This kit can be used for rapid extraction of RNA from various trace samples, and does not rely on toxic reagents such as phenol and chloroform. The unique lysis buffer rapidly lyses cells from trace samples and inactivates cell RNA enzymes. The lysate mixture passes through a gDNA Remove Column, where gDNA is removed and RNA is filtered through. After adjusting the binding conditions by ethanol, RNA is selectively bound to the silicon membrane of the adsorption column in the state of high disordered salt. In a series of rapid washing-centrifugation steps, the purified RNA is eluted from the silicon membrane with low salt RNase-free H2O. The extracted RNA can be directly used in RT-PCR, qPCR and RNA library constructing experiments.