The term chromatography describes a variety of techniques that are used to separate a substance or mixture into its components. Many products are used during different stages of chromatography workflows, including solvents, standards, columns, vials and closures, and chromatography paper.
Chromatography can be preparative or analytical. It can be used to prepare a substance for analysis or used to measure the amount or proportion of specific components in the substance.
The choice of chromatography method depends on the specific application and substance, as well as the polarity of the separation media and solvent. Commonly used chromatography methods include:
- Liquid chromatography
- High performance liquid chromatography (HPLC): One of the most frequently used research and analytical techniques; great for small molecule analysis
- Flash chromatography: Ideal for separating large sample volumes
- Fast protein liquid chromatography (FPLC): Uses ion exchange to separate large biomolecules
- Thin layer chromatography: Silica coated glass or alumina plates are dotted with a substance; one end of the plate is immersed in solvent, which carries and separates the components as it travels
- Gas chromatography
- Samples are heated to a gaseous state
- Well-suited for petrochemical, environmental, and industrial chemical research and testing
Liquid chromatography columns for HPLC, UHPLC, FPLC, and LC-MS are available for small and large molecule (biomolecule) analysis. They come in reversed phase (C18, C8, RP-amide polar-embedded, phenyl, diol, cyano), normal phase, ionic, size exclusion, and preparatory column varieties. For optimal LC system performance, consider high-quality solvents and buffers, LC calibration standards, autosampler vials, solid phase extraction cartridges, and other LC accessories.
GC and GC/MS capillary gas chromatography columns serve multiple industries, including environmental, petroleum, chemical, food and beverage, and pharmaceutical. Packed GC columns and components are also availble. To maximize performance, choose compatible GC accessories, including GC inlet liners, septa, syringes, vials, calibration standards, extraction solvents, and gas management products (including gas generators and pressure regulators).
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