UFD1L Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA5120864

Catalog No. PIPA5120864

Description

Positive test controls include: MCF7, A-431, Mouse heart. The target is usually found in the following locations: Cytoplasm, Nucleus, cytosol. Immunogen sequence: MFSFNMFDHP IPRVFQNRFS TQYRCFSVSM LAGPNDRSDV EKGGKIIMPP SALDQLSRLN ITYPMLFKLT NKNSDRMTHC GVLEFVADEG ICYLPHWMMQ NLLLEEGGLV QVESVNLQVA TYSKFQPQSP DFLDITNPKA VLENALRNFA CLTTGDVIAI NYNEKIYELR VMETKPDKAV SIIECDMNVD FDAPLGYKEP ERQVQHEEST EGEADHSGYA GELGFRAFSG SGNRLDGKKK GVEPSPSPIK PGDIKRGIPN YEFKLGKITF IRNSRPLVKK VEEDEAGGRF VAFSGEGQSL RKKGRKP

Ubiquitin-mediated proteolysis requires the transfer of ubiquitin (Ub) to lysine groups on selected cellular proteins, which then potentiates the proteolytic degradation of these protein conjugates by the 26S proteasome. Ub-fusions are cleaved by Ub-specific processing proteases (UBps) or alternatively by the Ub-fusion degradation (UFD) pathway. The UBP pathway targets the C-terminal glycine residue on Ub that is involved in the formation of Ub-conjugates, while UFD proteins preferentially cleave Ub-conjugated proteins that contain an amino acid substitution at this glycine residue. The UFD1 protein was originally characterized in the yeast S. cerevisiae and subsequently, the human homolog UFD1 or UFD1L was identified. In vitro, UFD1 attenuates the degradation of Ub-fusions, which have a proline or valine residue substituted at the Gly76 moiety, by the selective multiubiquitination of the Ub chain of the Ub-conjugate. Mutations within the UFD1 gene are implicated in the development of CATCH22 syndrome, which is characterized by cardiac defects, cleft palate and hypocalcemia, suggesting that this proteolytic pathway may be involved in the progression of these developmental defects.

Specifications

• Antigen: UFD1L
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: UFD1
• Gene Alias: RGD:619822}; UB fusion protein 1; UB fusion protein 1 {ECO:0000250; ubiquitin fusion degradation 1 like; ubiquitin fusion degradation 1 like (yeast); Ubiquitin fusion degradation protein 1; Ubiquitin fusion degradation protein 1 homolog; ubiquitin fusion degradation protein 1 homolog {ECO:0000250; Ubiquitin recognition factor in ER-associated degradation protein 1; UFD1; UFD1L; ufd1l {ECO:0000312; UniProtKB:Q92890}
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 22230, 7353
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunocytochemistry, Western Blot
• Concentration: 1.28 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: P70362, Q92890
• Gene Symbols: UFD1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human UFD1L (NP_005650.2).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG