Tissue Lysates
Sino Biological Recombinant Human Alkaline Phosphatase/ALPL Protein (AVI Tag), Avi-tag Biotinylated, HPLC-verified
A DNA sequence encoding the Human ALPL (NP_000469.3) (Met1-Ser502) was fused with an AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Sino Biological Protein NRG1 ET-FREE 100UG
A DNA sequence encoding the N-terminal fragment (Ser 2-Lys 246) of human NRG1 isoform beta1 (Q02297-6) was fused with the Fc region of human IgG1 at the N-terminus
Sino Biological Protein LGR5 Nanodisc 100UG
A DNA sequence encoding the Human LGR5 (O75473) (Gly22-Ser844) was expressed with a flag tag at the N-terminusand a C-terminal polyhistidine tag followed by an AVI tag The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed This protein was prepared using our Nanodisc platform Nanodisc is a versatile tool for studying membrane proteins Using styrene-maleic acid (SMA) copolymer membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better Compared to membrane scaffold proteins (MSPs) nanodiscs SMA nanodiscs also have the advantage of preserving proteins nature by maintaining native lipids surrounded without introducing any heterologous proteins which allows studies of protein structure and functions in a native-like environment
Sino Biological Protein Mouse TNC 100UG
A DNA sequence encoding the Mouse TNC (Q80YX1) (Pro626-Leu1077) was expressed with a polyhistidine tag at the C-terminus