Enzymes and Inhibitors
New England Biolabs, Inc. TaqI-v2 – 4000 units
A restriction endonuclease that recognizes the sequence T^CG_A.
New England Biolabs, Inc. NEBuffer™ 1 – 5 ml
New England Biolabs provides a color-coded 10X NEBuffer (with BSA) with each restriction endonuclease to ensure optimal (100%) activity. This NEBuffer minus BSA formulation is available for those enzymes requiring NEBuffers without BSA. For the BSA containing formulation (which is included with NEB restriction enzymes), select NEBuffer 1.1. New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. The NEBuffer minus BSA formulation is included in this buffer pack for those enzymes requiring NEBuffers without BSA. For the BSA containing formulation, Buffer packs NEBuffer 1.1 (NEB #B7201), NEBuffer 2.1, NEBuffer 3.1 and CutSmart Buffer (NEB #B7204) are available.
New England Biolabs, Inc. NdeI – 20000 units
A restriction endonuclease that recognizes the sequence CA^TA_TG.
New England Biolabs, Inc. I-SceI – 500 units
A restriction endonuclease that recognizes the sequence TAGGG_ATAA^CAGGGTAAT. I-SceI is an intron-encoded endonuclease. The intron encoding I-SceI is present in the mitochondria of Saccharomyces cerevisiae.
New England Biolabs, Inc. EcoRV – 20000 units
A restriction endonuclease that recognizes the sequence GAT_^ATC.
New England Biolabs, Inc. AvaI – 2000 units
A restriction endonuclease that recognizes the sequence C^YCGR_G.
New England Biolabs, Inc. MlyI – 1000 units
A restriction endonuclease that recognizes the sequence GAGTCNNNNN_^.
New England Biolabs, Inc. RsaI – 5000 units
A restriction endonuclease that recognizes the sequence GT_^AC.
New England Biolabs, Inc. HpaII – 10000 units
A restriction endonuclease that recognizes the sequence C^CG_G.
Supply Solutions Liberase™ TH Research Grade, 2x5mg
Liberase TH Research Grade contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other and with a high concentration of Thermolysin, a non-clostridial neutral protease.
Supply Solutions Roche - RNA Polymerase, T7, 1000 U; from Escherichia coli BL 21/pAR 1219; pkg of 1,000 U (10881767001) pkg of 5,000 U (10881775001)
Roche - RNA Polymerase, T7, 1000 U; from Escherichia coli BL 21/pAR 1219; pkg of 1,000 U (10881767001) pkg of 5,000 U (10881775001)
New England Biolabs, Inc. BstXI – 5000 units
A restriction endonuclease that recognizes the sequence CCAN_NNNN^NTGG.
New England Biolabs, Inc. Topoisomerase I (E. coli) – 100 units
Topoisomerase I (E. coli) catalyzes the relaxation of negatively supercoiled DNA. Topoisomerase I has also been implicated in knotting and unknotting DNA and in linking complementary rings of single-stranded DNA into double-stranded rings. The intact holoenzyme is a 97 kDa protein.
New England Biolabs, Inc. BamHI – 10000 units
A restriction endonuclease that recognizes the sequence G^GATC_C.
New England Biolabs, Inc. PvuII – 25000 units
A restriction endonuclease that recognizes the sequence CAG_^CTG.