MP Biomedicals™ SPINeasy™ Plasmid Midiprep Kit

Catalog No. MP116539025

Supplier: MP Biomedicals™ 116539025

Description

Spin column technology for fast and efficient purification of both high and low copy plasmids from bacterial cultures. High-quality plasmid DNA is free of gDNA contamination following a modified alkaline lysis method.

• SPINeasy Plasmid Midiprep Kit is a silica membrane spin column kit for the purification of high-copy and low-copy number plasmid DNA from 25 to 50 mL of transformed bacterial cultures.
• Using our specially formulated buffers, bacterial cells are disrupted by alkaline lysis to release plasmid DNA.
• Cell debris, such as chromosomal DNA and proteins, are precipitated by neutralization and cleared through centrifugation. Subsequently, plasmid DNA in the supernatant is adsorbed on a silica membrane, washed, and eluted in a convenient process using spin column technology.
• Up to 1 mg of plasmid DNA can be purified through this simple workflow, which is immediately ready for routine molecular biology applications, including cloning, in-vitro transcription, PCR, and restriction digestion.

Specifications

• Content And Storage: Store at Room Temperature, Dry Environment
• Isolation Technology: Spin Column
• Sample Type: 25 to 50 mL Bacterial (E. coli) Cultures
• Includes: 60 mL Resuspension Buffer SD1, 60 mL Alkaline Lysis Buffer SD2, 90 mL Neutralization Buffer SD3, 70 mL Wash Buffer DW1, 12 mL Wash Buffer DW2, 30 mL Elution Buffer SD, 600 µL RNase A, 25 x Column SD with collection tubes
• Quantity: 25 Preps

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use.