Protein Purification Reagents and Kits
MilliporeSigma Total Protein Extraction Kit, 1 kit
Total protein extraction kit is an excellent tool for the initial purification and preparation of proteins from any tissues and cells. Total protein isolated by this kit is native and can be used for downstream applications.
Immunology Consultants anti-DYKDDDDK (Flag) Antibody Rabbit - HRP Conjugated
RFLG‑45P is an HRP‑conjugated rabbit antibody specific for the DYKDDDDK (FLAG) tag, designed for chemiluminescent and colorimetric detection workflows. The horseradish peroxidase label enables highly sensitive signal generation in ELISA, Western blotting, and other enzyme‑based assays. Affinity purification ensures strong specificity and minimal nonspecific binding, supporting consistent and reproducible results. Produced by Immunology Consultants and supplied in a 1 mg format, RFLG‑45P is a powerful reagent for robust, high‑sensitivity FLAG detection.
Tosoh Bioscience LLC TSKGEL OCTADECYL-4PW 4.6X150
Column, Reversed Phase Chromatography; Tosoh TSKgel Octadecyl-4PW; Media: Polymer; Particle: 7 micrometer; Pore Size: 500 angstrom; 4.6mm I.D. x 15cm Length; Stainless Steel
Immunology Consultants anti-DYKDDDDK (Flag) Antibody Rabbit - Affinity Purified
RFLG‑45A is an affinity‑purified rabbit polyclonal antibody targeting the DYKDDDDK (FLAG) epitope, widely used in recombinant protein expression and detection. The purification process yields high‑specificity antibodies with minimal background, supporting clear and reproducible results in Western blotting, ELISA, immunoprecipitation, and immunofluorescence. This reagent provides strong, selective binding to FLAG‑tagged proteins, ensuring dependable performance across diverse assay conditions. Manufactured by Immunology Consultants and supplied in a 1 mg format, RFLG‑45A is an essential tool for researchers working with FLAG‑tagged constructs.
Zymo Research Corporation M-Solubilization Buffer (4.5 ml)
The M-Solubilization Buffer is designed for use in the preparation of the CT Conversion Reagent in the EZ DNA Methylation-Direct Kit. The M-Solubilization Buffer facilitates the solubilization of the CT Conversion Reagent from a solid mixture into a liquid reagent. Highlights: Sold separately or as part of the EZ DNA Methylation-Direct Kits.
Zymo Research Corporation EZ DNA Methlyation-Direct™ Kit (50 Rxns)
The EZ DNA Methylation™-Direct™ Kit is a further refinement of our popular EZ DNA Methylation™ and EZ DNA Methylation™-Gold™ kits. These products feature reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of these kits make it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Like the EZ DNA Methylation™-Gold™ kits, DNA denaturation and bisulfite conversion processes are combined into a single step. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.
Enzo Life Sciences Poly(ADP-ribose), standard (1µg)
Alternative name: PAR. Purity: ≥95% (HPLC of ADP-ribose RNA and DNA measurement). MW: 1’100 to 275’000 depending on the number of units of ADP-ribose. Formulation: Liquid. In water. Source: Released from automodified PARP-1 by base treatment and purified by boronate affinity. Long Term Storage: -80°C. Use/Stability: Stable for one week when stored at +4°C. Stable for at least 2 years after receipt when stored at -80°C. Handling: Avoid freeze/thaw cycles.
ATHENA ES 100 mM TTAB
Detergents have been shown to aid in the protein refolding process both of native and recombinant proteins. Detergents are usually used as a step in the protein refolding process, often in conjunction with cyclodextrins. The detergent binds to the protein in the dilution stage to form a detergent-protein complex which prevents aggregation of the protein. A binding agent, such as cyclodextrin, can then be used to strip away the detergent from the protein, allowing it to properly refold. Athena’s Detergent Screening Kit provides researchers with 6 common detergents (10mL each) for determining the optimum detergent for a given protein refolding scenario. Individual detergents can also be purchased in 100mL quantities.
Antibodies Online MagSi-S Epoxy 3.0 Beads, 2ml
PurposeSuperparamagnetic silica particles with epoxy modified surface. Intended for epoxy coupling chemistry to enzymes and other NH2 containing molecules.Bead Size - 3 µmCharacteristicsCoating chemistry: EpoxyBead concentration: 1 - 3 x 10^9 beads/mLComponentsMagnetic silica beads with activated surface
McMaster-Carr 3D-Printed Part Removal Tool, 4 1/4"L
Separate 3D-printed parts from 3D-printer plates without damaging either one of them. Unlike conventional putty knives or spatulas, these tools have a right-angle handle and tapered blade, which makes it easier to slide them under parts and loosen them from the plates. The blade’s rounded edge prevents nicks and scrapes. The rubber handle gives you a nonslip grip.
ATHENA ES 100 mM POE(10)L
Detergents have been shown to aid in the protein refolding process both of native and recombinant proteins. Detergents are usually used as a step in the protein refolding process, often in conjunction with cyclodextrins. The detergent binds to the protein in the dilution stage to form a detergent-protein complex which prevents aggregation of the protein. A binding agent, such as cyclodextrin, can then be used to strip away the detergent from the protein, allowing it to properly refold. Athena’s Detergent Screening Kit provides researchers with 6 common detergents (10mL each) for determining the optimum detergent for a given protein refolding scenario. Individual detergents can also be purchased in 100mL quantities.
Invent Biotechnologies Inc Minute™ SDS-Remover (10 ml)
This item has a minimum qty of 3 per supplier requirements.
Sodium dodecyl sulfate is one of the most commonly used detergents for biological research. However, the presence of SDS in the sample could interfere with downstream applications. For instance, SDS must be reduced to below a certain concentration before trypsin digestion of protein can be performed effectively. The presence of SDS in a protein sample could interfere with, and needs to be removed prior to, the analysis. The presence of SDS in protein samples also interferes with antigen-antibody binding and has a negative impact for experiments such as immunoprecipitation and ELISA. This proprietary formulated reagent is designed for fast and easy removal of SDS in solution by precipitation. The efficacy is superior to SDS-removal column and TCA precipitation with minimum protein loss (<20%). Due to the small volume of SDS-Remover used, the final protein concentration of the sample is not significantly impacted.