Cell Based Assays

BD Annexin V: PE Apoptosis Detection Kit I Available on GSA/VA Contract for Federal Government customers only.

Contains apoptotic program characterized by certain morphologic features, including loss of plasma membrane asymmetry and attachment, condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA

R&D Systems NeuroPore Permeabilization and Blocking Reagent

Provides gentle permeabilization and facilitates access to the DNA for the biotinylated dNTPs where CNS tissues will not withstand aggressive protease treatment

R&D Systems TACS TdT In Situ Apoptosis Detection Kit, Blue Label

A TUNEL assay designed for fixed cells, embedded tissue, and frozen tissue using light and electron microscopy

R&D Systems CometSlide™ Microscope Slides

Sample-ready pretreated glass slides used for the rapid analysis of DNA fragmentation associated with DNA damage

R&D Systems TACS 2 TdT Replinisher Kit

Provides only reagents most rapidly exhausted from the complete TACS 2 TdT kits

Tocris Mixed NMDA Receptor Tocriset™

Can be used to investigate the pharmacology of NMDA receptors

R&D Systems Caspase-9 Colorimetric Assay Kit

Plate-based assay uses a peptide coupled to a colorimetric reporter system to assess activity in cell lysates

BD Pharmingen™ DRAQ7™

Used to stain nucleic acids in fixed cells. BD Pharmingen™ DRAQ7™ is also used for cell cycle analysis by DNA content, nuclear visualization, or discrimination of nucleated cells from debris or neucleated cells.

R&D Systems MTT Cell Proliferation/Viability Assay

Safe and reliable method minimizes the number of steps necessary to assay for proliferation rate in cultured cells

Novus Biologicals™ Annexin V Apoptosis Kit, FITC, with Control Cells

Use with flow cytometry to identify apoptotic cells.

BD Cell Viability Solution Available on GSA/VA Contract for Federal Government customers only.

Ready-to-use solution of the nucleic acid dye, 7-Amino-actinomycin D (7-AAD) that can be used in place of propidium iodide (PI) for the exclusion of nonviable cells in flow cytometric assays

R&D Systems TACS Annexin V-FITC Apoptosis Detection Kit

Facilitates rapid fluorometric quantitation of apoptotic cells

R&D Systems PARP Universal Colorimetric Assay Kit

Measures the activity of Poly (ADP-ribose) Polymerase (PARP) in cells and tissues by detecting the incorporation of biotinylated Poly (ADP-ribose) (PAR) onto histone proteins coating a 96-stripwell microplate

Tocris β-Adrenoceptor Antagonist Tocriset™

Can be used to inhibit β1, β2 and β3-adrenoceptors

BD Phospholipid-binding Protein, APC Annexin V Available on GSA/VA Contract for Federal Government customers only.

Has high affinity for PS, and binds to cells with exposed PS

BD Apoptosis, DNA Damage and Cell Proliferation Kit

Designed with the inclusion of fluorescent antibodies specific for incorporated BrdU, phosphorylated H2AX (γH2AX) and cleaved PARP

BD Annexin V-FITC Available on GSA/VA Contract for Federal Government customers only.

Serves as a sensitive probe for flow cytometric analysis of cells that are undergoing apoptosis

R&D Systems Caspase-3 Fluorometric Assay Kit

Plate-based assay uses a peptide coupled to a fluorometric reporter system to assess activity in cell lysates

R&D Systems CometAssay™ High Throughput Slides

Provides convenient, simple and reliable sample preparation, processing, analysis and storage

R&D Systems TACS Annexin V-Biotin Apoptosis Detection Kit

Offers flexibility in labeling by allowing the use of streptavidin-conjugated fluorophores other than FITC for detection

BD Annexin V: FITC Apoptosis Detection Kit II Available on GSA/VA Contract for Federal Government customers only.

Staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic processes.

R&D Systems Caspase-8 Colorimetric Assay Kit

Plate-based assay uses a peptide coupled to a colorimetric reporter system to assess activity in cell lysates

BD Pharmingen™ DRAQ5™

Used to stain nucleic acids in live or fixed cells. BD Pharmingen™ DRAQ5™ is also used for cell cycle analysis by DNA content, nuclear visualization, or discrimination of nucleated cells from debris or enucleated cells.

R&D Systems Cytonin

Use in dual-labeling experiments detecting both DNA fragmentation and antigens that may be sensitive to proteinase K treatment. Cytonin can be used to permeabilize cells and tissues prior to labeling with TACS™ in situ Apoptosis Detection Kits.

BD FAS:FC Chimeric Fusion Cell Surface Protein Available on GSA/VA Contract for Federal Government customers only.

Mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand

BD Caspase-3 Active Form, Recombinant Human Protein Available on GSA/VA Contract for Federal Government customers only.

Play a key role in cell apoptosis

Tocris MAPK Inhibitor Tocriset™

Can be used to study MAPK signaling

BD Caspase-3 Assay Kit Available on GSA/VA Contract for Federal Government customers only.

Designed to measure caspase-3 or DEVD-cleaving activity an marker of cells undergoing apoptosis.

R&D Systems TACS-XL In Situ Apoptosis Detection Kits

A TUNEL assay designed for fixed cells, embedded tissue, and frozen tissue using light and electron microscopy

Tocris Serotonin Uptake Inhibitor Tocriset™

Can be used to modulate serotonin re-uptake

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