Cell Based Assays
Promega ADP-Glo™ Kinase Assay + SLK Kinase Enzyme System
Recombinant full-length human SLK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. SLK or LOSK is a Ste20-like protein kinase that associates with microtubule and centrosome.
Promega p70S6Kb Kinase Enzyme System, 1mg
Recombinant full-length human p70S6Kb was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p70S6Kb is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases and is activated by mitogenic stimuli.
Promega MELK Kinase Enzyme System, 10microg
Recombinant human MELK (1-340) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MELK or maternal embryonic leucine zipper kinase is a member of the Snf1/AMPK kinase family.
Promega FGFR3 (K650E) Kinase Enzyme System, 1mg
Recombinant human FGFR3 (K650E) (397-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Fibroblast growth factor receptor 3 is part of a family of fibroblast growth factor receptors that share similar structure and function
Promega CDK3/CyclinE1 Kinase Enzyme System, 10microg
Recombinant full-length human CDK3 and Cyclin E1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK3/CyclinE1 is a member of the cyclin-dependent protein kinase family.
Promega MAPKAPK5 Kinase Enzyme System, 10microg
Recombinant full-length human MAPKAPK5 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MAPKAPK5 is a member of the serine/threonine kinase family that responds to cellular stress and proinflammatory cytokines.
Promega PKC theta Kinase Enzyme System, 1mg
Recombinant full-length human PKC theta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein Kinase C theta (PKC theta) is an important component in the intracellular signaling cascade.
Promega ADP-Glo™ Kinase Assay + EGFR (L861Q) Kinase Enzyme System
Recombinant human EGFR (L861Q) (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity.
Promega DDR2 Kinase Enzyme System, 10microg
Recombinant human DDR2 (467-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. DDR2 is a member of a novel subclass of RTKs containing a distinct extracellular region encompassing a factor VIII-like domain.
Promega JAK3 Kinase Enzyme System, 1mg
Recombinant human JAK3 (amino acids 781-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. JAK3 is a member of the JAK family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction.
Thermo Scientific™ beta-Galactosidase Assay Stop Solution
The Thermo Scientific™ Mammalian β-Galactosidase Assay Stop Solution is a component of the Mammalian β-Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity.
Molecular Probes™ Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit
Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit
Molecular Probes™ LIVE/DEAD™ Cell Vitality Assay Kit, C12 Resazurin/SYTOX™ Green
The LIVE/DEAD Cell Vitality Assay Kit provides a simple, two-color fluorescence assay that distinguishes metabolically active cells from injured cells and dead cells.
Molecular Probes™ SYTOX™ Blue Dead Cell Stain, for flow cytometry
SYTOX™ Blue dead cell stain is a simple and quantitative single-step dead-cell indicator for use with violet laser equipped flow cytometers.
CytoScan™ MTT Cell Proliferation Assay, 1000 reactions, G-Biosciences
CytoScan™ MTT Cell Proliferation Assay is a sensitive and accurate assay for cell proliferation and cytotoxicity. The assay is highly convenient as it is performed in a single cell tissue culture well and requires no washing, harvesting or solubilization of cells.