Restriction Enzymes
New England Biolabs, Inc. WarmStart Nt.BstNBI-1000 u
WarmStart Nt.BstNBI is a site specific nicking endonuclease cloned fromBacillus Stereothermophilus. Restriction Enzyme Cut Site GAGTC(4/-5). It is formulated with a reversibly-bound aptamer which inhibits its nicking activity at temperatures below 40C. WarmStart Nt.BstNBI cleaves only one strand of DNA on a double-stranded DNA substrate. The nicking endonuclease catalyzes a single strand break 4 bases beyond the 3side of the recognition sequence.
New England Biolabs, Inc. EcoRI – 10000 units
A restriction endonuclease that recognizes the sequence G^AATT_C.
New England Biolabs, Inc. I-CeuI – 500 units
A restriction endonuclease that recognizes the sequence CGTAACTATAACGGTC_CTAA^GGTAGCGAA.The intron encoding I-CeuI is present in the chloroplast large rRNA gene of Chlamydomonas eugametos (1). This gene has been cloned and overexpressed in E. coli (2).
New England Biolabs, Inc. ApoI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence R^AATTY. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. BtsI-v2 – 2500 units
BtsI-v2 is an engineered and improved version of BtsI that recognizes the sequence GCAGTGNN^.
New England Biolabs, Inc. MspJI – 200 units
A restriction endonuclease that recognizes the sequence CNNRNNNNNNNNN^NNNN_.
New England Biolabs, Inc. PaqCI - 1000 units
PaqCI is a Type IIS restriction enzyme with a 7 basepair recognition sequence: CACCTGC(4/8). It is sourced from an E. coli strain that carries the modified cloned PaqCI gene from Paucibacter aquatile. PaqCI requires multiple sites for efficient cleavage -- PaqCI Activator is included for optimal cleavage. This enzyme is supplied with NEB's rCutSmart Buffer and is heat inactivated.
New England Biolabs, Inc. BsrBI – 1000 units
A restriction endonuclease that recognizes the sequence CCG_^CTC.
New England Biolabs, Inc. SfiI – 15000 units
A restriction endonuclease that recognizes the sequence GGCCN_NNN^NGGCC.
New England Biolabs, Inc. NlaIII – 500 units
A restriction endonuclease that recognizes the sequence _CATG^.
New England Biolabs, Inc. FokI – 5000 units
A restriction endonuclease that recognizes the sequence GGATGNNNNNNNNN^NNNN_.
New England Biolabs, Inc. HpaI – 500 units
A restriction endonuclease that recognizes the sequence GTT_^AAC.
New England Biolabs, Inc. HaeII – 2000 units
A restriction endonuclease that recognizes the sequence R_GCGC^Y.
New England Biolabs, Inc. NsiI – 5000 units
A restriction endonuclease that recognizes the sequence A_TGCA^T.
New England Biolabs, Inc. AatII – 2500 units
A restriction endonuclease that recognizes the sequence G_ACGT^C.