Protein Mass Spectrometry Sample Prep
Waters Corp GlycoWorks RapiFluo-MS N-Glycan Eco Starter Kit, 96 Samples, UPLC Glycan BEH Column
The GlycoWorks RapiFluo-MS N-Glycan Eco Starter Kit offers a fast, sensitive method for released N-glycan analysis. It simplifies sample preparation to three steps in 30 minutes. Using RapiFluor-MS (RFMS) technology, it provides increased fluorescence and superior mass spectral response for characterization and monitoring with a single label. The “Eco” design replaces DMF with DMSO as a safer reagent solvent.
- Reduces N-glycan sample preparation to 3 easy steps in as little as 30 minutes.
- Provides increased fluorescence quantification and superior mass spectral response with RapiFluor-MS technology.
- Enables versatile labeling, using a single label for both characterization and routine monitoring.
- Replaces DMF with DMSO as a greener and safer reagent solvent, making it eco-friendly.
- Includes all necessary components for N-glycan analysis.
Waters Corp N-Glycan Eco Starter Kit, Premier UPLC Glycan BEH Column, 24 Samples
This starter kit offers a streamlined, fast, and highly sensitive method for analyzing released N-glycans. It reduces complex sample preparation to three easy steps in as little as 30 minutes. The kit includes components for deglycosylation, labeling, clean-up, sample collection, a UPLC Glycan BEH Amide Column, and Mobile-Phase Concentrate for 24 samples.
- Streamlined, fast, and highly sensitive analysis of released N-glycans.
- Sample preparation reduced to 3 easy steps in as little as 30 minutes.
- RapiFluor-MS provides increased fluorescence quantification and supreme mass spectral response.
- Single label for characterization and routine monitoring.
- Kit contains materials to process 24 samples in a 3x8 format.
Bioss Salmonellae sp.
Salmonellae sp Primary Antibody Unconjugated Host Species Mouse Target Species Clonality Monoclonal Applications ELISA Concentration 100 g/ml Size 100 ug Storage Buffer PBS (pH 7 2) with 0 1% azide
Bioss Ureaplasma sp.
Ureaplasma sp Primary Antibody Unconjugated Host Species Mouse Target Species Clonality Monoclonal Applications WB ELISA Concentration 100 g/ml Size 100 ug Storage Buffer PBS (pH 7 2) with 0 1% azide
Waters Corp GlycoWorks RapiFluor-MS N-Glycan Script Parameters Starter Kit, Automation, 96 Analyses, HILIC
The GlycoWorks RapiFluor-MS N-Glycan Script Parameters Starter Kit – Automation enables high-throughput glycoprotein sample preparation for N-glycan analysis on automated platforms. Its protocol is validated for monoclonal antibodies and diverse N-linked glycoproteins, with scaled reagents for versatile automated instrumentation.
- Includes script parameters guideline and liquid classes table to accelerate automated script development.
- Contains RFMS labeled standard, column, and mobile phase concentrate for RapiFluor-MS Library compatibility.
- Supports 96 analyses, featuring HILIC separation and MS, HRMS, MS/MS, UV detection methods.
- Components requiring cold chain shipping (deglycosylation module, standards) are clearly designated.
- Optimized for LC-MS systems in glycan applications.
Waters Corp GlycoWorks RapiFluor-MS N-Glycan Starter Kit, 24 Samples, ACQUITY Premier UPLC Glycan BEH Column
This kit provides everything necessary for enzymatic deglycosylation, RapiFluor-MS labeling, SPE clean-up, and UPLC analysis, including a UPLC column and mobile phase concentrate. It offers a streamlined, fast, and highly sensitive method for analyzing released N-glycans.
- Three easy steps in as little as 30 minutes, reducing complicated sample preparation.
- RapiFluor-MS provides increased fluorescence quantification and supreme mass spectral response.
- Uses a single label for characterization to routine monitoring.
- Processes 24 samples in a 3×8 format.
- Includes a labeling module for the solubilization and labeling of released glycosylamine.
- Features a UPLC Glycan BEH Amide column, 1.7 μm, 2.1 × 150 mm.
Waters Corp WATERS CORPORATION RapiFluor-MS N-Glycan Kit, Automation, 96 Samples
The GlycoWorks RapiFluor-MS N-Glycan Kits for automation are designed for high-throughput glycoprotein sample preparation for N-glycan analysis with minimal user intervention. The protocol is validated for monoclonal antibodies and a wide range of other N-linked glycoproteins.
- Rapid sample preparation in three steps, under an hour.
- Enhanced fluorescence quantification and superior mass spectral response with RapiFluor-MS labeling.
- Single label for characterization and routine monitoring.
- Comprehensive kits for deglycosylation, labeling, and SPE cleanup.
- Reagents scaled for automated instruments, offering platform versatility.
- Includes script information to accelerate automation results.
- “Eco” version uses DMSO as the reagent solvent.
- HILIC μElution Plate for efficient contaminant removal.
Waters Corp N-Glycan Kit, Eco Basic, 96 Samples
This GlycoWorks RapiFluor-MS N-Glycan Eco Kit simplifies and accelerates glycan analysis sample preparation, reducing it to 30 minutes in three easy steps. Utilizing RapiFluor-MS (RFMS) for enhanced fluorescence quantification and superior mass spectral response, it allows a single label for characterization and routine monitoring. The “Eco” version uses greener DMSO as a reagent solvent, replacing DMF.
- Reduces sample preparation to 30 minutes in 3 easy steps.
- Enhanced detection provides increased fluorescence quantification and supreme mass spectral response.
- Single labeling technology can be used for both glycan characterization and routine monitoring.
- Eco-friendly as it replaces DMF with DMSO as a greener and safer solvent.
- Includes deglycosylation, eco labeling, and eco clean-up modules.
Aat Bioquest HIS Lite™ iFluor™ 647 Tris NTA Chelator, 100 ug
iFluor® 647 Tris-NTA Chelator is used as a sensitive red fluorescent probe for detecting polyhistidine-labeled proteins in combination with the addition of a heavy metal ion such as Ni2+ in cells, solution and solid surfaces. Fluorescent tris-NTA compounds provide an efficient method for site-specific and stable noncovalent fluorescence labeling of polyhistidine-tagged proteins. In contrast to the transient binding of conventional mono-NTA, the multivalent interaction of tris-NTA conjugated fluorophores form a much more stable complex with polyhistidine-tagged proteins. The high selectivity of tris-NTA compounds toward cumulated histidines enables the selective labeling of proteins in cell lysates and on the surface of live cells. Fluorescent tris-NTA conjugates can be applied for the analysis of a ternary protein complex in solution and on surfaces.