Enzymes and Inhibitors
Neogen Diagnostics K-Blue Aqueous TMB Substrate, 500 mL, Microwell, HRP, Colorimetric, solvent-free
K-Blue Aqueous is a revolutionary 100% solvent-free, non-volatile TMB substrate. It contains 3,3,5,5 tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) in a one-bottle format with long-term stability and high activity. K-Blue Aqueous develops a deep blue color in the presence of peroxidase labelled conjugate and is ideal for end users working under strict regulatory requirements. The formula is compliant with Annex XVII for REACH and contains no hazardous substances at or above published reporting levels. K-Blue Aqueous has been optimized for use with microwell applications, especially ELISA. This substrate is not applicable for use with assays requiring a precipitating substrate. Stable for a minimum of 36-months when stored at 2-8C.
SCIENCELL RESEARCH LABORATORIES INC TRYPSIN NEUTRALIZATION SOL 200
NC2331260 TRYPSIN NEUTRALIZATION SOL 200
Gold Biotechnology Inc D-Luciferin, Potassium Salt (Endotoxin Free) 1g
Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.
Accelagen TURBONUCLEASE 50 000 UNITS
TurboNuclease is a recombinant Serratia macescens endonuclease (coded by the same gene as Bezonase). TurboNuclease degrades both DNA and RNA to oligonucleotides. This product is in low endotoxin, free of protease activity, and >99% pure with exceptionally high specific activity.
Worthington Biochemical Corporation Trypsin, TPCK Treated, 100mg
A chromatographically purified, diafiltered, lyophilized powder that has been treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity [Kostka, V., and Carpenter, F.: JBC, 239, 1799 (1964)]. Store at 2-8°C. Protect from moisture.
Worthington Biochemical Corporation VENOM PHOSPHODIESTERASE I
Purified by the method of Williams, Sung and Laskowski, JBC, 236, 1130 (1961). Further treated to inactivate contaminating 5'-nucleotidase activity according to Sulkowski and Laskowski: BBA, 240, 443 (1961). Lyophilized in vials.
Genscript Corporation 3C protease, His, Human
Recombinant PreScission Protease (rPSP) from human rhinovirus (HRV 3C) is a recombinant restriction-grade protease. PSP is a highly purified recombinant 6xHis-fusion protein, which recognizes the same cleavage site as the native enzyme. It is used to cleave affinity tags from fusion proteins. The optimum recognition site for this enzyme is the sequence Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro(LEVLFQ/GP) and cleavage occurs between the Gln and Gly-Pro residues. The optimal temperature for cleavage is 4°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant PreScission Protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.
Sigma Aldrich Fine Chemicals Biosciences Deoxyribonuclease I from bovine pancreas | 9003-98-9 | MFCD00130918 | 150KU
Deoxyribonuclease I from bovine pancreas | Mol Wt: ~31 kDa | 9003-98-9 | MFCD00130918 | 150KU
New England Biolabs, Inc. PvuI-HF – 500 units
A high fidelity restriction endonuclease that recognizes the sequence CG_AT^CG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
Sigma Aldrich Fine Chemicals Biosciences Glutamate Dehydrogenase (GlDH), 3000 units
Glutamate dehydrogenase has been used to measure residual ammonium by enzymatic analysis during fermentation by wine yeast. It used as a component of cofactor recycling systems for NAD(P) and NAD(P)H.
Sigma Aldrich Fine Chemicals Biosciences Liberase TL Research Grade, 10 mg
Liberase TL (Thermolysin Low) Research Grade is used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high cell yield and viability. Bacterial by-products, such as endotoxins, are reduced up to several thousand-fold.
FUJIFILM BIOSCIENCES INC LYSYL ENDOPEPTIDASE 2AU
*Orders for this supplier are non-cancellable/non-returnable once they are processed.* Lysyl Endopeptidase, Pkg. Size:1 vial (2 AU), Preparation: A 10AU-vial contains Tris-HCl corresponding to 1mL of 2mm Tris HCl buffer (pH 8.0),MW: 27,000 (gel filtration); 30,000 (SDS electrophoresis)
New England Biolabs, Inc. BsiWI-HF – 300 units
A high fidelity restriction endonuclease that recognizes the sequence C^GTACG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.