General Western Blot Reagents
Alkali Scientific PVDF Membrane Stripping Buffer 4X Solution, 500 Milliliters
PVDF Membrane Stripping Buffer 4X Solution is designed for Western blotting applications where stripping and re-probing of PVDF membranes are required. This 4X concentrated solution effectively removes bound antibodies from the membrane without damaging the protein or membrane integrity. It is ideal for stripping multiple antibodies from the same blot for re-probing with different antibodies. The concentrated formulation allows for the use of smaller volumes while maintaining effective stripping. This buffer is essential for researchers performing multiple antibody assays on the same PVDF membrane, saving time and resources in protein analysis.
Seracare Life Sciences Inc SILVER ENHANC KIT-MEMBRANES
KPL's Silver Enhancer Kits are available for use in membrane based applications. This simple yet sensitive system is optimized for use with KPL's gold conjugates for the amplification of gold particles. Silver builds up on the surface of the gold particles as a result of the reduction of silver from one solution to another. Silver Enhancer kits offer low background, sensitivity to the picogram level and slow, controlled development. 15-25 minutes development for microscopy applications.
ABclonal Technology Cytochrome P450 2E1 CYP2E1
This gene encodes a member of the cytochrome P450 superfamily of enzymes The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids This protein localizes to the endoplasmic reticulum and is induced by ethanol the diabetic state and starvation The enzyme metabolizes both endogenous substrates such as ethanol acetone and acetal as well as exogenous substrates including benzene carbon tetrachloride ethylene glycol and nitrosamines which are premutagens found in cigarette smoke Due to its many substrates this enzyme may be involved in such varied processes as gluconeogenesis hepatic cirrhosis diabetes and cancer
ProteinSimple 66-440 kDa Pre-filled Plates
Compatible with Jess, Abby and Wes systems using Compass v2.7 or higher. Includes 8 Plates (pre-filled with Separation Matrix, Stacking Matrix, Split Running Buffer 3 and Matrix Removal Buffer).
Vector Laboratories Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II) Biotinylated 2 mg
Griffonia simplicifolia lectin II (GSL II) is a dimeric glycoprotein composed of two subunits of nearly identical size. Each subunit has disulfide-linked chains and a binding site for α- or β-linkedN-acetylglucosamine residues. Unlike other N-acetylglucosamine-specific lectins, increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. GSL II is unique in its ability to recognize exclusively α- or β-linked N-acetylglucosamine residues on the nonreducing terminal of oligosaccharides. This conjugate features a ratio of biotin to lectin protein that provides optimal avidin/streptavidin and lectin binding activity. Supplied essentially free of unconjugated biotins, it is preserved with sodium azide.
ProteinSimple 12-230 KDA WES SEPARATION MODU
12-230 kDa Wes Separation Module, 2 x 25 capillary cartridges.Use with a Detection Module. Reagents for 50 data points includes two parts: Separation box with 2x Capillary Cartridges (25 capillaries per cartridge), 2x Wes 12-230 kDa pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 2 and Matrix Removal Buffer), Sample Buffer, Wash Buffer (Store at RT), plus 1x Standard Pack 1 clamshell with 2x DTT and 2x FL master mix with 29 kDa System Control protein. (Store at 4 C).
Alkali Scientific Blot Stripping Buffer 4X Solution, 500 Milliliters
Blot Stripping Buffer 4X Solution is a powerful reagent for the efficient removal of antibodies or proteins from membranes, making them suitable for reuse in subsequent Western blot experiments. This 500mL bottle provides a large quantity of buffer ideal for labs conducting high-throughput analysis or multiple rounds of probing. The buffer’s 4X concentration ensures that it effectively strips the membrane while maintaining its integrity for future use. It is commonly employed in protein analysis experiments, where stripping and re-probing the same membrane for different target proteins are necessary, offering a cost-effective solution for repeated analysis.
Sigma Aldrich Fine Chemicals Biosciences Peroxidase from horseradish Type I, essentially salt-free, lyophilized powder, >=50 units/mg solid (using pyrogallol) | 9003-99-0 | MFCD00071339 | 5KU
Peroxidase from horseradish Type I, essentially salt-free, lyophilized powder, >=50 units/mg solid (using pyrogallol) | 9003-99-0 | MFCD00071339 | 5KU
ABclonal Technology Cytochrome P450 2E1 CYP2E1
This gene encodes a member of the cytochrome P450 superfamily of enzymes The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids This protein localizes to the endoplasmic reticulum and is induced by ethanol the diabetic state and starvation The enzyme metabolizes both endogenous substrates such as ethanol acetone and acetal as well as exogenous substrates including benzene carbon tetrachloride ethylene glycol and nitrosamines which are premutagens found in cigarette smoke Due to its many substrates this enzyme may be involved in such varied processes as gluconeogenesis hepatic cirrhosis diabetes and cancer