Recirculating Chillers
Sigma Aldrich Fine Chemicals Biosciences GSTrapTM High Performance
GSTrap(TM) HP columns are prepacked with Glutathione Sepharose(R) High Performance medium and provide high-reSOLUTIONon one-step purifications of glutathione S-Transferase (GST) tagged proteins.
Sigma Aldrich Fine Chemicals Biosciences TempliPhiTM 100 Amplificat
illustra(TM) TempliPhi(TM) DNA Amplification Kits use isothermal rolling circle amplification (RCA) to efficiently prepare DNA sequencing templates.TempliPhi(TM) Kits use a unique process to efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi(TM) uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 C enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi(TM) protocol requires less than 20 min of hands-on time to amplify 96 samples from bacterial colonies.Two different types of TempliPhi(TM) DNA Amplification Kits are available to meet different throughput requirements. illustra(TM) TempliPhi(TM) DNA Amplification Kit for 2000 reactions contains premixed components and generates templates after overnight incubation. The 100- an
Sigma Aldrich Fine Chemicals Biosciences Glass beads unwashed 425 61KG
Glass beads unwashed 425 61KG
Sigma Aldrich Fine Chemicals Biosciences Nitroreductase from Escher1MG
Nitroreductase is a flavoprotein and is encoded by the NfsB gene. It comprises a dimer with 217 amino acids and active site in each subunit. The structure has fMN and the substrate bound to the enzyme.
Sigma Aldrich Fine Chemicals Biosciences CelLyticTM NuCLEARTM Extra
The procedure for the nuclear protein extraction method is to allow cells to swell with hypotonic buffer. The cells are then disrupted the cytoplasmic fraction is removed and the nuclear proteins are released from the nuclei by a high salt buffer.
Sigma Aldrich Fine Chemicals Biosciences KiCqStartR SYBRR Green qPC
KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated ready-to-use reaction cocktail that contains all components except primers and template for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer stabilizers and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency sensitivity specificity and robust fluorescent signal using fast or conventional cycling protocols with SYBR Green qPCR.Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step. The optimized formulation includes SYBR Green I dye an antibody-mediated Hot-Start Taq DNA polymerase dNPTs MgCl2 and proprietary buffers and stabilizers.
Sigma Aldrich Fine Chemicals Biosciences EppendorfR ReferenceR 2 G1EA
Extraordinary precision and accuracy - EppendorfA´s premium pipetteBesides improved ergonomics the design focus lays on providing the most accurate results possible optimum user safety reliability and robustness for a long service life. The innovative single button operation is not only fast and easy but it also allows active aerosol1) reduction and thus protects the user the sample and the pipette. The sturdy handle manufactured with stainless steel protects the pipette from impact and in combination with the spring loaded bumpers the Reference 2 is likely to stay within calibration even if it takes a fall. These high levels of security make the Reference 2 ideal for use with precious liquids or in any application requiring extremely high levels of accuracy. When there can be no compromise on quality and reproducibility Eppendorfs Reference 2 is an ideal instrument for these liquid handling applications.Single-Button OperationLower Operating forces for aspiration/dispensing
Sigma Aldrich Fine Chemicals Biosciences Alkyltrimethylammonium bro500G
Alkyltrimethylammonium bromide is a surfactant and can be used to study the forces and stability of foam films produced from soluble cationic surfactants.
Sigma Aldrich Fine Chemicals Biosciences Mf MilliporeR Membrane fil
Biologically inert mixtures of cellulose acetate and cellulose nitrate have made Mf-Millipore membrane filters one of the most widely used membranes in analytical and research applications. Mf-Millipore filters without Triton surfactant contain minimum amounts of wetting agent and have a lower water extractable content than standard Mf-Millipore filters.features Benefits - Versatile filter for biological and environmental monitoring applications - Available in a range of pore sizes colored black or white with or without a gridded surface - Compatible with ethylene oxide gamma irradiation and autoclave sterilization methods
Sigma Aldrich Fine Chemicals Biosciences Claramine trifluoroacetate25MG
Claramine is an easy and quick to synthesize polyaminosteroid derivative. It is a trodusquemine-related compound and shares with it a spermino group which is responsible for its inhibitory action towards protein tyrosine phosphatase 1B (PTP1B).
Sigma Aldrich Fine Chemicals Biosciences Riboflavin 5-monophosphate5MG
Riboflavin 5AE-monophosphate (fMN) is highly soluble in water. flavins are considered as blue-light absorbing dyes.
Sigma Aldrich Fine Chemicals Biosciences Pyoverdines from Pseudomon1MG
Pyoverdines from Pseudomon1MG
Sigma Aldrich Fine Chemicals Biosciences PercollR Cytiva 17 0891 01
Percoll(R) is a well-referenced media for density gradient centrifugation of cells viruses and subcellular particles. Percoll(R) is composed of colloidal silica coated with polyvinylpyrrolidone (PVP).Percoll(R) is a low viscosity density gradient medium for preparation of cells subcellular particles and larger viruses. The low viscosity of the medium enables cell preparation on preformed gradients in only a few minutes using low centrifugal forces (200 to 1000 x g). The medium is available in easy-to-open resealable 250 mL and 1 l bottles.Percoll(R) consists of silica particles (15 to 30 nm diameter) coated with non-dialyzable polyvinylpyrrolidone (PVP). free PVP is present at only 1% to 2%. Percoll(R) is non-toxic almost chemically inert and does not adhere to membranes. Percoll(R) gradients can be formed within the density range of 1.0 to 1.3 g/mL and are iso-osmotic throughout.Percoll(R) can be stored unopened at room temperature for five years. At -20 C it can only be store