Biotinylated Human HGF R Protein, His,Avitag™ is expressed from human 293 cells (HEK293). It contains amino acids Glu 25 – Asp 741 (Accession # P08581-1) and carries a C-terminal polyhistidine tag and an Avi tag (Avitag™). Biotinylation is performed using Avitag™ technology. HGF R, or c-MET, is a receptor tyrosine kinase involved in cellular survival, embryogenesis, and migration. The protein has a calculated molecular weight of 84.4 kDa, migrating at 60–70 kDa under reducing conditions.
- Expressed from human 293 cells (HEK293)
- Features C-terminal polyhistidine and Avi tags (Avitag™)
- Biotinylated via enzymatic labeling of a single lysine in the Avitag™
- Purity is >90% by SDS-PAGE
- Exhibits bioactivity by binding Human HGF, Fc Tag and Biotinylated Human HGF R Protein