Enzo Life Sciences’ TOTAL-NUCLEAR-ID Green/Red Nucleolar/Nuclear Detection Kit contains two proprietary dyes suitable for simultaneous live-cell staining of nucleoli and nuclei. The dyes allow examination of nucleolar dynamic changes in intracellular distribution, trafficking and localization arising from biological processes such as the cell cycle and ribosome biogenesis. The kit is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms. This kit is specifically designed for visualizing nucleoli and nuclei in living cells. A control nucleolus perturbation agent, actinomycin D, is provided for monitoring changes in nucleolar dynamics. Applications: Fluorescence microscopy, Fluorescent detection .