Invitrogen™ Platinum™ SuperFi™ DNA Polymerase

Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.

Supplier:  Invitrogen™ 12351250

Catalog No. 12-351-250

Description

Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. Featuring >300X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.

Benefits of Platinum SuperFi DNA Polymerase

• Exceptional >300X Taq fidelity
• High specificity and increased yields with Platinum hot-start technology
• Robust amplification of difficult targets including those of sub-optimal purity or high GC content
• Room temperature reaction setup and 24 hour bench top stability of pre-assembled reactions
• Amplification of samples with suboptimal purity

Applications

• High-fidelity PCR
• Cloning and sub-cloning
• Site-directed mutagenesis
• Amplification of GC-rich templates
• Template generation for sequencing
• High-throughput PCR
• Long PCR

Notes

• Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).

Specifications

• Content And Storage: 5 boxes of Platinum SuperFi Master Mix, each containing:
  • Platinum SuperFi DNA Polymerase (250 μL at 2 U/μL)
  • 5X SuperFi Buffer (6 x 1.25 mL)
  • 5X SuperFi GC Enhancer (4 x 1.25 mL)
  
  Store at –5°C to –30°C.
• Polymerase: Platinum SuperFi DNA Polymerase
• Quantity: 5 x 500 units
• For Use With (Application): Hot-start PCR, High-fidelity PCR
• Hot Start: Built-In Hot Start
• Overhang: Blunt
• Size (Final Product): 20 kb or less

• GC-Rich PCR Performance: High
• Reaction Speed: Fast
• Shipping Condition: Wet or Dry Ice
• Fidelity (vs. Taq): 300X
• No. of Reactions: 5 x 500 Reactions
• Reaction Format: Separate Components

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.