Protein Analysis Reagents
Thermo Scientific™ Pierce™ C18 Spin Tips & Columns
Thermo Scientific Pierce C18 Spin Columns (10–150 μL) and Spin Tips (10–100 μL) effectively remove interfering contaminants, while also maintaining excellent binding and recovery of peptide samples for use upstream of mass spectrometry and other high sensitivity detection methods.
Invitrogen™ Ni-NTA Agarose
Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence
Thermo Scientific™ Brij™-58 Surfact-Amps™ Detergent Solution
Purified Brij-58 detergent that is stabilized and supplied as a 10% solution in 10mL glass ampules for protein research and molecular biology.
BD Perm/Wash™ Buffer
Can be used in intracellular cytokine staining to permeabilize cells and as an antibody diluent and cell wash buffer
Novus Biologicals™ ImmEdge(R) Hydrophobic Barrier PAP Pen
provides a heat-stable, water-repellent barrier that keeps reagents localized on tissue specimens and prevents mixing of reagents when multiple sections are mounted on the same slide.
Thermo Scientific™ Pierce™ SATA (N-succinimidyl S-acetylthioacetate)
Short (2.8A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.
Thermo Scientific™ Pierce™ NEM (N-ethylmaleimide)
Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.
Tocris Bioscience™ Y-27632 in solution
Sterile-filtered 10mM solution of Y-27632 pre-dissolved in water
Thermo Scientific™ EZ-Link™ Maleimide Activated Horseradish Peroxidase
Activated horseradish peroxidase (HRP) enzyme and kit to label proteins, peptides or other ligands via sulfhydryl groups (reduced cysteines) to make HRP conjugates.
Thermo Scientific™ Metal Enhanced DAB Substrate Kit
Special formulation of diaminobenzidine peroxidase substrate that uses cobalt and nickel chloride to yield intense color for IHC staining.
Thermo Scientific™ Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO
Remove salts and small contaminants to maximize sample purity and recovery with Slide-A-Lyzer 10K MWCO dialysis cassettes.
Thermo Scientific™ Pierce™ Modified Lowry Protein Assay Kit
Clear-to-purple (A750nm), 2-component, folin phenol- and copper-based reagent system to measure total protein concentration vs. protein standard.
Thermo Scientific Chemicals Pepsin, porcine stomach
CAS: 9001-75-6 Synonym: EC 3.4.23.1; Pepsin A
Thermo Scientific™ Pierce™ Sulfo-NHS-Acetate
Amine-reactive (NHS ester) compound to permanently block primary amines (e.g., protein lysine side chains) to prevent interactions or later conjugation.
Thermo Scientific™ B-PER™ II Bacterial Protein Extraction Reagent (2X)
Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions.