Substrates
![Research Products International Corp MUG [4-Methylumbelliferyl-B-D-glucuronide Trihydrate], 100 Milligrams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M65900-0.1.JPG-250.jpg)
Research Products International Corp MUG [4-Methylumbelliferyl-B-D-glucuronide Trihydrate], 100 Milligrams
Fluorescent substrate for β-D-Glucuronidase (GUS) encoded by the gusA gene isolated from E. coli. Fluorescence assay allows quantitation of GUS activity in protein extracts at peak excitation of 365 nm (UV) and a peak emission of 455 nm (blue).
![Research Products International Corp Red-GAL [6-Chloro-3-indolyl-B-D-galactopyranoside], 1 Gram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C40040-1.0.JPG-250.jpg)
Research Products International Corp Red-GAL [6-Chloro-3-indolyl-B-D-galactopyranoside], 1 Gram
An alternative chromogenic substrate for β-D-galactosidase. Red-GAL is used in conjuction with IPTG for detection of β-galactosidase activity in bacterial colonies in a colorimetric assay to detect recombinants from non-recombinants.
Biotium NucView 488 Caspase-3 Substrate, 1mM in 1X PBS
NucView Caspase-3 Substrates are novel fluorogenic substrates for real-time or endpoint detection of apoptosis in live cells. The non-toxic, no-wash substrates do not inhibit caspase-3 unlike other caspase substrates or FLICA assays permitting multiday analysis. They consist of a high-affinity DNA dye linked to a DEVD caspase-3 substrate moiety. Initially non-fluorescent, the cell permeable substrate is cleaved by caspase-3 in apoptotic cells and emits fluorescence, for detection of caspase-3 activity and apopototic nuclear morphology. Suitable for flow cytometry, fluorescence microscopy, or live cell imaging systems, staining is compatible with fixation and permeabilization for immunostaining. NucView 488 is validated in over 100 cell types and 200 publications. Available with blue (NucView 405), green (NucView 488), or red (NucView 530) fluorescence, as PBS solutions for DMSO-sensitive cells, and as stand-alone assays or in kits with other apoptosis or necrosis probes.
Medchemexpress LLC N-acetyl-L-tryptophyl-L-leucyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-L-alaninamide | 1104011-59-7 | 99.8% | 587.67 | C32H37N5O6 | 5MG
Ac-WLA-AMC is a fluorogenic peptide substrate selective for the chymotrypsin-like (β5) subunit of the 20S constitutive proteasome. Upon enzymatic cleavage the 7-amino-4-methylcoumarin (AMC) fluorophore is released, enabling sensitive fluorometric measurement of proteasome β5 activity in biochemical assays. Supplied as a high-purity solid for research use.
Medchemexpress LLC HY-12591A 50mg Medchemexpress, D-Luciferin D-(-)-Luciferin;Firefly luciferin CAS:2591-17-5 Purity:98%
Medchemexpress,HY-12591A 50mg D-Luciferin D-(-)-Luciferin;Firefly luciferin CAS:2591-17-5 Formula:C11H8N2O3S2 D-luciferin is the natural substrate of luciferases that catalyze the production of light in bioluminescent insects. Purity:98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
Seracare Life Sciences Inc SureBlue™ TMB 1-Component Microwell Peroxidase Substrate
SureBlue TMB Microwell Substrate develops a deep blue color in the presence of peroxidase-labeled conjugates measurable at 650 nm and turns yellow when stopped by acidification and read at 450 nm. Recommended for ELISA and blotting applications with addition of membrane enhancer. Single component, ready to use format of 3,3', 5, 5' - Tetramethylbenzidine exibits exceptional stability and lot-to-lot consistency. No reagent preparation required, very convenient.
Requires storage 2-8 degrees Celsius.
Ubpbio K48-linked di-Ubiquitin (human), enzymatically synthesized
Ub chains are formed by conjugating the C-terminal glycine residue of Ub onto any of seven internal lysine residues or the amino group of the previous Ub. Ub chains are classified by the lysine residue used to link Ubs; different Ub chain topologies can result in different signals. For instance, Ub chains linked through lysine 6, 11, 27, 29, 33 and 48 are capable of targeting proteins for proteasomal degradation; in contrast, Ub chains linked through lysine 63 or the N-terminal amino group (linear Ub chains) often play important nonproteolytic functions including regulation of kinase activation and protein translation. All Ub chain products are produced by using of human wild type Ub reacting with specific E2s.
Medchemexpress LLC Z-DEVD-AFC | 1135416-11-3 | 98.1% | 821.71 | 10 MG
Z-DEVD-AFC is a cell-permeant substrate for caspase-3, which causes a shift in fluorescence upon cleavage of the AFC fluorophore.
- Can be used to detect caspase-3-like enzyme activity.
- For research use only.
Medchemexpress LLC MBP-MAPK-Substrate | 138028-00-9 | 99.6% | 967.09 | 5 MG
MBP MAPK Substrate is utilized as an exogenous substrate for MAPK. It is intended for research use only, and not for sale to patients.
- Used as an exogenous substrate for MAPK
- Appearance: solid
- Color: white to off-white
- Sequence: Ala-Pro-Arg-Thr-Pro-Gly-Gly-Arg-R
- Sequence shortening: APRTPGGRR
- Target: p38 MAPK
- Pathway: MAPK/ERK pathway
Medchemexpress LLC Z-DEVD-AFC | 1135416-11-3 | 98.1% | 821.71 | 5 MG
Z-DEVD-AFC is a cell-permeant substrate for caspase-3. It causes a shift in fluorescence upon cleavage of the AFC fluorophore, allowing for detection of caspase-3-like enzyme activity. This product is for research use only.
- Cell-permeant substrate for caspase-3
- Fluorescence shift upon AFC fluorophore cleavage
- Detects caspase-3-like enzyme activity
- White to off-white solid
- Emission (Em): 500
- Excitation (Ex): 380
Medchemexpress LLC Z-leu-leu-glu-AMC | 348086-66-8 | MFCD01074989 | 99.9% | 664.75 | C35H44N4O9 | 10MM 1ML
Z-Leu-Leu-Glu-AMC is a fluorogenic tripeptide substrate used to measure postacidic-like (peptidylglutamyl-peptide-hydrolyzing) activity of the 20S proteasome. Proteolytic cleavage releases 7-amino-4-methylcoumarin (AMC), producing a fluorescent signal suitable for proteasome activity assays, kinetic studies, and inhibitor screening. The product is available as a ready-to-use solution in DMSO and in multiple solid pack sizes for custom preparations.
- Fluorogenic substrate that releases AMC upon cleavage.
- Sensitive assay readout suitable for kinetic measurements.
- Provided as ready-to-use 10 mM solution in DMSO or as solids for custom preparation.
- High purity confirmed by LCMS.
- Compatible with 20S and 26S proteasome activity assays.
Cayman Chemical Abl Substrate Peptide triflu
A peptide substrate for Abl; selectively phosphorylated by Abl over c-Src kinase at 20 µM; has been used to quantify Abl kinase activity in vitro
Vector Laboratories DAB Enhancing Solution SK-4103-400
DAB Enhancing Solution, 30 ml, intensifies the DAB reaction product in stained sections. A ten-second exposure to this solution darkens the reaction product.
Bioworld ABTS, Diammonium Salt, 1 g
ABTS, Diammonium Salt Also known as 2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid). Technical Information:Ultra Pure GradePurity: >98.0%Em (225nm, H2O): >48.150Solubility (H2O): 5%Store: Frozen