Enzymes and Inhibitors
Invitrogen™ Anza™ DNA End Repair Kit
Used to convert DNA with cohesive ends to blunt-ended 5'-phosphorylated DNA for use in blunt-end ligation reactions
P-Nitrophenyl-α-D-Galactopyranoside, MP Biomedicals™
P-Nitrophenyl-α-D-Galactopyranoside, MP Biomedicals is used as a substrate for α-galactosidase.
MP Biomedicals™ Sorbitol Dehydrogenase, From Sheep Liver
An oxidoreductase in carbohydrate metabolism converting fructose into sorbitol
MilliporeSigma™ Upstate p300, HAT Domain Recombinant Enzyme
Recombinant GST-fusion protein corresponding to amino acids 1066-1707 of human p300.
Thermo Scientific™ AlfI
Cut at ^(10/12)GCA(N)6TGC(12/10)^ sites with AlfI restriction enzyme, which performs best at 37°C in R (+SAM) buffer.
Thermo Scientific Chemicals Caspase-3 Substrate V, fluorogenic
For cellular and molecular biology applications
MP Biomedicals™ Catalase from Beef Liver
Oxidoreductase enzyme for catalyzing conversion of hydrogen peroxide to water and oxygen, 2H2O2 → 2H2O + O2
R&D Systems™ Caspase-6 Inhibitor Z-VEID-FMK
Used as cell-permeable, irreversible inhibitor of caspase activity. R&D Systems Caspase-6 Inhibitor Z-VEID-FMK binds to the active site of activated proteases irreversibly and inhibits apoptosis.
Thermo Scientific™ FastDigest EheI
Cut at GGC^GCC sites with Thermo Scientific™ FastDigest™ EheI, which performs best using universal FastDigest Buffer (Isoschizomers: BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI).
Thermo Scientific™ FastDigest Van91I
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
MP Biomedicals™ Beta-Glucuronidase from Helix pomatia
Routinely used for the enzymatic hydrolysis of glucuronides from Serum plasma and other fluids prior to analysis by enzyme immunoassay, mass spectrometry, gas chromatography, high performance liquid chromatography, or other means.
Invitrogen™ TrueCut™ HiFi Cas9 Protein (5 μg/μL)
A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.
Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase), 1 U/μL
Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA