Cell Based Assays
Promega ADP-Glo™ Kinase Assay + MLCK Kinase Enzyme System
Recombinant human mLCK (1425-1776) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. mLCK or myosin light chain kinase is a muscle member of the immunoglobulin gene superfamily and a calcium/calmodulin-dependent protein kinase.
Promega ADP-Glo™ Kinase Assay + TNIK Kinase Enzyme System
Recombinant human TNIK (1-367) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TNIK or TRAF2 and NCK-interacting kinase are characterized by N-terminal kinase and C-terminal GCK domains that serve a regulatory function.
Promega AMPK (A1/B1/G1) Kinase Enzyme System, 1mg
Recombinant full-length human AMPK (combination of A1/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using C-terminal His tags. AMPK is an important energy-sensing enzyme that monitors cellular energy status.
Promega ADP-Glo™ Kinase Assay + FLT3 Kinase Enzyme System
FLT3 is a receptor tyrosine kinase that has been shown to play a role in proliferation and survival of hematopoietic progenitor cells as well as differentiation of early B lymphoid progenitors.
Promega ADP-Glo™ Kinase Assay + DNA-PK Kinase Enzyme System
Native human DNA-Dependent Protein Kinase purified from HeLa cells. Consists of a ∼460kDa catalytic subunit and a heterodimeric DNA-binding subunit (Ku) containing an 85kDa and a 70kDa peptide (1). Gene sequence can be found at accession number NM_006904.
Promega CAMK1 gamma Kinase Enzyme System, 1mg
Full-length recombinant human CAMK1 gamma was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CAMK1 gamma (CLICK-III), a member of the CAMK family, is a novel membrane-anchored neuronal Ca2+/calmodulin-dependent protein kinase.
Promega MAPKAPK5 Kinase Enzyme System, 1mg
Recombinant full-length human MAPKAPK5 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MAPKAPK5 is a member of the serine/threonine kinase family that responds to cellular stress and proinflammatory cytokines.
Promega ADP-Glo™ Kinase Assay + PAK1/CDC42 Kinase Enzyme System
Recombinant full-length human PAK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag and combined with CDC42 at a ratio of 1:4 (PAK1:CDC42) in vitro. PAK1 is a member of the p21-activated kinases (PAKs).
Promega PDGFR alpha (T674I) Kinase Enzyme System, 10microg
Recombinant human PDGFRα (Y674I) (550-end) point mutant was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega TAOK1 Kinase Enzyme System, 10microg
TAOK1 is a serine/threonine-protein kinase involved in regulation of the p38-containing stress-responsive MAP kinase pathway and extracellular signal-regulated protein kinase (ERK) kinases (MEKs).
Promega FES Kinase Enzyme System, 1mg
Recombinant full-length human FES was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FES is a proto-oncogene that encodes a protein-tyrosine kinase distinct from c-Src, c-Abl and other nonreceptor tyrosine kinases.
Promega EGFR (T790M) Kinase Enzyme System, 10microg
Recombinant human EGFR (T790M) (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity.
Promega FYN A Kinase Enzyme System, 1mg
Full-length recombinant human FYN A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FYN A (Fyn isoform A) is a member of the SRC tyrosine kinase oncogene family showing high homology to YES1, FGR and SRC.
Promega CAMK2 gamma Kinase Enzyme System, 1mg
Recombinant human CAMK2 gamma (C-terminal truncation) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. CAMK2 gamma is a member of the CAMKII family.
Promega GRK5 Kinase Enzyme System, 10microg
Recombinant full-length human GRK5 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. GRK5 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family.