Enzymes and Inhibitors
LGC GENOMICS LLC (Lucigen) Baseline-ZERO DNase, 5000 UDigest large or small DNA molecules better than standard DNase I.
Baseline-ZERO™ DNase digests dsDNA and ssDNA into mononucleotides more effectively than the commonly used bovine pancreatic DNase I.
New England Biolabs, Inc. Thermolabile Exonuclease I – 3000 units
Thermolabile Exonuclease I catalyzes the removal of nucleotides from single-stranded DNA (ssDNA) in the 3' to 5' direction.
Sigma Aldrich Fine Chemicals Biosciences Liberase TL Research Grade, 10 mg
Liberase TL (Thermolysin Low) Research Grade is used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high cell yield and viability. Bacterial by-products, such as endotoxins, are reduced up to several thousand-fold.
Sigma Aldrich Fine Chemicals Biosciences Amyloglucosidase from Aspergillus niger lyophilized powder, 30-60 units/mg protein (biuret), <=0.02% glucose | 9032-08-0 | MFCD00081350 | 100MG
Amyloglucosidase from Aspergillus niger lyophilized powder, 30-60 units/mg protein (biuret), <=0.02% glucose | 9032-08-0 | MFCD00081350 | 100MG
Enzyme Research Laboratories Inc Human a-Thrombin (Factor IIa),1000 U
Please use this part # if ordering a quantity of 1-9. If ordering qty 10 or more please use Fisher part # NC0704726.
Human a-Thrombin (Factor IIa),1000 U,Buffer Composition = 50 mM Sodium Citrate,0.2 M NaCl,0.1% PEG-8000,pH 6.5,Extinction Coefficient (1%) = 18.3,Molecular Weight = 37,000 daltons,Prepared from human Prothrombin by activation with Factor Xa
Medchemexpress LLC Solutol HS-15, 61909-81-7, 50 g
Formula: (C₂H₄O)nC₁₈H₃₆O3. Solutol HS-15 is a permeability enhancer. Store at 4°C, sealed storage, away from moisture.
Ankom Technology ALPHA AMYLASE 250ML
Alpha Amylase 250 ml. ANKOM FAA is a starch-hydrolyzing alpha-amylase enzyme with high heat and pH stability derived from a genetically modified strain of Bacillus licheniformis. Used for liquification of starch during Neutral Detergent Fiber Analysis.
Vector Laboratories ImmPACT™ VIP Peroxidase (HRP) Substrate Kit 120 ml
Vector Laboratories ImmPACT VIP Peroxidase (HRP) Substrate Kit produces an intense violet (purple) reaction that is 2-4 times more sensitive than that of Vector VIP Substrate. The Vector ImmPACT VIP chromogen provides an excellent color contrast in pigmented tissues such as melanoma or retina and can be used for both manual and automated staining methods. This kit consists of 120 ml of diluent and concentrated stock solutions in convenient dropper bottles. This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes. Working solutions are stable for up to 14 days at 4 degrees C. Stained sections should be dehydrated, cleared, and permanently mounted.
New England Biolabs, Inc. Template Switching RT Enzyme Mix – 20 reactions
The reverse transcriptase (RT) in the Template Switching RT Enzyme Mix adds a few non-templated nucleotides after it reaches the 5' end of the RNA template. These non-templated nucleotides can anneal to a template switching oligo (TSO) with a known sequence, prompting the reverse transcriptase to switch template from RNA to the TSO. The resulting cDNA contains a known sequence (complementary to the sequence of the TSO) attached to the 3' end. This feature can be utilized in a variety of downstream applications, such as cDNA amplification, 5' RACE (rapid amplification of cDNA ends), and 2nd strand cDNA synthesis.
Takara Bio mRNA Interferase-MazF, 1000 units
MazF is a toxin protein in the toxin-antitoxin module of E. coli. It possesses endoribonuclease activity and specifically cleaves single-stranded RNA at the 5' end of ACA sequences. This enzyme does not cleave double-stranded RNA, double-stranded DNA or single-stranded DNA.
LGC GENOMICS LLC (Lucigen) RNA 5' Polyphosphatase, 200U @ 20 U/LDephosphorylate tri- and di-phosphorylated RNA molecules while avoiding 5'-capped and mono-phosphorylated RNAs.
RNA 5 Polyphosphatase is a Mg2+-independent phosphohydrolase discovered and characterized by Epicentre scientists. The enzyme sequentially removes the and phosphates from 5-triphosphorylated RNA (such as primary RNA transcripts): 5 pppNOH 3 5 pNOH 3 + 2 Pi
Verichem Laboratories ENZYME ER VERIFIER
Enzyme ER Verifier Kit. aEc Protein-based, liquid, ready-to-use aEc Intended for calibration verification of serum kinetic assays aEc Known linear relation stated in product insert aEc Stable 14 months from date of manufacture aEc Specifically designed for extended range systems aEc assay values included for Beckman aU Chemistry Systems
Worthington Biochemical Corporation DNASE 1 100MG
Deoxyribonuclease I, Chromatographically purified, Equivalent to 1X crystallized Dnase, A lyophilized powder with glycine as a stabilizer, Store at 2 to 8degreeC, Pack Size:100mg, Source: Bovine Pancreas, min. activity: 2000U/mgKunitz dry w
New England Biolabs, Inc. BsmBI-v2 – 200 units
A Type IIS restriction endonuclease that recognizes the sequence CGTCTCN^NNNN and is optimized for Golden Gate Assembly.
- Time-Saver qualified for digestion in 5-15 minutes
- Used in Golden Gate Assembly
- Type IIS Res recognize asymmetric DNA sequences; cleave outside of their recognition site
- Supplied with 1 vial of Gel Loading Dye, Purple (6X)
Worthington Biochemical Corporation PAPAIN 100MG
Papain is a sulfhydryl protease from Carica papaya latex. It has a molecular weight of 23 kDa and an optimum pH range of 6.0-7.0. Supplied as a two times crystalline suspension in 50mM sodium acetate, pH 4.5. To insure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067mM mercaptoethanol and 5.5mM cysteine-HCl for 30 minutes. C.A.S.: 9001-73-4