Enzymes and Inhibitors
Thermo Scientific™ FastDigest BspTI
Cut at C^TTAAG sites with Thermo Scientific™ FastDigest™ AflII (BspTI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ FastDigest HpaII
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ Hin1II (NlaIII) (5 U/μL)
Cut at CATG^ sites with Hin1II (NlaIII) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: CviAII, FaeI, FatI, Hsp92II, NlaIII).
Thermo Scientific™ FastDigest BclI
Cut at T^GATCA sites with Thermo Scientific™ FastDigest™ BclI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FbaI, Ksp22I).
R&D Systems™ Caspase-9 Inhibitor Z-LEHD-FMK
Used as cell-permeable, irreversible inhibitor of caspase activity. R&D Systems Caspase-9 Inhibitor Z-LEHD-FMK binds to the active site of activated proteases irreversibly and inhibits apoptosis.
Invitrogen™ DQ™ Collagen, type IV From Human Placenta, Fluorescein Conjugate
Analog of the natural substrate
Invitrogen™ EnzChek™ Cellulase Substrate, blue fluorescent, 339/452
Mixed with enzyme to produce fluorescent reaction
R&D Systems™ pERTKR-AMC Fluorogenic Peptide Substrate
Substrate for Furin, Proprotein Convertase 1 and 7, and wnvNS3
MilliporeSigma™ Calbiochem™ Y-27632
Y-27632A, CAS 146986-50-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency.
R&D Systems™ Mca-SEVNLDAEFRK(Dnp)RR-NH2 Fluorogenic Peptide Substrate
Applications: Bioactivity
MP Biomedicals™ Yeast Lytic Enzyme
From Arthrobacter luteus
MP Biomedicals™ Beta-Glucuronidase from Helix pomatia
Routinely used for the enzymatic hydrolysis of glucuronides from Serum plasma and other fluids prior to analysis by enzyme immunoassay, mass spectrometry, gas chromatography, high performance liquid chromatography, or other means.
Invitrogen™ ELF™ 97 Phosphatase Substrate (ELF™ 97 Phosphate), 0.2 μm Filtered
For use with microarray, flow cytometer and fluorescence microscope
Invitrogen™ PFB-FDGlu (5-(Pentafluorobenzoylamino)Fluorescein Di-β-D-Glucopyranoside)
PFB-FDGlu is a cell-permeable substrate used for live cell imaging of GCase activity. It fluoresces green upon cleavage by lysosomal GCase, facilitating specific, single-cell analysis via high-content imaging, flow cytometry, and microscopy.