Protein Purification Reagents and Kits
STEMCELL Technologies EasySep™ Direct Human Neutrophil Isolation Kit, For processing: 100 mL whole blood processed
The EasySep™ Direct Human Neutrophil Isolation Kit isolates functional, highly purified neutrophils directly from human whole blood by immunomagnetic negative selection. No lysis, density gradient centrifugation or other processing steps are required and isolated cells are immediately available for flow cytometry, functional assays, culture, and other downstream applications.This product replaces the EasySep™ Human Neutrophil Isolation Kit (Catalog #19257) for even faster cell isolations.
Sigma Aldrich Fine Chemicals Biosciences COMPLETE HIS-TAG PURIFICATION
COHISC-RO Roche cOmplete His-Tag Purification
STEMCELL Technologies RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail, For processing: 1000 mL cord blood processed
The RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Kit is designed to pre-enrich hematopoietic progenitor cells from cord blood by negative selection. Unwanted cells are targeted for removal with antibody complexes recognizing CD3, CD11b, CD19 and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The hematopoietic progenitor cells are present as an enriched population at the interface between the plasma and the buoyant density medium. Cells of interest can then be positively selected from the pre-enriched cell population using EasySep™ (e.g. EasySep™ Human CD34 Positive Selection).
STEMCELL Technologies RoboSep™ Mouse NK Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse NK Cell Isolation Kit targets non-NK cells by labeling unwanted cells with biotinylated antibodies and EasySep™ Streptavidin RapidSpheres™, and separates cells without columns using an EasySep™ magnet. Desired cells are simply poured off into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry, culture or cell-based assays.This product replaces the EasySep™ Mouse NK Cell Enrichment Kit (Catalog #19755) for even faster cell isolations.
STEMCELL Technologies RosetteSep™ Human T Cell Enrichment Cocktail, For processing: 40 mL whole blood processed
The RosetteSep™ Human T Cell Enrichment Cocktail is designed to isolate T cells from whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-T cells and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep™ DM-L (Catalog #15705) or Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified T cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium.
Cell Biolabs Inc QuickTiter™ Lentivirus Quantitation Kit, 20 assays
Cell Biolabs' QuickTiter™ Lentivirus Quantitation Kit provides an ultra-fast 45-60 minute procedure that measures physical titer of purified virus or unpurified supernatant via the viral nucleic acid content. Quantitation is performed on a 96-well fluorescence based plate reader. The kit provides sufficient reagents for 20 assays.
Ubpbio GLUTATHIONE AGAROSE, 10ML
Protein Purification Reagents – Glutathione Resin (UBPBio catalog# P3060-10, P3060-100) – Glutathione Xpure Agarose Resin consists of glutathione covalently bound to agarose beads. This resin has high binding affinity and specificity to the Glutathione S-transferase (GST) protein. GST-tagged proteins can be highly purified by batch or column purification (low flow rate) in a one step purification method. Bound GST-tagged proteins are easily eluted from the resin with buffers containing reduced glutathione.
STEMCELL Technologies EasySep™ Direct Human NK Cell Isolation Kit, For processing: 100 mL whole blood processed
The EasySep™ Direct Human NK Cell Isolation Kit is designed to isolate NK cells from human whole blood by immunomagnetic negative selection. The EasySep™ Direct procedure involves labeling RBCs, platelets and unwanted cells present in human whole blood with antibody complexes and magnetic particles. The magnetically labeled RBCs, platelets and cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
STEMCELL Technologies EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II, For processing: 1 x 10^9 cells
The EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II is designed to isolate CD4+CD25+ T cells from single-cell suspensions of splenocytes or other tissues by negative followed by positive selection. CD4+ T cells are first enriched by negative selection using the EasySep™ Mouse CD4+ T Cell Isolation Cocktail. CD25+ cells are then positively selected from the pre-enriched cells using EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Cocktail.
STEMCELL Technologies EasySep™ Mouse CD4 Positive Selection Kit II, For processing: 2 x 10^9 cells
The EasySep™ Mouse CD4 Positive Selection Kit II is designed to isolate CD4+ cells from single-cell suspensions of splenocytes or other tissues by positive selection. Desired cells are targeted with antibody complexes recognizing CD4 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Mouse CD4 Positive Selection Kit (Catalog #18752) for even faster cell isolations and does not result in the labeling of isolated cells with PE.
STEMCELL Technologies EasySep™ Human Pan-Granulocyte Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Pan-Granulocyte Enrichment Kit is designed to isolate granulocytes (eosinophils, basophils and neutrophils) from normal peripheral whole blood by negative selection. Undesired cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-granulocytes and magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.