclease activity, ss- and ds- DNA exonuclease activity. The hydrolysis in each case is bi-directional (from both the 3' and 5' ends) and processive, producing oligonucleotides. All Exonuclease V activities have divalent cation requirements. Mg2+ is required for the exonuclease activity, while Ca2+ inhibits the exonuclease activity and allows double-stranded DNA unwinding (helicase activity) without hydrolysis.
Agilent Technologies DPN I RESTRICTION ENZYME
Dpn I Restriction Enzyme, is negative for nonspecific nucleases. It is used when cloning into multiple cloning site of pBluescript II phagemid vector. It includes an optimal (10X) buffer and/or a 10X concentrate of Universal buffer.
New England Biolabs, Inc. BtgZI – 500 units
A restriction endonuclease that recognizes the sequence GCGATGNNNNNNNNNN^NNNN_.
New England Biolabs, Inc. BspEI – 5000 units
A restriction endonuclease that recognizes the sequence T^CCGG_A.
New England Biolabs, Inc. I-SceI – 500 units
A restriction endonuclease that recognizes the sequence TAGGG_ATAA^CAGGGTAAT. I-SceI is an intron-encoded endonuclease. The intron encoding I-SceI is present in the mitochondria of Saccharomyces cerevisiae.
New England Biolabs, Inc. PspGI – 1000 units
A restriction endonuclease that recognizes the sequence ^CCWGG_.
New England Biolabs, Inc. HpyCH4V – 100 units
A restriction endonuclease that recognizes the sequence TG_^CA.
New England Biolabs, Inc. Hpy99I – 100 units
A restriction endonuclease that recognizes the sequence _CGWCG^.
New England Biolabs, Inc. SapI – 1250 units
A restriction endonuclease that recognizes the sequence GCTCTTCN^NNN_.
New England Biolabs, Inc. MlyI – 1000 units
A restriction endonuclease that recognizes the sequence GAGTCNNNNN_^.
New England Biolabs, Inc. BfaI – 2500 units
A restriction endonuclease that recognizes the sequence C^TA_G.
New England Biolabs, Inc. HpyCH4III – 1250 units
A restriction endonuclease that recognizes the sequence AC_N^GT.
New England Biolabs, Inc. HpyCH4V – 500 units
A restriction endonuclease that recognizes the sequence TG_^CA.
New England Biolabs, Inc. BslI – 1000 units
A restriction endonuclease that recognizes the sequence CCNN_NNN^NNGG.
New England Biolabs, Inc. MlyI – 5000 units
A restriction endonuclease that recognizes the sequence GAGTCNNNNN_^.