CD81 Monoclonal Antibody (Eat2), Invitrogen™

Armenian Hamster Monoclonal Antibody

Supplier:  Thermo Scientific MA180209

Catalog No. MA180209

Description

This antibody induces homotypic aggregation of B cells and inhibits anti-Ig and IL-4 induced proliferation, and requires the presence of both extracellular loops of TAPA-1 for binding. The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Hamster anti Mouse CD81 antibody, clone Eat2 recognizes mouse and rat CD81, also known as TAPA-1 or Target of the antiproliferative antibody 1.

CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells. In muscles, CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that is known to complex with integrins. CD81 appears to promote muscle cell fusion and support myotube maintenance. CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.

Specifications

• Antigen: CD81
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Gene Accession No.: P35762, Q62745
• Gene Symbols: CD81
• Immunogen: 38C13, murine B cell line.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
• Clone: Eat2
• Conjugate: Unconjugated
• Gene: CD81
• Gene Alias: 26 kDa cell surface protein TAPA-1; CD 81 antigen; Cd81; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1); CD81 molecule; CVID6; S5.7; Tapa1; Tapa-1; Target of the antiproliferative antibody 1; Tetraspanin28; tetraspanin-28; Tspan28; Tspan-28
• Host Species: Armenian Hamster
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 12520, 25621
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid