Invitrogen™ Human Periostin ELISA Kit

ELISA

Catalog Number	Mfr. No.	Quantity
EHPOSTN	Invitrogen™ EHPOSTN	96 Tests
EHPOSTNX5	Invitrogen™ EHPOSTNX5	5 x 96 Tests
EHPOSTNX10	Invitrogen™ EHPOSTNX10	10 x 96 Tests

Description

The Human Periostin ELISA quantitates Hu Periostin in human serum,plasma,or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PN. Principle of the method The Human Periostin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples,standards,or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody,a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

Periostin (PN), also designated osteoblast-specific factor 2 (OSF-2), is a disulfide linked protein originally isolated as a osteoblast-specific factor. Periostin is a secreted protein that binds heparin and functions as a ligand for alpha(V)beta(3) and alpha(V)beta(5) integrins. In preosteoblasts, Periostin acts as a cell adhesion molecule and plays a role in osteoblast recruitment, spreading and attachment. Periostin is mainly detected in lower gastrointestinal tract, aorta, stomach, placenta, uterus and breast tissues but is up-regulated in epithelial ovarian tumors and overexpressed in breast cancer. Expression of Periostin is increased by bone morphogenetic protein (BMP2) and transforming growth factor beta 1(TGF beta 1). Periostin contains a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain, which shows homology with the insect protein fasciclin, and a C-terminal domain.

Specifications

• Accession Number: Q15063
• Assay Sensitivity: 80 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: OSF-2,OSF2,PDLPOSTN,PN
• Intraassay CV: <10
• Label or Dye: HRP
• Research Discipline: Extracellular Matrix & Cell Adhesion
• Storage Requirements: 2°C to 8°C
• Target: Periostin
• Total Assay Time: 4 hrs 45 mins

• Assay Range: 0.082 to 60 ng/mL
• Calibration Range: 80 pg/mL to 60 ng/mL
• Conjugate: HRP
• Protein Family: Cell Structure & Organelle Proteins
• Specificity: This ELISA pair antibody detects human Periostin. Other species not determined.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 10631
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Synonym: RP11-412K4.1, OSF-2, OSF2, PDLPOSTN, PN, periostin
• Test Time: 1 hrs 20 mins