VGLL2 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA575941

Catalog No. PIPA575941

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

VGLL2 is the master regulator in the wing development in Drosophila, which acts through a complex by interacting with scalloped, a homolog of vertebrate TEF proteins. Northern and dot blot analyses of human adult tissues detected VGLL2 expression in skeletal muscle. In situ hybridization revealed VGLL2 expression in differentiating somites and branchial arches during mouse embryogenesis. VITO1 is the clone of VGLL2. It contains a scalloped interaction domain (SID). VITO1 is more highly expressed in differentiated myotubes from the mouse C2C12 myogenic cell line than in undifferentiated cells. During differentiation in mouse C2C12 cell line, VGLL2 mRNA levels increase and VGLL2 protein translocates from the cytoplasm to the nucleus. Interference with VITO1 expression by small interfering RNA attenuated differentiation in C2C12 muscle cells. Therefore, this protein is an important component of the myogenic program.

Specifications

• Antigen: VGLL2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: VGLL2
• Gene Alias: C130057C21Rik; Protein VITO1; RP1-179P9.2; Transcription cofactor vestigial-like protein 2; Vestigial and Tondu related protein 1; vestigial like 2; vestigial like 2 homolog; vestigial like 2 homolog (Drosophila); vestigial like family member 2; vestigial-like 2; vestigial-like family member 2; Vgl2; vgl-2; Vgll2; Vito1; VITO-1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 215031, 245806, 309772
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q8BGW8, Q8N8G2
• Gene Symbols: VGLL2
• Immunogen: Synthetic peptide corresponding to amino acids 34-79 of Human VGLL2.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG