Connexin 43 Recombinant Rabbit Monoclonal Antibody (PSH0-32), Invitrogen™

Rabbit Recombinant Monoclonal Antibody

Supplier:  Thermo Scientific MA549139

Catalog No. PIMA549139

Description

Sequence Similarities: 95% Mouse/Rat Tissue Specificity: Expressed in the heart and fetal cochlea. Positive Control: A549 cell lysate, PC-12 cell lysate, mouse heart lysate, human heart tissue, human testis tissue. Subcellular location: Plasma membrane. Endoplasmic reticulum. Cell junction, gap junction.

Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.

Specifications

• Antigen: Connexin 43
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.1% BSA, 40% glycerol and 0.05% sodium azide; pH 7.4
• Gene Accession No.: P08050, P17302, P23242
• Gene Symbols: GJA1
• Immunogen: Recombinant protein within human connexin 43 aa 219-382.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Form: Liquid

• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Clone: PSH0-32
• Conjugate: Unconjugated
• Gene: GJA1
• Gene Alias: alpha 1 connexin; AU042049; AVSD3; AW546267; CMDR; Cnx43; connexin 43; connexin43; connexin-43; cx43; Cx-43; cx43 protein; cx43a1; Cx43alpha1; CXA1; Cxn-43; DFNB38; EKVP; etID309742.9; gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction membrane channel protein; gap junction membrane channel protein alpha 1; gap junction protein; gap junction protein alpha 1; gap junction protein connexin 43; gap junction protein, alpha 1; gap junction protein, alpha 1, 43 kD (connexin 43); gap junction protein, alpha 1, 43kDa; gap junction protein, alpha 1, 43kDa (connexin 43); gja1; Gja-1; GJAL; HLHS1; HSS; Npm1; ODD; ODDD; ODOD; PPKCA; SDTY3; shf; Short fin protein; sof; stopsel; stp; Syndactyly type III; vascular smooth muscle connexin43; Vascular smooth muscle connexin-43; zfCx43.3
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 14609, 24392, 2697
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov