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Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.
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3-D Life 10x CB Buffer (pH 7 2) Phenol Red-Free (600 l) Hydrogel Component DESCRIPTION 3-D Life 10x CB-Buffer (pH 7 2) Phenol Red-Free is a component of the 3-D Life Hydrogel system In order to avoid interferences with light microscopy analysis this buffer does not contain Phenol Red It is a 10x concentrated salt and buffer solution which is diluted to physiological strength when used in the 3-D Life Hydrogel system It is used for the formation of slow gelling 3-D Life Hydrogels where it provides a cell compatible environment at pH 7 2 In conjunction with 3-D Life 10x CB-Buffer (pH 5 5) it is used to generate pHs of intermediate values to modify the gelation kinetics of fast gelling gels APPLICATION Used with other components of the 3-D Life Hydrogel System to establish 3D cell cultures QUANTITY 600 l (sufficient for at least 6 ml 3-D Life Hydrogel)
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This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes including cell division adhesion differentiation and response to stress The activity of the protein is negatively regulated by its SH3 domain whereby deletion of the region encoding this domain results in an oncogene The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation suggesting a cell cycle function This gene has been found fused to a variety of translocation partner genes in various leukemias most notably the t(9 22) translocation that results in a fusion with the 5 end of the breakpoint cluster region gene (BCR MIM 151410) Alternative splicing of this gene results in two transcript variants which contain alternative first exons that are spliced to the remaining common exons
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D-Luciferin is a popular bioluminescent substrate of luciferase in the presence of ATP used in luciferase-based bioluminescence imaging and cell-based high-throughput screening applications In an immunocompetent mouse model of ovarian cancer the use of D-luciferin substrate and firefly luciferase preserves tumour-host immune interactions since bioluminescent imaging is a more sensitive indication of tumour growth than weight gain
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SuperNuclease is a recombined Serratia marcescens’ extracellular endonuclease. The sequence is same with Benzonase (Benzonase is a trademark of Merck KGaA, Darmstadt, Germany).
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Nuc-mScarlet mRNA encodes a fusion protein combining a nuclear localization signal (Nucleus-targeting signal) with the fluorescent protein mScarlet By fusing mScarlet with a nuclear localization signal it enables the monitoring of dynamic processes inside the nucleus in living cells in real-time Nuc-mScarlet exhibits high photostability and brightness making it particularly suitable for long-term imaging experiments Moreover the spectral properties of mScarlet are compatible with other fluorescent proteins such as GFP facilitating multiplex labeling experiments EZ Cap Nuc-mScarlet Probe mRNA (m1 ) is provided at 1 mg/mL specifically designed for nuclear-targeted expression of red fluorescent protein labels This mRNA is synthesized through co-transcriptional capping resulting in a Cap 1 structure The incorporation of m1 modifications poly(A) tail significantly enhances mRNA stability and lifetime both in vitro and in vivo
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Our Streptavidin Beads T3 magnetic beads are an ideal choice for numerous applications including purification of proteins and nucleic acids protein interaction studies immunoprecipitation assays immunoassays phage display bio-screening drug screening and cell isolation Add the magnetic beads to samples containing biotinylated molecules such as peptides proteins antibodies carbohydrates lectins oligonucleotides DNA or RNA After a brief incubation the biotinylated molecules will bind to the beads and the resulting bead-molecule complexes can then be separated using a magnet The capture washing and detection steps can all be optimized for manual or automated procedures If indirect capture of a target is required first mix the biotinylated molecules with the target samples to form molecule-target complexes and then add magnetic beads to the sample
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Recombinant Tn5 Transposase is a hyperactive enzyme expressed in *E. coli* designed for efficient tagmentation-based library construction in next-generation sequencing (NGS) applications. Its rapid 5-minute reaction time allows for effective DNA fragmentation and adaptor ligation, resulting in a 9-bp target duplication. With high purity over 90%, it supports various applications such as in vitro transgenic experiments and random library construction. Ideal for researchers seeking reliable and efficient tools in genomic analysis.
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