Protein Analysis Reagents
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Thermo Scientific™ Pierce™ Gaussia Luciferase Glow Assay Kit
Obtain bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Gaussia luciferase reporters
| Compatible Cells | Mammalian Cells |
|---|---|
| Substrate Type | Luciferase Substrate |
| Detection Method | Bioluminescence |
| Promoter | EF-1α |
| Substrate | Coelenterazine |
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Gaussia Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
Invitrogen™ Biotin-XX, SE (6-((6-((Biotinoyl)Amino)Hexanoyl)Amino)Hexanoic Acid, Succinimidyl Ester)
Biotin-XX, SE (6-((6-((Biotinoyl)Amino)Hexanoyl)Amino)Hexanoic Acid, Succinimidyl Ester)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Acryloyl-X, SE (6-((acryloyl)amino)hexanoic acid, succinimidyl ester)
Acryloyl-X, SE (6-((acryloyl)amino)hexanoic acid, succinimidyl ester) is an amine-reactive molecule that enables crosslinking of acrylamide to proteins and other biomolecules containing amines.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Streptavidin, solid
A bacterially derived biotin-binding protein that reportedly exhibits less nonspecific binding than avidin.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ Imject™ Maleimide-Activated mcKLH
Perform hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens with this sulfhydryl-reactive mariculture KLH.
| Content And Storage | Upon receipt store product at 4°CC. Product is shipped with an ice pack. |
|---|---|
| Product Type | Imject Maleimide Activated Mariculture KLH |
| Product Line | Imject |
Thermo Scientific™ HisPur™ Cobalt Resin
Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.
| Stationary Phase | Metal Chelate, Divalent Cobalt |
|---|---|
| Type | Resin |
| Target | His-Tagged Protein |
| Content And Storage | Store at 4°C |
| Format | Bottle |
| Protein Form | Recombinant |
| Form | Liquid Suspension |
| Column Type | Affinity, Agarose Resin |
| Product Line | HisPur |
Thermo Scientific™ Pierce™ Fluorescence Biotin Quantitation Kit
Determine biotinylation levels in labeled antibodies and other proteins using this fluorescent assay reagent and HABA dye for rapid and sensitive measurement.
Thermo Scientific™ Pierce™ Fab Micro Preparation Kit
Digest human or mouse IgG antibodies with immobilized papain protease and purify Fab fragments using Protein A agarose.
Thermo Scientific™ Pierce™ Glutathione Agarose
Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.
| Stationary Phase | Glutathione |
|---|---|
| Type | Agarose |
| Content And Storage | Upon receipt store at 4°C. |
| Format | Bottle |
| Protein Form | Recombinant |
| Column Type | Affinity, Agarose Resin |
| Capacity (Metric) | 40 mg Purified Recombinant GST Protein Binding per mL Resin |
| Product Line | Pierce |
Thermo Scientific™ DyLight™ 650 NHS Ester
Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of NHS ester-activated DyLight Dyes.
| Labeling Target | Proteins |
|---|---|
| Content And Storage | Store desiccated at -20°C. |
| Product Type | Dye |
| Label or Dye | DyLight™ 650 |
| Reactive Moiety | Active Ester, Succinimidyl Ester, NHS Ester |
| Solubility | DMF (Dimethylformamide) |
| Emission | 672 |
| Excitation | 652 |
| Label Type | DyLight Fluors |
| Chemical Reactivity | Amine |
| Product Line | DyLight |
Thermo Scientific™ SM(PEG)2 (PEGylated SMCC crosslinker)
Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).
| Shipping Condition | Ambient |
|---|---|
| Content And Storage | Upon receipt store desiccated at -20°C. |
| Cleavable | No |
| Water Soluble | Yes |
| Form | Solid |
| Molecular Weight (g/mol) | 425.39 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Sulfhydryl |
| Cell Permeability | No |
| Format | Standard, Single-use |
| Product Type | Crosslinker |
| Spacer | Medium (10 to 30 Å) |
| Reactive Moiety | Maleimide, NHS Ester |
| Spacer Arm Length | 17.6 Å |
| Product Line | Pierce |
| PEGylated | Yes |
Thermo Scientific™ Pierce™ High Sensitivity Streptavidin-HRP
Amplify signal in Western blot, ELISA or other biotin-based detection methods with this exclusive, high signal-to-noise, peroxidase-conjugated, ready-to-use streptavidin.
| Content And Storage | Storage at 4°C for at least one year. |
|---|---|
| Conjugate | HRP |
| Target Molecule | Biotin |
| Concentration | 1.0–1.1 mg/mL |
| Product Line | Pierce |
Invitrogen™ pHrodo™ Dyes for Amine Labeling
Amine-reactive and TFP ester pHrodo dyes enable accurate measurement of cell endocytosis and phagocytosis, with low signal-to-background, for use in live cell assays.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Label Type | pHrodo (pH sensitive) |
| Chemical Reactivity | Amine |
| Product Line | pHrodo |
Thermo Scientific™ Pierce™ Firefly Luciferase Glow Assay Kit
Obtain bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of red firefly luciferase reporters
| Substrate | D-Luciferin |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Firefly Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Thermo Scientific™ Pierce™ Firefly Luc One-Step Glow Assay Kit
Perform firefly luciferase assays in high-throughput screening applications.
| Substrate | D-Luciferin |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Firefly Luciferase, Cypridina Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Detection Method | Bioluminescence |