Restriction Enzymes
New England Biolabs, Inc. BmrI – 100 units
A restriction endonuclease that recognizes the sequence ACTGGGNNNN_N^.
New England Biolabs, Inc. XbaI – 15000 units
A restriction endonuclease that recognizes the sequence T^CTAG_A.
New England Biolabs, Inc. PflFI – 2000 units
A restriction endonuclease that recognizes the sequence GACN^N_NGTC.
New England Biolabs, Inc. SspI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence AAT_^ATT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. NruI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence TCG^CGA. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. AvrII – 100 units
A restriction endonuclease that recognizes the sequence C^CTAG_G.
New England Biolabs, Inc. DpnI – 1000 units
A restriction endonuclease that recognizes the sequence GA_^TC.
- DpnI cleaves only when its recognition site is methylated
- Cleavage of mammalian genomic DNA is blocked by overlapping CpG methylation
- Methylation-sensitive restriction enzyme
- Time-Saver qualified for digestion in 5-15 minutes
- 100% activity in rCutSmart Buffer (over 210 enzymes are available in the same buffer)
New England Biolabs, Inc. BspDI – 2000 units
A restriction endonuclease that recognizes the sequence AT^CG_AT.
New England Biolabs, Inc. NsiI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence ATGCA^T. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. NsiI – 1000 units
A restriction endonuclease that recognizes the sequence A_TGCA^T.
